Acupuncture Points ; Adult ; Aged ; Female ; Ginger ; chemistry ; Humans ; Male ; Middle Aged ; Moxibustion ; Stroke ; complications ; Urinary Incontinence ; etiology ; physiopathology ; therapy ; Urination

This paper summarized the epidemiology and management of Chinese andWestern Medicine for voiding disorders.

Cognitive impairment after stroke is one of the common problems, that affects the physical, behavioural and emotional rehabilitation, etc. There is no effective means of Western medicine. This article reviewed understanding, medication, acupuncture and moxibustion for cognitive impairment in view of Chinese Medicine.

Acupuncture for stroke rehabilitation is of multi-targets, the treatments of which are not only for hemiplegia, but also cover a wide variety of aspects, such as aphasia, dysphagia, dysarthria, cognitive disorders, bowel or bladder dysfunction, ataxia and post hemiple-gic pain, etc. Based on the experiences of stroke rehabilitation with Integrated Traditional Chinese and Western Medicine over 20 years, this paper discussed the selection of acupoints, needling manipulations, opportunity of intervention, quantity of stimulations, the needle-retaining time as well as the impact on spasticity after hemiplegia referring to ancient and modern literatures and modern rehabilitation theory.

Objective:To observe the therapeutic effect and mechanism of Huzhangyuzhuo decoction on treating chronic pelvic pain syndrome(CPPS).Methods:46 male Wistar rats were randomly divided into control group(n=10) and experiment group(n=36).The CPPS rat model was induced by intraperitoneal injection of diphtheria-pertussis-tetanus vaccine and subcutaneous injection of puri ed prostate protein with FCA suspension.Rats in experiment group were subdivided into model group,Chinese medicine group and western medicine group(n=12).After 30 days of treatment,pathology changes of prostate were observed,and levels of IL-1?,COX-2,PGE2,?-EP in prostate homogenate were measured by ELISA.Results:Huzhangyuzhuo decoction greatly reduced the in ammation of prostate and relieved the structure damage.At the same time,Huzhangyuzhuo decoction can decrease the level of IL-1?,COX-2,PGE2 and increase level of ?-EP.Conclusions:Huzhangyuzhuo decoction might treat CPPS by regulating of cytokines in the prostate,depressing in ammation and relieving structure damage.

OBJECTIVE To investigate dopamine level changes in the Nucleus Accumbens (NAc) of the limbic system by salicylate and the role of dopamine in the mechanisms of tinnitus.METHODS We recorded the change of dopamine before and after sodium salicylate injection in rats by microdialysis combined with electrochemical methodologies in the nucleus accumbens (NAc).RESULTS Salicylate produced significant increase in dopamine level in the NAc, to a maximum of (321±97)% of baseline and then remain stable. In contrast, saline only produced slight increase.CONCLUSION The increase of dopamine in the NAc may be related to the generation of tinnitus. The data in vivo provide direct evidence of the important role that the dopaminergic system plays in the generation of tinnitus.

Objective To investigate the effects of electroacupuncture on head Shen- acupoints on cognitive dysfunction after stroke. Methods 35 cases with cognitive dysfunction after stroke were divided into treatment group (n=19) and control group (n=16). The control group received conventional treatment, while the treatment group received electroacupuncture on Sishencong (EX-HN1), Shenting (GV24) and Benshen (GB13) in addition. They were assessed with Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) before and after 4-week treatment. Results The scores of MMSE and MoCA increased more in the treatment group than in the control group (P<0.05). The scores of visuospatial abilities and executive functions, attention and concentration, calculation, memory and orientation of MoCA improved more in the treatment group than in the control group (P<0.05). Conclusion Electroacupuncture on head Shen-acupoints can improve cognitive function in patients after stroke.

Objective To explore the relationship between temperature and change of serum electrolytes (potassium, natrium, chlorine,and calcium) in patients with spinal cord injury. Methods 321 patients with spinal cord injury (SCI) following fever were regarded as observation group, while 313 SCI patients in normal temperature as control group. The level of serum electrolytes (potassium, natrium, chlorine, and calcium) in both groups was analyzed. Results There was no significant difference in the level of serum potassium (P>0.05), but was in the serum natrium, chlorine, and calcium (P<0.05) between 2 groups. The level of serum potassium didn't correlated with their temperature, and the level of serum natrium, chlorine, and calcium correlated with the temperature negatively. Conclusion The level of serum natrium, chlorine, and calcium may decrease in SCI patients following fever while the level of serum natrium is unchanged.

A new magnetic affinity immunoassay (MAIA) strategy based on enzyme-labeled phage displayed antibody was developed. The assay consisted of a sandwich format in which immobilized polyclonal antibody (pcAb) on magnetic microparticle was used for capture probe, and enzyme-labeled phage displayed antibody for specific detection probe to increase enzyme amount and enhance detection signal. By the proposed method,β-bungarotoxin (β-BGT) was successfully detected. A linear relationship between absorbance value and the concentration of β-BGT in the range of 0. 016-62. 5 μg / L was obtained. The linear regression equation was Y=0. 641X+1. 355 (R =0. 9925, n = 13, p<0. 0001) with a detection limit of 0. 016 μg / L. In comparison with the traditional ELISA, this method gave a 10-fold better sensitivity in β-BGT detection. This strategy also gave a 4-fold better sensitivity comparing with the MAIA based on enzyme labeled monoclonal antibody (mcAb). Due to low detection limit, acceptable reproducibility and high specificity, this method holds great promise in toxin trace detection.

A new method for O-ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate ( VX) , sarin detection and its kinetic analysis based on piezoresistive microcantilever aptasensor was developed, where VX, sarin aptamers were immobilized on the microcantilever surface by biotin-avidin binding system. A linear relationship between the response voltage and the concentration of VX in the range of 2-60μg/L was obtained. The linear regression equation was △Ue=0. 886C-1. 039 (n=5, R=0. 984, p<0. 001) and the detection limit was 2μg/L ( S/N≥3 ) . A linear relationship between the response voltage and the concentration of sarin in the range of 10-60 μg/L was obtained, the linear regression equation was △Ue=0. 716C-2. 304 ( n=5, R=0 . 996 , p<0 . 001 ) and the detection limit was 10 μg/L ( S/N≥3 ) . The sensor showed no response for O-butyl methylphosphonochloridate, a structural analog of VX and sarin, which indicated high specificity and good anti-interference ability. On this basis, a reaction kinetic model based on receptor-ligand binding and the relationship with output voltage change was established. Response voltage (△Ue ) and response time( t0 ) were obtained from the fitting equation on different concentrations of VX, sarin fitted well with the measured values.