Animals ; Environment ; Female ; Male ; Maze Learning ; physiology ; Memory ; physiology ; Microarray Analysis ; Rats ; Rats, Sprague-Dawley ; Weaning

ObjectiveTo compare the difference of X ray projection volume and operative time when using two different positioning methods in percutaneous kyphoplasty to treat elderly osteoporotic vertebral compression fracture(OVCF) patients.MethodsSixty-eight(75 vertebraes) patients who underwent percutaneous kyphoplasty were divided into two groups,34 cases(39 vertebraes) underwent self-made fluoremeter (group A) comparing with the other 34 patients (36 vertebraes ) without using locator (group B).X ray projection volume,operation time and recovery of exterior edge height were compared.ResultsDuring locating the puncture point,the X ray projection volume in group A [( 196.92 ± 10.93)mA·s]was significantly less than that in group B [(826.67 ± 25.89 ) mA·s](P ＜ 0.05 ).The operation time in group A[(30.82 ±0.56) min]was significantly less than that in group B [(56.08 ±0.93) min](P＜ 0.05).The recovery of exterior edge height at 3 months after operation had no significant difference between two groups [(2.47 ± 0.18 ) mm vs.(2.09 ± 0.21 ) mm](P ＞ 0.05 ).ConclusionsThe self-made fluoremeter can save operation time and reduce the X ray projection volume.It is an effective way to treat OVCF patients undergoing percutaneous kyphoplasty.

To evaluate the clinical effect of laser in situ keratomileusis ( LASlK) with the corneal flap created by FEMTO LDV femtosecond laser for myopia.
●METHODS: The corneal flap was created by the FEMTO LDV femtosecond laser, and the thickness of the flap was 110μ m. A total of 143 myopic patients (283 eyes) were treated with the EC5000 - CXlll element laser. The optometry of the eye, best corrected visual acuity (BCVA) the thickness of the cornea, and ObscanⅡ were examined before the operation. The thickness of the flap was calculated by measuring the thickness of corneal bed during the operation in 35 eyes. The conditions of the corneal flap, complications, uncorrected visual acuity ( UCVA ), diopter, corneal topography were observed during and after the operation and were checked for 3mo follow-up.
●RESULTS: During the operation, it appeared small flap ( diameter < 5mm ) in 3 eyes, corneal margin incised incompletely in 5 eyes and incision bleeding in 8 eyes. Postoperative subconjunctival hemorrhage appeared in 6 eyes. The thickness of corneal flap in 35 eyes was 108. 75± 8. 52μ m (98-117μ m) and the error was 6. 49±8. 62μ m (3-12μ m). There was no significant difference between the actual flap thickness and the preset flap thickness ( P >0. 05) . The average equivalent spherical refractive was -0. 29± 0. 47 ( - 1. 50 to + 1. 00) DS after the operation for 3mo and the UCVA met or exceeded preoperative BCVA in 251 eyes (88. 7%).
●CONCLUSlON: The operation of myopia by LASlK flap created by FEMTO LDV femtosecond laser has fewer complications, and the effect is definite and safe.

The construction of art mass organization in medical colleges can consummate quality education system,make up insufficiency of the classroom teaching,promote medical students' comprehensive development and innovative ability and mold fine mind and lofty medical ethics.Therefore,it has important theoretical and practical significance to study existing problems and find out the ways to improve art mass organization construction of medical schools.

Traditional Chinese Medicine (TCM) is the gem of Chinese culture,while TCM culture the root and driving force for its development.Higher learning institutes,one of whose main missions is innovating and inheriting culture,are playing the unique roles in promoting world-wide recognition of TCM culture through international cooperation due to the abundant resources in TCM academy,talent,clinical study and international education.Taking Jiangxi University of Traditional Chinese Medicine as an example,this paper has made constructive exploration and study on how to promote the international communication of TCM culture to allow the international community for better understanding and the application of TCM based on the analysis of current situation and experience summarization with literature investigation and case study.Effective measures that have been put forward include establishing TCM experiencing center,promoting the construction of Confucius Institute,proactively building TCM library,improving the standardization of TCM translation,optimizing approaches of TCM international communication,and speeding up the cultivation of TCM international communication talents.

Adult ; Female ; Humans ; Occupational Diseases ; Plastics ; Trimethyltin Compounds ; poisoning ; Waste Management

Objective Comparing the difference in postoperative recurrence,complications,quality of life and cosmetic results between patients receiving radical mnastectomy and breast conserving surgery,provides an evidence of breast conserving surgery superior to radical mastectomy.Methods A retrospective analysis of 477 breast cancer patients cases in Department of General Surgery,Third People's Hospital of Baoji City from January 2009 to January 2012.These patients were divided into two groups:the control group 229 cases (48％) underwent conservative surgery treatment and the observation group 248 patients (52％) underwent radical surgery.Using SPSS15.0 statistical software analysis and compare with recurrence,postoperative complications,breast cosmetic effect and quality of life for these two groups of patients.Results In breast-conserving group compared with radical mastectomy group,the one and two year recurrence or metastasis rate were not statistically different between the two groups (P ＞ 0.05),the incidence of postoperative complications was significantly decreased (P ＜ 0.05).The scores for quality of life between the breast-conserving group and radical mastectomy group were all significantly different (P ＜ 0.05).Excellent cosmetic results in breast-conserving group was 78.52％,which was significantly higher than that in the radical mastectomy group (61.34％),the difference was statistically significant (x2 =5.86,P ＜ 0.05),The two groups are not significant in overall survival time (x2 =3.154,P ＞ 0.05) and progression free survival (x2 =4.243,P ＞ 0.05) as two indicator of long-term efficacy.Conclusions Conservative surgery compared with radical mastectomyhave less clinical complications,more breast cosmetic effect,better survival quality,and both of them share the same recurrence or metastasis and survival rate,so conservative surgery should be preferable in the clinical application.

The principles of step by step, the combination of practice and theory, the co-existing of being implicit and explicit, the equality between guidance and autonomous can be followed to construct humanities curriculum system in content, resources, teaching staff and teaching in medical colleges for nationalities. Furthermore, humanities curriculum system must insist on a fundamental concepts that the human theory is the essence of medicine, and the humanities is the basic structure of medicine and the key to success is the training of humanities teachers.

Abstract: Objective To analyze the plasma metabolomics of patients with occupational silicosis, and screen the differential Methods metabolic pathways in different stages of silicosis. Thirty patients with occupational silicosis were selected as silicosis group by judgment sampling method, including 10 patients in each subgroups with silicosis at stages Ⅰ, Ⅱ, and Ⅲ. Another 10 healthy individuals without occupational dust exposure history were selected as the control group. Plasma of each patientwascollected.Themetabolitesfromtheirplasmasampleswerecollectedandidentifiedbyuntargetedmetabolomicsusing----ultraperformanceliquidchromatographyquadrupoletimeofflightmassspectrometry.Principalcomponentanalysisandpartial least square discriminant analysis were used to compare the differentially expressed metabolites. The metabolic pathways Results enrichment analysis was performed using MetaboAnanlyst 5.0 and other metabolic analysis software. There were significantlydifferentmetabolicprofilesamongtheplasmaofthesilicosissubgroupsandthecontrolgroup.Inthepositivemode,--149differentmetaboliteswerescreened,amongwhich99metaboliteswereupregulatedand50metabolitesweredown regulated. Sphingolipids metabolism, arginine and ornithine metabolism, and fatty acid degradation are the main metabolic pathwaysinsilicosissubgroupswithstagesⅠandⅡcomparedwiththecontrolgroup.Themetabolicpathwaysofsilicosisstage Ⅲ are arginine and proline metabolism, glycerol metabolism, glyoxylic acid and dicarboxylic acid metabolism, and glycine, Conclusion serine and threonine metabolism. The plasma metabolic profile of patients with silicosis in different stages changed significantly. Sphingolipid metabolism is the main metabolic pathway in the early and middle stages of silicosis. In - silicosisterminalstage,thechangeofarginineandproline basedmetabolicpathwayresultinginfibrosisaggravation.

Objective Radionuclide-labeled low molecular weight polypeptide is reeently advocated for the diagnosis and treatment of malignant tumor. The purpose of this study was to evaluate the anti-tumor effect of 131Ⅰ-Tyr-octreotide in nude mice bearing human non-small cell lung cancer (NSCLC). Methods 131Ⅰ-Tyr-octreotide was prepared by Ch-T method. The radiochemical purity was measured and biodistribution was evaluated. The nude mice models bearing human NSCLC were studied and divided into four groups: group A injected 131Ⅰ-Tyr-octreotide through tail vein, group B injected normal saline, group C injected 131Ⅰ-Tyroctreotide through stroma and group D injected 131Ⅰ through stroma. The radioactivity ratio of tumor to normal tissue (T/NT) was calculated over region of interest (ROI). The tumor cell cycle and cell apoptosis were analyzed by flow cytometry (FCM), terminal deoxynucleotidyl transferase mediated dUTP-biotion nick end labeling (TUNEL) and histopathological analysis. Statistical analysis was performed with SPSS 11.0, and the comparison for difference between groups performed with one-way ANOVA analysis. Results The labeled radiochemical purity was (95.23±1.67)% and specific activity of 3.5×106Bq/ug. The biodistributiou showed high uptake in kidney, and low uptake in liver and spleen. The radioactive uptake in group C was higher than the other groups, and the retention time was longer. The T/NT was 52.74±0.13 after 24 h, which was much higher than that the other groups (group D: 8.90±0.23, group A: 6.42±0.02, q=628.81 and 664.33, all P<0.05). The resuits of tmnor cell cycle determined by FCM showed that the G1 phase was blocked mast remarkably in group C than the other groups [group C: (83.17±6.86)%, group A: (57.02±18.81)%, group D: (49.29±7.80)%, group B: (45.88±5.13)%, q=5.29, 6.86, 7.55, 1.56, 2.26, 0.69, all P<0.05]. Apeptotic cells were observed by TUNEL, and apoptotic body was detected by immuno-histochemical examination. Conclusions 131Ⅰ-Tyr-octreotide was easily labeled by Ch-T. 131Ⅰ-Tyr-octreotide could induce tumor cell apoptosis and inhibit the tumor cell of NSCLC. It might be a potential target-directed agent in NSCLC.