1.Quantitative detection of Rabies virus antibody by chemiluminescent immunoassay
Chinese Journal of Clinical Laboratory Science 2006;0(02):-
Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application.
2.Synthesis and in vitro antibacterial activity of 7-(4-alkoxyimino-3-methyl-3-methylaminopiperidin-1-yl)quinolones.
Zhilong WAN ; Yun CHAI ; Mingliang LIU ; Huiyuan GUO ; Lanying SUN
Acta Pharmaceutica Sinica 2010;45(7):860-8
To explore new agents of quinolone derivatives with high antibacterial activity, 7-(4-alkoxyimino-3-methyl-3-methylaminopiperidin-1-yl)quinolones were designed and synthesized, and their activity against gram-positive and gram-negative strains was tested in vitro. Sixteen target compounds were obtained. Their structures were established by 1H NMR, HRMS and X-ray crystallographic analysis. Compounds 14k and 14m-14o show good antibacterial activity against the tested five gram-positive strains and five gram-negative strains (MIC: 0.25-16 micromg x mL(-1)), of which the most active compound 14o is 8-fold more potent than levofloxacin against S. pneumoniae (MIC: 4 microg x mL(-1)), and comparable to levofloxacin against S. aureus, S. epidermidis, E. faecalis and E. coli (MIC: 0.25-1 microg x mL(-1)), but generally less potent than gemifloxacin.
3.Influence of emergency PCI in different time windows to cardiac scar and cardiac function after myocardial infarction
Jincheng LIU ; Liying ZHANG ; Lanying KOU ; Hua GUO ; Wenzhong HAN ; Qinghai WANG
Clinical Medicine of China 2010;26(12):1277-1279
Objective To observe the influence of emergency PCI in different time windows to cardiac scar and cardiac function after myocardial infarction. Methods One hundred and twenty-seven patients with acute myocardial infarction, who were treated in different time windows by emergency PCI, were enrolled into this study. The cases were divided into two groups according to the time when they took PCI after onset;group 3 hPCI included 66 patients had PCI at 3 h after onset and group 6 hPCI included 61 patients had PCI at 6 h. At 4th, 8th and 12th weeks, the fragmented QRS (fQRS) were observed by twelve synchronization cardiogram; ventricle wall movement strain rate were determined by cardiac speckle tracking imaging;left ventricular ejection fraction (LVEF)were measured by cardiac ultrasound to evaluate cardiac events and cardiac function after myocardial infarction. Results At 4th,8th and 12th weeks,the fQRS positive leads were significantly more in 6 hPCI group than 3 hPCI group ( P < 0. 05 respectively ) . The ventricle wall movements strain rate and LVEF improved significantly in 3 hPCI group compared with 6 hPCI group (P < 0. 05 ). Conclusions The earlier emergency PCI treatment was applied and the earlier the"criminal"blood vessels were opened ,the more ischemic myocardial cells were restored. Combination use of fQRS、 STI and LVEF examination could improve the diagnosis of cardiac scar formation and direct protective therapy.
4.In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV
Ruofeng SHANG ; Jianping LIANG ; Zhongyuan NA ; Hongjun YANG ; Yu LU ; Lanying HUA ; Wenzhu GUO ; Ying CUI ; Ling WANG
Virologica Sinica 2010;25(2):145-150
NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.
5.Artificial Calculus Bovis inhibits neuron loss in hippocampus and hilus and protects the GAD positive cells in hippocampus of epileptic rats
Jiefang LIANG ; Jingxin HU ; Binyuan YANG ; Shengqiang CHEN ; Yu ZHONG ; Yujuan LIANG ; Guangfei DENG ; Beihua ZHONG ; Zhe HUANG ; Lanying HUANG ; Lanlan GUO
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To probe into the anti-epilepsy action of artificial Calculus Bovis,by observing its effect on the behavioral of the experimental epileptic rats,neuron loss in the hippocampus and hilus,and GAD positive cell alteration in the hippocampus.METHODS: SD rats were divided into three groups: group A(artificial Calculus Bovis treatment group);group B(acute epilepsy group) and group C(control group).A model of acute epilepsy rats was established by PTZ.The rat's behavioral alteration was observed by the Racine' scale.The neurons in the hippocampus and hilus were calculated by Nissl staining.The GAD positive cells were observed by immunohistochemical staining.RESULTS: The latency of the first seizure in group A was longer than that in group B,while the seizure times in group A was less than that in group B.Besides,in group A,both the neuron loss amount in the hippocampus and hilus and the GAD positive cell loss amount in the hippocampus were less than those in group B.CONCLUSION: The artificial Calculus Bovis prolonged the latency of the first seizure time,decreased the frequency of seizure,and prevented the neuron loss and protected the GAD positive cells.
6.Effect of exercise therapy on exercise capacity and quality of life of maintenance hemodialysis patients
Xiumei LI ; Hao LI ; Lanying GUO ; Tengwei ZHANG ; Hongmei ZHANG
Chinese Journal of Practical Nursing 2022;38(28):2178-2183
Objective:To explore the effect of exercise therapy on the exercise capacity and quality of life of maintenance hemodialysis patients.Methods:A total of 90 patients who underwent regular dialysis in Henan Provincial People′s Hospital, People′s Hospital of Zhengzhou University from April to December, 2020 were selected as the research objects by convienient sampling method. They were divided into experimental group and control group by random number table method, with 45 cases in each group. The control group was given routine nursing, and the experimental group took exercise therapy on the basis of routine nursing. The intervention lasted for 12 weeks. The changes in the exercise capacity and quality of life of the two groups before and after the intervention were observed.Results:Finally, 43 patients were included in the control group and 41 patients in the experimental group. There was no significant difference in the exercise capacity and quality of life before the intervention between the two groups( P>0.05). After the intervention, the maximum oxygen uptake, anaerobic threshold, 6MWT distance were (15.22 ± 3.08) ml·kg -1·min -1, (620.70 ± 53.56) ml·kg -1·min -1, (475.36 ± 50.51) m in the experimental group, and (12.22 ± 2.46) ml·kg -1·min -1, (590.18 ± 52.07) ml·kg -1·min -1, (444.50 ± 46.09) m in the control group, the differences were statistically significant between the two groups( t=3.32, 2.68, 296, all P<0.05). After the intervention, the scores of physiological function, physiological intelligence, physical pain, overall health, vitality, social function, emotional function and mental health dimensions of the quality of life were (84.32 ± 18.53), (84.61 ± 25.80), (76.40 ± 18.15), (59.02 ± 15.38), (52.22 ± 13.68), (69.56 ± 17.29), (94.24 ± 29.47), (73.44 ± 12.86) points in the experimental group, and (76.25 ± 17.35) (66.37 ± 27.63), (65.68 ± 17.54), (47.26 ± 15.45), (44.86 ± 13.14), (61.82 ± 16.51), (76.30 ± 26.14), (64.17 ± 13.36) points in the control group, the differences were statistically significant between the two groups( t values were 2.08-3.54, all P<0.05). Conclusions:Exercise therapy during dialysis can enhance exercise capacity and improve quality of life in maintenance hemodialysis patients.
7.Thoughts on the Formulation of"2023 Guidelines for Diagnosis and Treatment of Depression with Integrative Chinese and Western Medicine"
Liyuan GUO ; Jianjun WANG ; Zhen WANG ; Lanying LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(1):12-18
The integration of Chinese and Western Medicine(ICWM)in the diagnosis and treatment of depression has been a long-standing issue in the field of mental health.In order to maximize the benefits of this combined approach,the"2023 Guidelines for Diagnosis and Treatment of Depression with Integrative Chinese and Western Medicine"(GDTD-ICWM)has been developed based on organizing evidence-based and standardized workflows for ICWM.This article aims to discuss the background,highlights,summary and outlook of the"Guidelines",as well as offer operational suggestions to guide clinical practices in order to strengthen the implementation,practice and promotion of the"Guidelines".
8.Efficacy and Safety of Acupuncture in Treating Major Depressive Disorders:A Systematic Review and Meta Analysis
Wangtao LI ; Mingqi TU ; Liyuan GUO ; Yiyin HU ; Haomin SUN ; Peiqi LI ; Lanying LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(1):76-87
Objective To evaluate the effectiveness and safety of acupuncture in patients with Major Depressive Disorders.Methods Search the database for relevant literature published before April 15,2023.Select randomized controlled trial that meeting the enrollment criteria,evaluate the quality of the literature and classify before meta-analysis systematic evaluation.Results 15 RCTs are included in this study,with a total of 1077 patients.The results showed that acupuncture treatment,compared with antidepressants alone,The score of Hamilton Depression Scale(HAMD)in patients with liver-qi depression disorder[MD=-0.73,95%CI(-1.22,-0.24),P<0.05],the score of TCM Syndrome Scale[MD=-1.89,95%CI(-2.35,-1.44),P<0.001],effective rate[RR=1.09,95%CI(1.03,1.153),P=0.001]and TESS score[MD=-2.76,95%CI(-3.68,-1.85),P<0.001]were statistically significant.Compared with fluoxetine hydrochloride,the effective rate of HAMD scale score was statistically significant[RR=1.07,95%CI(1.01,1.14),P<0.05].Conclusion Compared with antidepressants alone,acupuncture therapy has the advantages for the improvement of HAMD score in patients,the improvement of Traditional Chinese medicine symptoms,and significantly less adverse effects than those in the control group.Through this study,it is considered that acupuncture can be a safe and effective treatment modality.However,due to the quantity and quality of the included literature,the above conclusions still need to be verified by more high-quality clinical trials.
9. Clinical and variant analysis of 15 patients with methylmalonic acidemia
Hui XIONG ; Wenlin DENG ; Lanying GUO ; Congcong SHI ; Xin XIAO ; Hu HAO
Chinese Journal of Medical Genetics 2019;36(12):1158-1162
Objective:
To report on clinical characteristics and genetic findings in 15 Chinese patients with methylmalonic acidemia (MMA).
Methods:
For the 15 MMA patients detected by tandem mass spectrometry, genetic analysis was carried out in twelve pedigrees. Clinical characteristics, genetic finding, treatment and outcomes were retrospectively analyzed.
Results:
The main features of the patients included poor feeding, recurrent vomiting, lethargy, seizure and development retardation. Blood propionylcarnitine (except for 3 patients), its ratio with acetylcarnitine, and urine methylmalonic acid were increased in all patients. Twelve patients were diagnosed genetically, which included 7 with
10.Construction of the Chinese Veteran Clinical Research (CVCR) platform for the assessment of non-communicable diseases.
Jiping TAN ; Nan LI ; Jing GAO ; Yuhe GUO ; Wei HU ; Jinsheng YANG ; Baocheng YU ; Jianmin YU ; Wei DU ; Wenjun ZHANG ; Lianqi CUI ; Qingsong WANG ; Xiangnan XIA ; Jianjun LI ; Peiyi ZHOU ; Baohe ZHANG ; Zhiying LIU ; Shaogang ZHANG ; Lanying SUN ; Nan LIU ; Ruixiang DENG ; Wenguang DAI ; Fang YI ; Wenjun CHEN ; Yongqing ZHANG ; Shenwu XUE ; Bo CUI ; Yiming ZHAO ; Luning WANG
Chinese Medical Journal 2014;127(3):448-456
BACKGROUNDBased on the excellent medical care and management system for Chinese veterans, as well as the detailed medical documentation available, we aim to construct a Chinese Veteran Clinical Research (CVCR) platform on non-communicable diseases (NCDs) and carry out studies of the primary disabling NCDs.
METHODSThe Geriatric Neurology Department of Chinese People's Liberation Army General Hospital and veterans' hospitals serve as the leading and participating units in the platform construction. The fundamental constituents of the platform are veteran communities. Stratified typical cluster sampling is adopted to recruit veteran communities. A cross-sectional study of mental, neurological, and substance use (MNS) disorders are performed in two stages using screening scale such as the Mini-Mental State Examination and Montreal cognitive assessment, followed by systematic neuropsychological assessments to make clinical diagnoses, evaluated disease awareness and care situation.
RESULTSA total of 9 676 among 277 veteran communities from 18 cities are recruited into this platform, yielding a response rate of 83.86%. 8 812 subjects complete the MNS subproject screening and total response rate is 91.70%. The average participant age is (82.01±4.61) years, 69.47% of veterans are 80 years or older. Most participants are male (94.01%), 83.36% of subjects have at least a junior high school degree. The overall health status of veterans is good and stable. The most common NCD are cardiovascular disorders (86.44%), urinary and genital diseases (73.14%), eye and ear problems (66.25%), endocrine (56.56%) and neuro-psychiatric disturbances (50.78%).
CONCLUSIONWe first construct a veterans' comprehensive clinical research platform for the study of NCDs that is primarily composed of highly educated Chinese males of advanced age and utilize this platform to complete a cross-sectional national investigation of MNS disorders among veterans. The good and stable health condition of the veterans could facilitate the long-term follow-up studies of NCDs and provide prospective data to the prevention and management of NCDs.
Aged ; Aged, 80 and over ; Asian Continental Ancestry Group ; Cross-Sectional Studies ; Disease ; Female ; Health Status ; Humans ; Male ; Veterans ; statistics & numerical data