Objective　The aim of this paper was to investigate the reliability and validity of the Chronic respiratory disease questionnaire (CRQ) in China. Method　Sixty-eight patients with chronic respiratory disease who were in hospital from January to November, 1999 were surveyed with CRQ. Eighteen patients were investigated repeatly with CRQ in one week to test the reliability; before and the 10th day after treatment, 50 patients were surveyed twice with CRQ and the peakflow of these patients were also detected. The same doctor explained questionnaires and tested peakflow. Results　The correlated analysis of reliability was positive (r=0.732, P<0.01). The correlatied analysis between the difference of twice-questionnaire scores and the difference of twice-peakflow value was also positive (r=0.565, P<0.01).Conclusion　CRQ can be used by the clinical doctors of China.

Objective To investigate the protective effects of soybean isoflavone(SI) on genetic toxicity induced by di-n-butul phthalate(DBP) in mice.Method(1) Micronucleus test:40 male 7 w old Kunming mice were randomized into 4 groups:High and low dose SI intervention groups,DBP model group,and solvent control group.SI intervention groups were given different doses of SI(50,100mg/kg) for 30 d,meanwhile,the DBP group and solvent group were given 0.5% sodium carboxymethyl cellulose.Then all groups were treated by 0.5g/kg DBP for 5d except solvent group.Mice were sacrificed 6 hour after last treatment,and then counting micronucleated cells in bone marrow.(2) Sperm malformation test:40 male 6w old Kunming mice were grouped and treated the same as micronucleus test.Mice were sacrificed at 35 day after the first treatment,and then sperm quantity,motility,viability and abnormality rate were calculated.Result Micronucleus rate and sperm abnormality rate of SI intervention group were lower than DBP model group,while sperm motility and viability were higher than DBP model group.Conclusion SI can relieve the genetic toxicity induced by DBP in mice.

OBJECTIVEThe purpose of this study was to investigate the relationship between the results of flowcytometry analyses of different clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkin's lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.

METHODThis study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).

RESULTSReactive lymph nodes were all diploid. The diploid rate of NHL was 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHL in the DNA ploidy status and cell cycle data (SPF, CV, S + G2/M, DI). The S phase fraction (SPF) and S + G2/M had close relationship with the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.

CONCLUSIONThe results suggested that the FCM had diagnostic value in NHL, especially when the morphological diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF + G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.

Cell Cycle ; DNA, Neoplasm ; analysis ; Facial Neoplasms ; diagnosis ; genetics ; pathology ; Flow Cytometry ; Humans ; Lymphoma, Non-Hodgkin ; diagnosis ; genetics ; pathology ; Maxillary Neoplasms ; diagnosis ; genetics ; pathology ; Mouth Neoplasms ; diagnosis ; genetics ; pathology ; Ploidies ; Prognosis

Objective: To develop a new identification and analysis method of crude drug Pheretima . Method: Powders of pheretima were identified by means of X ray differacion Fourier pattern. Results: Experiments and analysis were carried out on five samples of pheretima. The standard X ray diffraction Fourier pattern and characteristic diffraction peaks of Pheretima were obtained.Conclusion: This method can be used for identification on crude drug Pheretima .

OBJECTIVEThe purpose of this study was to screen sialyl Lewis(a) (sLe(a)) in the tumors of patients with oral squamous cell carcinoma (OSCC) and to explore the association of sialyl Lewis(a) expression with local lymph node metastasis.

METHODSSpecimen from 38 patients with primary OSCC were obtained and analyzed by immunohistochemical methods.

RESULTSThe expression of sLe(a) protein, but not E-selectin, of OSCCs significantly correlated to the local lymph node metastasis. sLe(a) was expressed in 79% (15/19) of the metastatic cases compared with 21% (4/19) of the non-metastasis ones, indicated the association of sLe(a) expression with the local lymph involvement.

CONCLUSIONHigh expression of sLe(a) in OSCC may be related to the metastasis of cervical lymph nodes and it seems useful in predicting poor prognosis in OSCC.

Adult ; Aged ; Antigens, Tumor-Associated, Carbohydrate ; analysis ; Biomarkers, Tumor ; analysis ; Carcinoma, Squamous Cell ; chemistry ; genetics ; pathology ; secondary ; E-Selectin ; analysis ; genetics ; Female ; Gangliosides ; analysis ; genetics ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Middle Aged ; Mouth Neoplasms ; chemistry ; genetics ; pathology

OBJECTIVEThe aim of this study was to investigate the association between clinical and pathological characteristics of primary oral mucosal malignant melanoma and the prognosis of this disease.

METHODSClinical and pathological characters of 73 cases primary oral mucosal malignant melanoma were investigated. The association between risk factors, such as black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and prognosis was analyzed using Kaplan-Meier survival analysis and Log rank test.

RESULTSThe age of the patients ranged from 24 to 80 years (Median age, 50). Among the patients, 43 were males and 30 were females. The most common locations of the tumor were palate and gingiva. The clinic stages of these patients were as the following: Stage I (46/73), Stage II (24/73), Stage III (3/73). The most common pathological type was nodular (44/73), followed by lentiginose malignant melanoma (15/73) and superficial spreading (1/73). According to the configuration of tumor cells, the most common type was the mixed cell type (37/73), followed by the epitheloid cell type (27/73) and the spindle cell type (9/73). Among the 73 patients, 43 were followed up, the 3-year and 5-year survival rates were 19.86% and 11.91% respectively. Black macule, lymph node metastases, invasive depth, clinic stages, pathologic types and therapeutic methods were significantly associated with the prognosis (P < 0.05).

CONCLUSIONThe prognosis of the primary oral mucosal malignant melanoma is associated with black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and therapeutic methods.

Adult ; Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Humans ; Lymphatic Metastasis ; Male ; Melanoma ; pathology ; Middle Aged ; Mouth Mucosa ; pathology ; Mouth Neoplasms ; pathology ; Neoplasm Invasiveness ; Neoplasm Staging ; Prognosis ; Retrospective Studies ; Survival Rate

OBJECTIVETo study clinicopathological features, diagnosis, differential diagnosis of oral Langerhans cell histiocytosis (LCH), retrospective clinicopathologic study was carried on and a variety of immune phenotype were detected.

METHODSThe clinicopathological features of 29 cases of oral LCH were analyzed. The immunohistochemical staining of S-100 protein, CD1a, CD83 and Ki-67 were used in above cases by immunohistochemical streptavidin-biotin peroxidase (SP) and Elivison two-step method. Statistical analysis was adopted for the results.

RESULTSOf the 29 cases of LCH, the expression of S-100 protein and CD1a were positive in 24 cases and negative in 5 cases, so 5 cases were excluded from the diagnosis of LCH. Among 24 cases of LCH, 15 patients were male and 9 were female. The median age was 7.50 years. 14 lesions were in the mandible, 5 were in the maxilla and 5 involved the mandible and maxilla. 9 cases were in stage I, 13 in stage II and 2 in stage III, according to Bartnick classification. Immunohistochemistry showed all 24 cases staining for S-100 protein and CD1a were positive. Comparing with maxillofacial lesions involved soft tissue, Ki-67 positive rate was lower and CD83 positive rate was higher in maxillofacial single bone lesion.

CONCLUSIONThe immunohistochemical staining of S-100 protein and CD1a are important for the diagnosis of LCH. Maxillofacial bone single LCH might have lower proliferative activity and a higher state of maturity. Maxillofacial LCH involved soft tissue might have a higher proliferative activity and a lower state of maturity.

Antigens, CD1 ; Diagnosis, Differential ; Female ; Histiocytosis, Langerhans-Cell ; Humans ; Immunohistochemistry ; Male ; Mandible ; Maxilla ; Retrospective Studies ; S100 Proteins

Mucinous adenocarcinoma is a rare epithelial malignant tumor which usually arises in appendix, pancreas, breast and other sites, rarely occurs in salivary gland. In this article, a mucinous adenocarcinoma of salivary gland was reported and relevant literatures were reviewed.
Adenocarcinoma, Mucinous ; Carcinoma ; Humans ; Salivary Glands

White sponge nevus (WSN) is a rare, autosomal-dominant hereditary disease. This article reported two siblings affected by white sponge nevus. Because of smoking or not, they presented different clinical manifestations.
Humans ; Leukokeratosis, Hereditary Mucosal ; Nevus ; Siblings

Objective: To explore the influence of adverse childhood experiences (ACEs) on pubertal development of boys and girls and to provide a reference for the development of intervention measures. Methods: A stratified cluster sampling method was used to select a total of 1 156 students in grades three and four in the boarding school system and public primary schools in Huangshan City and surrounding towns in September 2018, using the Childhood Trauma Questionnaire (CTQ) and the Pubertal Development Scale (PDS). For the baseline self-assessment survey, according to different dimensions, abuse children score no exposure groups. Children were divided into an exposure group and a high exposure level group, according to their childhood experiences. PDS self-report questionnaire was administered two years later, and an analysis of ACE type and severity of the continuous impact of youth development was conducted. Results: In the baseline survey, there were 53 girls (11.32%) and 51 boys (7.41%) who developed earlier. The rate of early development in girls was higher than that of boys, and the difference was statistically significant(χ 2=5.21, P<0.05). Univariate analysis showed gender differences in the effects of type and severity of ACEs and abuse on adolescent development at both baseline and follow-up. There were gender differences in the rate of early development between boys and girls at baseline and at follow-up between the exposure groups. Regression analysis showed that the higher the degree of emotional abuse, emotional neglect, and sexual abuse in girls, the higher the PDS score(B=0.22, 0.15, 0.08, P<0.05). In boys, the more severe the emotional abuse experienced, the higher the PDS score, and the more severe the physical abuse experienced, the lower the PDS score(B=0.20, 0.04, P<0.05). Conclusion Attention should be paid to the influence of ACEs and gender differences during youth development among male and female students, and more longterm studies should also be carried out.