Polyglandular autoimmune syndromes (PAS) comprises a wide spectrum of autoimmune disorders for autoimmune inflammatory to invade.According to age of presentation,characteristic patterns of disease combinations,and different modes of inheritance,PAS are classified into four subtypes.Actual diagnosis of PAS involves clinical syndromes,serological measurement of organ-specific autoantibodies and subsequent functional testing.Principles of treatment included comprehensive assessment,hormone replacement,general consideration.

Objective To investigate metformin combined with insulin aspart 30 injection (NovoMix 30) in the treatment of type 1 diabetes mellitus (T1DM) in children and adolescents. Methods A total of 126 T1DM children over 10 years of age were randomly divided into insulin group (A) and insulin + metformin group (B). A group (n=60) was given insulin aspart 30 injection (insulin aspart 30), and B group (n=66) was given the metformin and insulin aspart 30 injection (NovoMix 30). Results The two groups can effectively control blood glucose, but the B group in the blood glucose control time, insulin dosage, the incidence of hypoglycemia, fasting blood glucose and hospitalization time were better than those of A group. There was no significant difference in liver and kidney function before and after oral administration of metformin in B group (P>0.05). Conclusion Metformin combined with insulin is effective and safe in the treatment of children with T1DM.

Objective To compare the therapeutic efficacy of two different administration routes of insulin administra?tion on juvenile type 1 diabetes mellitus (T1DM) complicated with diabetic ketoacidosis(DKA). Methods A total of 223 cases of juvenile T1DM was included in this study, among which 98 were complicated with DKA. Insulin was delivered through either continuous subcutaneous insulin infusion(CSII) by insulin pump or via multiple subcutaneous insulin injec?tion (MSII). Recovery period of blood glucose, insulin doses that were adminstrated, the urinary ketone bodies clearance time, the recovering time from DKA and the frequency of hypoglycemia incidence were all compared between these two routes. Results Both CSII and MSII routes reversed blood glucose and DKA effectively. However the recovering time of blood glucose and DKA, insulin dosage,the urinary ketone bodies clearance time and the frequency of hypoglycemia inci?dence all improved better or quicker in CSII than in MSII. Conclusion CSII by insulin pump is safer and more effective than MSII in the treatment of junvenile T1DM with metabolic disturbance and diabetic ketoacidosis.

Objective: To investigate the therapeutic effect of acupuncture plus hyperbaric oxygen in treating hypoxemic infantile encephalopathy (HIE). Methods: Fifty-nine HIE children were divided into treatment (33 cases) and control (26 cases) groups. Both groups were treated by basic therapy of removing acidosis, controlling cerebral edema and convulsion, and intravenous drip of cerebrolysin or citicoline. The treatment group was treated by acupuncture plus hyperbaric oxygen and the control group was treated by hyperbaric oxygen only. Results: The total effective rate was 97.0% in the treatment group and 73.1% in the control group. Statistical analysis showed a highly significant difference (P＜0.01). Conclusion: Acupuncture plus hyperbaric oxygen was better than simple hyperbaric oxygen in treating HIE. Its main manifestations were shortening the course of disease, increasing cure rate, decreasing death rate and reducing the occurrence of sequelae.HIE children should be treated as early as possible.

Objective To investigate the academic level and influence of the Chinese Journal of Digestive Surgery from the point of articles published and the authors.Methods The articles and authors of Chinese Journal of Digestive Surgery from 2002 to 2012 were retrieved from the Wanfang database and the China National Knowledge Internet database.The full texts were browsed and saved,and then the data were input to Excel for bibliometric analysis.Results There were 1917 articles were published in the Chinese Journal of Digestive Surgery during the past 11 years,and the average number of articles per issue was 29.05.The rate of articles with funded projects was 30.62％,and the citation rate was 58.52％.Authors were from 32 provinces,municipalites and Hong Kong.The numbers of articles contributed by authors from Chongqing,Beijing and Shanghai ranked top 3,which were 446(23.27％),240(12.52％) and 203(10.59％),and followed by authors from Sichuan,Jiangshu and Guandong.The number of articles from affiliated hospitals of Medical Schools was 1382 (72.09％).The cooperation rate was 4.37,and the coauthor rate was 90.03％.Conclusions Chinese Journal of Digestive Surgery is an academic journal with high quality and strong influence.The distribution of authors of the Chinese Journal of Digestive Surgery is wide,and the cooperative rate of authors is high,while the distribution of authors is disproportionate.

Purpose:To investigate the therapeutic effect a nd toxicity of radiotherapy plus ultrasound hyperthermia for treatment of malign ant tumors. Methods:41 cases of locally advanced, recurrent or radioresista nt cancer treated from January 1997 to January 2000 were given radiotherapy plus Sonotherm 1000 ultrasound hyperthermia. The mean radiation dose was 61?12 Gy;h yperthermia was administered within 30 minutes following radiotherapy with mean heating time of (52.4?6) minutes at 1-2 times/week. 3-year local control and survival were estimated by Kaplan-Meier method and Log rank test, factors affec ting prognosis were analyzed by Cox regression. The significance of differences between temperatures was tested by one-way ANOVA. Results:There was significant difference between Tmin and Tmax on tumor local response. Tumor complete response was 58.5%, the 3-year local co ntrol and survival were 13.6%% and 32.69% respectively. There is significant cor relation between radiation dose, tumor volume, Tmin and 3-year local control ( P

Whole blood samples were collected from 316 Uigur subjects inhabiting Kashi City, Sinkiang Uigur Autonomous Region, and their genomic DNA was extracted using QIAgen Blood Kit. DNA fragment spanning CCR5?32 and CCR5m303 mutation points were amplified by PCR and genotyped by directed electrophoresis and RFLP with HinCII respectively. In all 316 samples only 22 heterozygotes of CCR5?32 were found, in accordance with Hardy Weinberg equilirium. No CCR5m303 mutation was found. No difference existed between male and female individuals. The frequency of CCR5?32 in Uigur population is 3.48%, similar to Medi Asia ethnic groups (higher than that of Chinese Han people and lower than that of Caucasian people), i.e, in continuity regarding district distribution. Due to its slow down effect on clinical course of AIDS , high frequency of CCR5?32 is highly significant in prevention and treatment of AIDS in Uigur population.

Objective To detect the single nucleotide polymorphism(SNP) of chemokine RANTES promoter of healthy and HIV 1 infected individuals of Han and Uiygur Chinese population, and to evaluate the correlation of their SNPs with HIV 1 infection. Methods Case control study was adopted ,Genotypies of RANTES promoter 403 and 28 from 863 samples were detected by DNA sequencing or by polymerase chain reaction restriction fragment length polymorphism. Results There were two SNPs and six genotypes in Chinese Han and Uiygur. 403 and 28 allelic frequencies were high, but there was no significant difference between the two healthy populations. Strong linkage disequilibrium was observed between the two SNPs. RANTES genotypes were AC/AC, AC/AG, AC/GC, AG/GC, GC/GC, and AG/AG. The haplotypes were GC 62 7% in both rases, AC 28 7% and 30 4%, AG 8 6% and 6 8% in Han and Uiygur, respectively. Compared with AC/AC, odd ratio (OR) of RANTES genotypes AC/AG, AC/GC, AG/GC, and GC/GC was associated with reduced susceptibility to HIV 1 infection. Conclusion Same high RANTES promoter mutation was found in Han and Uiygur populations. Compared with AC/AC, odd ratio (OR) of RANTES genotypes AC/AG, AC/GC, AG/GC 9.9%, and GC/GC might be associated with reduced susceptibility to HIV 1 infection. Further study of the implicated in needed

Burnout, Professional ; Humans

Objective To investigate the expression of miR-101 in pancreatic cancer and the effect of down-regulation miR-101 on proliferation of pancreatic cancer cell line ASPC1. Methods Real-time PCR was used to determine the expression of miR-101 in pancreatic cancer, adjacent tissues and pancreatic cancer cell line ASPC-1. The miR-101 over-expression vector (peGFPc1-miR-101) was constructed and was transfected into ASPC-1 cell. Transfection efficiency was measured by fluorescence microscope. The expression of miR101in the transfected cells was detected by real-time PCR. Cell viability analysis was performed by MTT. The targeted genes of miR-101 in pancreatic cancer were scanned by the online targeted gene prediction software (target Scan). Results The expression of miR-101 was in pancreatic cancer tissues, adjacent tissues and ASPC-1 cell line, respectively. The expressions in pancreatic cancer tissues and ASPC-1 cells were significantly lower than that in adjacent tissues ( P ＜ 0.01 ). The expression of miR 101 in transfected cells increased to 19.8 folds as much as that in the control group (P ＜0.01 ). Proliferation rate of transfected cells was significantly decreased, which was only 26% of primary cells ( P ＜ 0.01 ). EZH2 was the potential targeted gene of miR-101 in pancreatic cancer. Conclusions miR-101 was weakly expressed and it may affect the proliferation of pancreatic cancer cell by inhibiting the EZH2 expression.