Objective:To analyze the edentulous elderly patients with self-locking tracheal intubation fixer and effective improvement of fixed.Methods: From December 2013 to November 2014 in our hospital treated by oral tracheal intubation in elderly patients with a total of 74 cases, all patients according to the fixed mode of tracheal intubation were divided into control group and observation group, compared the two groups of patients with tracheal shift and satisfaction degree, degree of comfort and unexpected accidents.Results: The observation group patients with intubation without shifting the proportion was significantly higher than the control group, while the proportion of moderate displacement was significantly less than the control group, the differences were statistically significant; the observation group patients feel comfortable proportion was significantly greater than the control group, the proportion of discomfort was significantly higher than the control group, the differences were statistically significant; patients in observation group were fixed number was significantly less than the control group, patients satisfaction rate were significantly higher than those in control group, the differences were statistically significant (x2=5.845,P<0.05). Conclusion: The self-locking fixer for tracheal intubation can maximize the comfort degree of patients, improve the operation convenience, reduce the tracheal shift, it is worthy of clinical application.

Objective: To study the acute myocardial infarction (Acute myocardial infarction, AMI) rescue emergency care patient outcomes analysis process optimization (Emergency care process optimization, ECPO). Methods: From July 2012 to May 2013, our hospital 98 cases of AMI patients as research subjects, both ST-segment elevation and emergency hospital. Patients included in the control group (47 cases) before this, the patient is classified after the cut-off point in the observation group (51 cases). The control group received routine emergency care programs, and the observation group ECPO measures to rescue the effect of index contrast two levels, two-hospital AMI recurrence rate and re- PCI hospital treatment ratio, and two groups of patients satisfaction with care process for the situation. Results: The expansion of time and out of the emergency room observation group and emergency balloon time was significantly less than the control group, the difference was statistically significant(t=3.577, t=2.928; P<0.05). AMI hospital observation group relapse rate and re-PCI hospital treatment ratios were significantly less than the control group, the difference was statistically significant(x2=4.006, x2=4.581; P<0.05). The patients' satisfaction for the care process was significantly higher, the difference was statistically significant(x2=11.055, P<0.05). Conclusion: In patients with AMI ECPO rescue measures, the effect is very significant, and can effectively shorten the rescue time and increase the success rate, it is recommended clinical applications.

The concentration of the cyclic nucleotides and its relation with the E-RFC were determined by using ginseng rhizome saponin in human peripharal leukocytes.Result were compared with the ckange of the E-RFC by using cAMP.cGMP and theophylline. Experiments proved that girseng rhizome saponin mainly affected the lymphocytes and significantly increased the concentration of cAMP and cGMP,especially,the cAMP. Lymphocytes were incubated with ginseng rhizome saponin to form Et-RFC. It is different where lymphocytes were incubated in diffierent times. cAMP and theophylline reduced the Ea-RFC and Et-RFC, cGMP increased the Ea— RFC,but no effect to the Et-RFC. Ginseng rhizome or root saponin could promote the Ea-RFC,but inhibited the Et-RFC. It is consistent with the effect of both cAMP and cGMP. Experiments showed that ginseng rhizome saponin increased the concentration of cAMP and cGMP in human lyphocytes followed by that of E-RFC.It was suggested that the change of cyclic nucleotides in lymphocytes may be a mechanism of adjusting the immunity on cell level.

Objective To establish a new kinetic fluorimetry method for the determination of trace nickel in human hair samples. Methods The method was based on the catalysis of nickel on the oxidation of fluorescein(CF) by hydrogen peroxide in an almost neutral medium. As nickel is present, the fluorescence intensity of the solution decreases. Results The wavelength of excitation was 492 nm and the wavelength of emission was 516 nm. The reaction was optimized at 100 ℃ for 10 min. The linear range of determination was 4.0-80.0 ?g/L and the detection limit was 2.2 ?g/L . The RSDs and recovery rates of the method were 2.6%-3.2%, and 96.7%-102.8% respectively. Conclusion This method is simple, rapid and sensitive for the determination of trace nickel in human hair samples.

Objective To establish a new kinetic fluorimetry method for the determination of trace catechol in waste water. Methods Trace catechol inhibited the catalytic oxidation of pyronine Y by potassium periodate in the presence of vanadium(the catalyst) in H2SO4 medium. A linear relation appeared between the concentration of catachol and the changing of catalyzed reaction speed. A new inhibitory kinetic fluorimetry method for the determination of trace catechol in waste water was established. Results The linear range of determination was 10.0-1 500.0 ?g/L,and the detection limit was 7.8 ?g/L. The RSDs and recovery rates of the method were 2.42%-2.71%,and 98.3%-103.3% respectively. Conclusion This method is simple and sensitive for the determination of trace catechol in waste water with satisfactory results.

The changes of skeletal muscle mitochondral monoamine oxidase (MAO) activity in rats fed with low Se grains from a Keshan-disease area were observed. The rats were killed at the time for feeding 30, 60 and 90 days respectiely. The mitochondrium of skeletal muscle was isolated MAO activity was determined with fluorescent methed. The results showed that in low-Se group the mitochondrial MAO activity was significantly decreased only at the time for 90 days feeding however GSH-px activity in red blood cells and Se content in plasma were significantly decreased at all experimental period as compared to Se-supplemented or stock died group.

Sprague—dawey rats were fed for one to three months with Se—deficient grains from Keshan disease area, Se—supplemented diet and stock diet,respectively. Changes of Na~+ ,K~+ ATPase and 5'—nucleotidase activities in cardiac sarcolemma ,Se Contents in liver and plasma and GSH—px activities in Liver and red blood cells of rats were observed. The results showed that Na~+ ,K~+—ATPase. and 5'—nucleotidase activities in cardiac sarcolemma in Se-deficient group rats were significantly decreased,hepatic and plasma Se contents as well as hepatic and erythrocyte GSH—px activities were also significantly decreased at of feeding 30,60 and 90 d as compared to Se—supplemented or stock diet group. There was no significant difference in these parameters between Se—supplemented or stock diet groups There was no significant difference in these paramefers between Se—supplemented and stock diet groups。

Objective:To compare the adverse reactions for patients with brain tumor before and after gamma knife treatment, and analyze the application effect about pertinency nursing intervention measures.Methods: From January 2015 to February 2016, 80 patients with brain tumor who were treated by gamma knife were divided in observation group (40 cases, received pertinency nursing) and control group(40 cases, received normal nursing intervention) with random number table. And to compare the nursing effect difference between the two goups.Results: The average intracranial pressure of the two groups was (186.4±34.2)mmH2O before treatment; it was (232.3±41.5)mmH2O after treatment; and the difference was statistical significant (t=7.634,P=7.634). The observation group were lower than control group in epilepsy, scalp rupture and scalp infection rate, and the difference was statistical significant(x2=4.211,x2=5.541,x2=3.914;P<0.05). The average intracranial pressure and recovery time of observation group were (195.3±36.9)mm H2O and (13.4±3.2)d, respectively, after treatment. Both of the two indexes were lower than (218.2±42.5) mmH2O and (16.1±3.7)d of control group, and the difference was statistical significant(t=2.573, t=3.491;P<0.05). The observation group were lower than control group in occurrence rates of headache, vomiting and papilledema, and the difference were statistical significant (x2=4.114, x2=5.000,x2=4.211;P<0.05); the observation group was higher than control group for treatment satisfaction, and the difference was statistical significant (x2=5.165,P<0.05).Conclusion: The intracranial pressure of brain tumor patients will significantly rise after gamma knife treatment, and the pertinency nursing can reduce the occurrence rates of adverse reaction, intracranial pressure and symptom of encephaledema.

Objective To investigate the pressure sources of caregivers in need of hematopoietic stem cell transplantation (HSCT) and to provide evidence for targeted intervention. Methods According to Hill′s ABC-X family pressure model, 12 main family caregivers were received semi-structured in depth interviews with phenomenological methods. Results Pressure sources of caregivers for HSCT patients were summed up to 7 aspects: search of donor, isolation with patient during transplantation, lack of knowledge in patient care, family resource difficulties, family economic overburden, social role transformation and uncertainty of disease prognosis. Conclusions The pressure sources of caregivers for HSCT patients is a persistent process, which is over layed as the patient′s condition worsens. The analysis on the pressure sources of primary family caregivers is becoming a new topic in HSCT nursing area.

Objective:To compare the immunology parameters and stress hormone in two groups of patients with lung cancer after radical operation and provide reference to clinical surgery.Methods:From March 2015 to March 2016, 72 cases in our hospital were accepted surgical treatment for lung cancer and divided into observation group and control group. Among them 36 cases of video-assisted thoracoscopy lung cancer radical surgery for the treatment of patients in mind as the observation group, 36 cases of open chest lung cancer radical operation were recorded as control group, respectively comparing two groups of patients with preoperative and postoperative 3 days red cell immune index, index of cellular immunity, stress hormone level and blood rheology index.Results:3 days after operation, the observation group in terms of RBC-C3b, RFER levels were significantly higher than those of control group and RBC-IC and RFER were significantly lower than those in the control group. 3 days after operation, the observation group CD3+, CD4+, CD3+CD4+ were significantly higher than those of the control group, while CD8+ was significantly lower than that of the control group. 3 days after operation, the observation group NE, Cor, angiotensin II (ANG II) was significantly higher than that of the control group (t=2.181,t=10.320,t=5.924;P<0.05). 3 days after operation in observation group, the plasma viscosity, erythrocyte sedimentation equation K value were significantly lower than those in the control group. The differences were statistically significant (t=29.698,t=7.411;P<0.05).Conclusion:The side-effect of thoracoscopy radical resection of lung cancer is small, and it has more advantages compared with open chest lung cancer radical operation.