Objective To observe the changes of high sensitive C reactive protein(hs-CRP)and blood lipids,and investigate the clinical value of combined detection of the above markers in evaluating the risk and prognosis of coronary heart disease(CHD).Methods Totally 51 cases of stable angina pectoris(SAP group),44 cases of unstable angina pectoris(UAP group),40 cases of acute myocardial infarction(AMI group)and 45 healthy controls(healthy control group)were enrolled in the investigation.Hs-CRP and blood lipids were measured with enzymic method and immunoturbidimetry respeetively.Results The level of hs-CRP was significantly higher in UAP group and AMI group than that in healthy control group(both P＜0.05).Hs-CRP level was a little higher in SAP group than that in healthy control group,but there was no statistical difference(P＞0.05).In addition,hs-CRP was positively correlative with total cholesterol(TC),low density lipoprotein-cholesteroK LDL-C),apoprotein B (apoB)and lipoprotein a(LPa)(P＜0.05),and negatively correlative with high density lipoproteincholesterol(HDL-C)and apoAI(P＜0.05).Conclusion Hs-CRP contributes to diagnosis of CHD,and combined detection of hs-CRP and blood lipids is of important value in evaluating the risk and prognosis of CHD.

OBJECTIVE:To provide theoretical basis for the detection of paracetamol by infrared spectroscopy. METHODS:0.002-0.02 g paracetamol was accurately weighed,added into 0.2 g potassium bromide,respectively,fully ground and well mixed, potassium bromide pressing plate method was used for to-be-tested test sample. Using hollow cathode lamp as light,scanning fre-quency was 30 times,resolution was 0.5 cm-1,scan range was 4000-400 cm-1,infrared absorption spectrum was determined and recorded to select the characteristic absorption peaks,then determine the optimized characteristic absorption peaks. Linear and non-linear models were respectively established by using mathematics modeling methods. RESULTS:1 016 cm-1 absorption peak with weaker absorbance but stronger features was selected for the analysis. According to the model establishing and calculation,the accu-racy of the nonlinear model was much higher than the linear model,r=0.942. CONCLUSIONS:Nonlinear quantitative model for quantitatively determining the content of paracetamol is feasible,and suitable for the on-destructive and rapid on-line quality control of paracetamol.

OBJECTIVE: To investigate the clinical efficacy of nasal cellulose powder for the treatment of allergic rhinitis. METHOD: Thirty-six cases of patients with allergic rhinitis were randomly divided into control group and experimental group, 18 cases in each group. The control group was treated with physiological sea water and the experimental group with nasal fibrous powder. In 14, 28 days after drug evaluation in patients objective and subjective symptoms and signs improved nasal function (nasal airway resistance, the sense of smell) was compared. RESULT: The experimental group and the control group of subjective symptoms and objective nasal function were improved, but the experimental group was significantly higher than that of control group, the difference was statistically significant (P<0.05); Two groups of patients had no adverse reaction occurred. CONCLUSION The clinical curative effect of Nasal cellulose powder used in the treatment of allergic rhinitis is distinct, without adverse reactions, and is conducive to improving patient stuffy nose, nasal itching, sneezing and other symptoms and improve the patients quality of life, is worth clinical use.
Administration, Intranasal ; Cellulose ; therapeutic use ; Humans ; Quality of Life ; Rhinitis, Allergic ; drug therapy ; Treatment Outcome

Objective To establish a HPLC method for determination of benzylacetone in the rhododendron.Methods The HPLC analysis was carried out on Luna C18(250 mm×4.6 mm,5 μm) at 30℃temperature.The mobile phase was acetonitrile-water (64 ∶ 36),the flow rate was 1 ml/min,and the detection wavelength was 220 nm.Results The linear range of benzylacetone was 17.08～170.8 μg/ml (R=0.9998),the average recovery of this method was 99.81％,and the RSD was 1.46％.Conclusion The method is convenient,quickly and accurate,can be used for the quality control of rhododendron oil and their preparations.

Objective To explore the cognitive status of community-dwelling older adults with subjective memory complaints (SMC).Methods A total of 262 older adults with SMC were included based on convenience sampling.Face-to-face interview was conducted for demographic information collection and cognitive assessment.Statistical analyses Were undertaken using SPSS version 19.0.Results The participants with SMC had a low level of general cognitive function,with a mean score of 22.18±4.21 on the MoCA; the two cognitive domains of abstraction' and deIayed memory had the lowest scores,with an average score of 0.85±0.82 and 2.91± 1.50 respectively,and the average scoring rate lower than 60.00％.About 54.20％ of the older adults with SMC presented with cognitive impairment.The scores ofabstraction were low in the participants with and without cognitive impairment.In addition,the scores of visuospatial/executive function, language and delayed memory were low in the participants with cognitive impairment.The cognitive impairment of persons with SMC may be associated with age and marital status.The people over the age of 70 years and widowed ones are more likely to suffer from cognitive dysfunction.Conclusion The older adults with SMC have a low level of general cognitive function,and cognitive imPairment detection rate is higher.Advanced age and widowhood are important factors affecting their cognitive function.

Objective To explore the protective effect of ozone oxidative preconditioning on hepatic ischemia reperfusion injury in rats.Methods Eighteen 8-week-old SPF Sprague-Dawley male rats weighting 250 ～300 g were randomly divided into three groups (n =6 each):sham operation group (group S),ischemia/reperfusion group (group I/R) and ozone oxidative preconditioning group (group O3 + I/R).In Group O3 + I/R,rats received five-day preconditioning treatments by intraperitoneal injection of ozone and oxygen mixed gases (ozone concentration 50 mg/L,1 mg · kg-1 · d-1),and then experienced the procedure of hepatic ischemia/reperfusion injury.Model of hepatic ischemia/reperfusion injury was established by clamping the branches of hepatic artery and portal vein in the median and left lateral hepatic lobes for 45 min,followed by 3-h reperfusion.After reperfusion,blood samples were taken from the aorta abdominalis for detecting serum aminotransferases (ALT & AST).These rats were executed and the hepatic tissue samples were collected for measuring hepatic malondialdehyde (MDA) and superoxide dismutase (SOD) level.Results Compared with group S,concentrations of serum ALT,AST and hepatic MDA were increased in group I/R and O3 + I/R;concentrations of hepatic SOD were decreased (P ＜ 0.05) in group I/R,but concentration of hepatic SOD was increased in group O3 + I/R.Compared with group IR,concentrations of serum ALT,AST and hepatic MDA were decreased,while concentration of hepatic SOD was increased in group O3 + I/R (P ＜ 0.05).Conclusion Ozone oxidative preconditioning could inhibit the lipid peroxidation to protect the rats against hepatic ischemia/reperfusion injury.

The aim of the present study was to explore the prevalence characteristics of brucellosis in Hainan by investigating the biological characteristics and clinical type of pathogenic bacteria isolated from the blood of a patient infected by infective endocarditis.Bacteriological experiments were conducted according to the standard identification methods of Brucella including morphology,cultural characters,biochemistry characters,serological test and phage test,etc.At the same time,systematic analysis on the information about epidemiology,clinical manifestation and laboratory data of the patient was carried on.The Brucella melitensis was identified as Brucella melitensis biotype 2 and was significantly different from the Brucella melitensis biotypes 1 and 3 isolated in China reported in recent years.The urease test result of Brucella melitensis was variable and there is no strong positive result reported except the isolates in Hainan.As Brucellosis cause by Brucella melitensis biotype 2 was firstly reported in Hainan Province,great importance should be attached to its point-like prevalence.

BACKGROUND:Oligo(poly(ethylene glycol)fumarate) (OPF) hydrogel is a kind of biomaterial with good biocompatibility, injectability, and biodegradability. It is known that there are differences in the characteristics of hydrogels with different molecular weights. Under osteogenic induction, bone marrow mesenchymal stem cels encapsulated in the suitable molecular weight hydrogel have better proliferation and differentiation. Therefore, the use of OPF hydrogel provides new options for bone tissue-engineered scaffold.
OBJECTIVE:To investigate the effect of OPF hydrogel with different molecular weights on the proliferation and differentiation of encapsulated rat bone marrow mesenchymal stem celsin vitro.
METHODS: Four kinds of OPF hydrogels with molecular weights of 1 000, 3 000, 10 000 and 35 000 were developed by OPF crosslinking with a redox radical initiation system. Hydrogel sweling and degradation properties were detected. Afterwards, bone marrow mesenchymal stem cels encapsulated in different hydrogels were cultured in osteogenic medium for 1-3 weeks. The effects of hydrogels on morphplogy of bone marrow mesenchymal stem cels and osteogenic differentiation were detectedvia histological staining (hematoxylin-eosin staining and alizarin red staining) and immunofluorescence staining.
RESULTS AND CONCLUSION: With the increase of molecular weight of hydrogels, the gelation time was shortened, the sweling ratio significantly increased, and the hydrogel degradation rate was proportional to the molecular weight. In addition, the number of mineralized nodules formed in 3 000 and 10 000 molecular weight hydrogels was more than that in the other hydrogels, indicating that hydrogels with appropriate sweling and degradation properties are beneficial to cel proliferation and differentiation. In conclusion, OPF hydrogels have good biocompatibility and hydrogels with 3 000 and 10 000 molecular weight play active regulatory roles in osteogenic differentiation of mesenchymal stem cels.

Objective To evaluate the effect of discharge planning service mode in patients with intracoronary stent implantation. Methods Total?ly 106 patients with intracoronary stent implantation were divided into intervention group and control group,the intervention group received discharge planning services. The control group received routine nursing and follow?up of Department of Cardiology. The intervention lasted 6 months. Compari?son of two groups of patients in hospitalization days,cost of hospitalization and cardiovascular events and the rate of readmission and Compliance be?havior and clinical indicators and quality of life. Results The incidence rate of cardiovascular events and readmission rate in discharge planning group is lower than the routine nursing group(P<0.05);Discharge planning group of smoking cessation rates,weekly rehabilitation exercise times and coronary heart disease in two level prevention drug use rate is superior to that of routine group(P<0.05). Discharge planning group,left ventric?ular ejection fraction,success rate of blood lipids and the quality of life is better than the routine group(P<0.05). Conclusion Discharge plan?ning intervention can reduce the incidence of cardiovascular events and readmission rate,improve ventricular function and the compliance behavior and the quality of life in patients with coronary stent implantation.

Objective To evaluate the effect of multidisciplinary intervention on antimicrobial prophylaxis in neurosurgical patients undergoing craniotomy for tumor resection.Methods By standardizing preoperative disinfection and surgical procedures, training and education of antimicrobial use, conducting quality control activities, and performing periodic feedback of supervised results, efficacy of improvement of antimicrobial prophylaxis in neurosurgical patients undergoing craniotomy for tumor resection in a hospital was observed.Results In 2013 and 2014, incidences of surgical site infection(SSI) were 7.66%(17/222)and 6.80%(27/397) respectively,there was no significant difference between two groups(X2 =0.158,P>0.05).Prophylactic use of antimicrobial agents in surgical patients were as follows: cefuroxime(n=495), cefazolin(n=103), cefathiamidine(n=9), clindamycin(n=8), and cefotaxime(n=4), types of used antimicrobial agents were rational.In 2014, the implementation rate of necessary intra-operative antimicrobial adding was 95.02%, which was higher than 87.66% in 2013(X2=7.969,P<0.05), 30.73% of patients received antimicrobial prophylaxis for>48 hours, which was lower than 57.66% in 2013, difference was significant(X2=42.88,P<0.05).Conclusion Multi-intervention measures, including standard preoperative disinfection and surgical procedures, education and training, quality control circle, and feedback of supervised results can improve standard antimicrobial prophylaxis in neurosurgical patients undergoing craniotomy for tumor resection.