Objective To explore the relationship between the level of synaptic monoamine transmitters and epilepsy and its severity.Methods The models of seizure were made with intraperiloneal injection. The concentrations of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) in palium(Pal), hippocampus (Hip) and corpus and striatum (Str) were determined with flurospectrophotometry. Results The severity of epilepsy was negatively correlated with the level of 5-HT in all three regions (P<0.05) with that of NE in Hip (P<0.01). The level of 5-HT of three seizure groups dropped significantly, the level of DA dropped significantly only in Str (P<0.01), and the level of NE dropped significantly only in Hip (P<0.01). Conclusion Monoamine transmitters in different regions closely affect the severity and sensitivity of epilepsy depends on the level of special transmitters in different brain regions.