1. MicroRNA-221/222 participates in the pathogenesis of intrahepatic cholestasis of pregnancy via promoting the apoptosis of human placental trophoblast HTR-8 cells
Chinese Journal of Hepatology 2018;26(8):607-611
Objective:
MicroRNA-221/222 is involved in the pathogenesis of intrahepatic cholestasis of pregnancy (ICP) to promote the apoptosis of placental bile acids through human trophoblastic cells. This study investigates the effects of miR-221/222 on proliferation, apoptosis and apoptosis-related proteins of human trophoblast HTR-8/SVneo (HTR-8 cells) to understand its role in promoting trophoblastic apoptosis.
Methods:
The experiment was divided into transfection group and negative control group. Transient transfection method was used in both groups. The transfection efficiency was detected by RT-QPCR after 48 h transfection. CCK-8 was used to detect the proliferation of HTR-8 cells and the apoptosis of HTR-8 cells were analyzed by flow cytometry. Western blot was used to detect the expression of B-cell Lymphoma 2 (Bcl-2) in HTR-8 cells. Data were compared with t-test.
Results:
The expression of miR-221/222 transfected group (25.43±0.80, 22.70±0.95) was increased significantly in the HTR-8 cells than that to negative control group (1.14±0.14, 1.58±0.14), and
2.Simultaneous HPLC determination of 11 essential compounds in Xuebijing injection.
Lanxin JI ; Hao HUANG ; Min JIANG ; Gang BAI ; Guoan LUO
China Journal of Chinese Materia Medica 2010;35(18):2395-2398
OBJECTIVETo establish a HPLC method for the determination of 11 essential compounds in Xuebijing injection.
METHODChromatogaphic analysis was performed on Aglient Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm). The mobile phase 0. 5% acetic acid and methanol-acetonitrile-acetic acid (40: 60: 0.5). The flow rate was 1.0 mL x min(-1) with the detection wavelength at 280 nm; column temperature at 35 degrees C, and automatic sampling volume of 20 microL.
RESULTAll the 11 essential compounds showed good linearity (r = 0.9982-0.9999)in the range of the tests concentration. The RSD of the precision, reproducibility and stability tests were less than 3%; the average recoveries of the method were in the range of 95.02%-104.94%.
CONCLUSIONThe method is simple, rapid and accurate, so it is proposed for the quality control of compounds of Xuebijing injection.
Chromatography, High Pressure Liquid ; methods ; Dosage Forms ; Drugs, Chinese Herbal ; analysis