Objective: To understand the iodine nutritional status of residents in the newly identified high iodine and non-high iodine areas in Puyang, and to provide evidence for relevant departments to adjust the iodine supplementation policy. Methods: According to the survey results of drinking water iodine content in 2017, in 2018 stratified and systematic sampling methods were used to extract 25 villages in the newly identified high iodine areas and non-high iodine areas in Puyang City. Each village surveyed 40 children and 20 pregnant women, the urinary iodine content of the subjects and the salt iodine content at their home were measured, and the thyroid volume of children in 10 villages in high iodine and non-high iodine areas was measured. Results: In high iodine areas and non-high iodine areas, the household salt samples collected were 1 357 and 1 544, respectively, the iodized salt coverage rate was 87.91% (1 193/1 357) and 85.62% (1 322/1 544), respectively, and the difference was not statistically significant (χ² = 3.291, P > 0.05). A total of 1 028 children and 329 pregnant women in high iodine areas were investigated, their median urinary iodine content was 337.0 and 289.0 μg/L, respectively; a total of 1 041 children and 503 pregnant women in non-high iodine areas were investigated, their median urinary iodine content were 310.0 and 265.0 μg/L, the differences of the urinary iodine content between high iodine and non-high iodine areas were statistically significant (Z = -3.825, -2.795, P < 0.05). Thyroid volume was measured in 407 children in high iodine areas and 423 children in non-high iodine areas. The thyroid enlargement rate was 8.85% (36/407) and 3.78% (16/423), respectively, and the difference was statistically significant (χ² = 9.053, P < 0.05). Conclusions The iodine nutrition levels of children and pregnant women in high iodine areas are higher than those in non-high iodine areas. Iodized salt should be stopped supplying in high iodine areas as soon as possible. In non-high iodine areas, the feasibility of reducing the iodine concentration of iodized salt should be investigated to ensure the iodine nutrition level of local residents.

The present study was aimed to investigate the influence of freeze-drying on the quality of polymerized human placenta hemoglobin (PolyPHb). The PolyPHb solution was freeze-drying under suitable conditions. Hemoglobin concentration, methemoglobin (MetHb) content, UV spectrum, Fe3 content, oxygen-carrying capacity, pH, the average molecular weight and its distribution, circular dichroism, oxygen equilibrium curve and other indicators were measured before and after freeze-drying. The appearance, residual water content, rehydration time of the lyophilized product were also evaluated. The results showed that there was no significant difference on all the indicators measured above, which indicated that freeze-drying process had no effect on the physical and chemical properties of PolyPHb, as well as on its biological activity. Therefore, the properties of PolyPHb were stable during this freeze-drying process and could be preserved after such freeze-drying process.
Blood Substitutes ; Female ; Freeze Drying ; methods ; Hemoglobins ; chemistry ; Humans ; Methemoglobin ; analysis ; Placenta ; chemistry ; Polymerization ; Pregnancy