A model of intestinal helminth control program for primary school children was conducted during two years in Thai Nguyen province, where a high prevalence of intestinal worm had been found before the intervention. Results of the base line surveys showed a cumulative infection rate of intestinal worm of 86.23%, of which Ascaris: 83.6%, Trichuris: 20.6%, and hookworm: 3.9%. The cure rate was 97% (+/-2.52%) after intervention, but the reinfection rate was 53.11 % (+/-1.43%) by 4 months after the first treatment, and 35.88% (+/-1.85%) after the second treatment. Thus, periodical mass treatment every 6 months is needed for intestinal worm infection control. KAP surveys were performed to evaluate knowledge, attitude and practice of the children and their parents before and after intervention. The knowledge on the ways of transmission of worm infection and the role of environment among children and their parents was found to have been significantly improved immediately after the campaign of health education (P<0.05). Their attitude on the prevention of infection relating to the ways of infection, environment contamination and personal hygiene was enhanced significantly (P<0.05). Accordingly, their practice in term of intestinal worm infection control was also significantly enhanced (P<0.05), especially after the second round of health education and the school supervision. All families agreed to contribute budget to annual deworming program. The model was much supported by children, their parents and teachers for its high cost-effectiveness, easy application and safety, and therefore, it should be expanded to other places.
Helminths ; Child ; Therapeutics

Bdellovibrio bacteria BDF-H16 was isolated from the gut of Carassius auratus gibelio with Aeromonas sobria.Its shape was ob- served by light microscopy,phase-contrast microscopy,electron microscopy and some of its biological characteristics were also studied.It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2?m～0.5?m?0.8?m～1.2?m.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria.The best lysis conditions to Escherichia coli were 6.75?10~9 cfu/mL of prey bacteria concentration,pH7.0～7.5,28℃.It could grow in the solid culture added 0.85%～5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

Objective To analyze the problems of treating plague by TCM and find out the characteristics of clinical research of contemporary plague by the analysis of clinical research literature on the treatment of plague by TCM. Methods Differential treatment methods of one kind of plague in the literature as the main source of the data and the information that the pathogenesis-symptom-location of disease-nature of disease-prescriptions-drugs were taken to build the data base of the syndrome differentiation and treatment of certain kind of plague. Results Analyzed with literatures,the characteristics of plague clinical research showed: Different pathogenesis characteristics and evolution of plague were caused by diff① erent evils. ②Contemporary plague was named by western disease name. A new model which disease differentiation combined with syndrome differentiation has been formed. ③The diversification of administration promoted the treatment of emergent communicable disease with Chinese medicine. Conclusion The plague research lacked such research literature which could both reflect individualized diagnosis and embody the clinical rules. Traditional Chinese medicine theory could be researched and enriched by finding out the pathogenesis characteristics and evolution rule of the new plague and thus guide the clinical practice. It was effective to control and treat mergent communicable disease with TCM.

Objective:To explore the main economic and social factors influencing human health. Methods:By using the partial least squares(PLS) method and the panel data from areas of China in 2011, regression model related with economic social factors and human life expectancy was built. Results: Per capital GDP, the proportion of the tertiary industry, the number of people beyond college diploma per million people and the number of health technical personnel per 1 000 people has inverse relationshop with life expectancy;the proportion of medical costs accounted for fiscal expenditure, the ratio of fiscal deficit, rate of unemployment and Gini coefficient have reverse relationshop with life expectancy. Conclusion: To improve the level of human health, it needs to take comprehensive measures.

Aim To explore the effect of TRPV4 in thermoregulation.Methods We made the fever model with lipopolysaccharide in rat and used ruthenium red(RR) to inhibit the activity of TRPV4 to studied the content of TRPV4,cAMP and the concentration of [Ca2+]i.Result Given RR solely decreased the temperature Compared with LPS group,the increase amplitude of T,camp,[Ca2+]i all degrade in RR+LPS group And the content of TRPV4 are less than control group.Conclusion ① TRPV4 may participate the maintenance of normothermia;② The ferverscence was implemented by activating TRPV4 channel to induce a large calcium influx and then increasing the content of cAMP.

group N;?T(300～480 min) and TRI8 of group CPZ+LPS was significantly higher than that of group LPS(P

Objective: To explore the impact of regional economy on structure of total health expenditure. Methods: According to the panel data of China from 2002 to 2011, the partial least-squares regression method was used to build the relationship model of regional economy and total health expenditure construction. Results: Per capital GDP, the proportion of the tertiary industry, financial revenue per capita and urban residents disposable income have negative correlation with resident individual health expenditure, while the Engel coefficient has positive relationship with them. Conclusion: Optimizing industrial structure and raising the income of residents are the key factors of optimizing the structure of total health expenditure.

Objective To explore the surgical skills and evaluate the efficacy of single small incision herniorrhaphy for bilateral inguinal hernia. Methods From August 2006 to January 2013, 26 patients with bilateral inguinal hernia received single small incision herniorrha-phy,and the clinical data,including the operation time, hospital stay,postoperative complications and the recurrence,were analyzed retrospec-tively. Results In 26 cases of bilateral inguinal hernia,the operation time was (50. 6 ± 11. 6) min,the postoperative hospital stay was (5. 8 ± 1. 4) d,the incidence of postoperative complications was 3. 8%. No recurrence occurred during the follow-up of 10 to 58 months. Conclu-sion The single small incision herniorrhaphy for bilateral inguinal hernia is proved to be effective,safe and convenient,and it has few post-operative complications. This method is worth popularizing in the clinical practice.

Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is a breakthrough in the field of hepatobiliary surgery.The remnant liver regeneration was stimulated during the first stage of the operation,and the radical resection of the tumor could be successfully carried out during the second stage of the operation.ALPPS is a new approach for patients with tumor which is previously considered unresectable during one hospital stay.In April 2014,a patient with hepatocellular carcinoma (HCC) in the right liver and liver cirrhosis was admitted to the Southwest Hospital.Preoperative examination confirmed that the ratio of the remnant liver volume to the standard liver volume was 26.9％,which indicated that the patient was inappropriate to receive radical resection of HCC.Therefore,totally laparoscopic ALPPS was applied.In the first stage of the operation,the portal vein ligation associated with liver hanging tape placement and in situ splitting of liver were carried out after hanging the Glisson's pedicle under the laparoscope.Thirteen days after the first stage of the operation,the ratio of the remnant liver volume to the standard liver volume was 40.6％.The second stage of the operation was carried out 14 days after the first stage of the operation.The right hepatic pedicle and right hepatic vein were transected with a stapler.The tumor was removed after full mobilization of the right liver.The distance between the resection margin to the tumor was 1.5 cm.No complications were detected after the first stage of the operation,while the patient was complicated with pleural effusion after the second stage of the operation and was cured by pleural puncture and drainage.The patient was discharged at postoperative day 9 and followed up at postoperative month 1.The results of follow-up confirmed that the hepatic function was normal,and no mass occupying lesions and pleural effusion were detected.Totally laparoscopic ALPPS is safe and feasible with satisfactory short-term efficacy.

Objective To investigate the expression and purification I278T-mutant human cystathionineβsynthase(CBS) in E . coli .Methods Site-directed mutagenesis by overlap extension using the polymerase chain reaction (PCR) was employed to construct mutant plasmids pGEX4T-1-CBS(I278T) ,which was induced and expressed in a medium containing 3% ethanol ,purified by affinity chromatography to obtain mutated CBS (I278T) protein .The activity ,UV-visible absorption spectroscopy ,protein particle size and Zeta potential of the purified protein were measured .Results Plasmid pGEX4T-1-CBS(I278T) was successfully constructed .The yield ,the specific activity and activity recovery of purified mutant CBS (I278T ) protein were 2 .3 mg/L ,21 .4 U/mg and 22 .6% .S-adenosylmethionine(AdoMet) with final concentration of 1 mmol/L showed no activation toward mutant CBS (I278T) protein .Ac-cording to UV-visible absorption spectroscopy analysis ,purified mutant CBS(I278T) had characteristic absorption peaks at 429 nm and 550 nm for heme-binding proteins .Protein average particle size was 7 .5 -10 .1 nm ,mainly in the form of tetramers ,and Zeta potential was - 16 .3 mV .Conclusion The methods of expression ,purification and identification of I278T-mutant human cystathionineβsynthase in E .coli were successfully established .