In early 2004, following an epidemic of avian influenza A/H5N1 in human, an outbreak of influenza A/H3 occurred in 5 communes at Hung Yen province with 887 cases and no deaths. This outbreak developed quickly but only in medium geographical range (inter-commune). Most cases are of school-age (from 5 to 19 years old) which comprise over 85% of the total number and focus mainly in primary and secondary schools. Owing to discovered early the outbreak, consisted of determinate the reasons and response to outbreak quickly and strongly and applied routinely to prevention the spread through the respiratory, and it also the good lesson to the prevention of outbreak
Disease Outbreaks ; Influenza, Human ; epidemiology

Introduction: There are thousands of diabetes sufferers worldwide. In addition, there is an increased trend in Vietnam due to economic development, increased population, lifespan, and changing lifestyle. Insulin is a hormone, which is a natural protein. It plays an important role in the transformation of glucose in human and animal blood. Note, while insulin has not been produced in Vietnam, the production of recombinant Proinsulin is a premise for insulin. This study is based on a successful design of pET - 28a (+) Vector with recombinant Proinsulin codified gene.\r\n', u'Objectives: To define the human proinsulin codified gene and study the optimum conditions for the expression of proinsulin. \r\n', u'Subjects and method: To discover the appropriate conditions for the expression of proinsulin, including initial cell density, cultural temperature, IPTG concentration, and expression time. The product of the expression was confirmed by Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SOS - PAGE) and reconfirmed by Western blotting with His - Tag antibody. \r\n', u'Results: Proinsulin expression was successfully proved by SOS - PAGE and Western blotting. Four appropriate conditions for the expression were confirmed as highlighted in the Conclusion. \r\n', u'Conclusion: The appropriate conditions for expression of proinsulin: cell density was 00600 0.6 - 1.0; the cultural temperature was 300C; IPTG concentration was 0.4 mM; the length of the culture time was 20 hours. \r\n', u'
Expression ; Proinsulin ; E.Coli ; Appropriate conditions