1.Post-stroke dysphagia treated with acupoint injection combined with neural electrical stimulation.
Fei-Xiang MA ; Gui-Ping CAO ; Wan-Lang LI
Chinese Acupuncture & Moxibustion 2014;34(12):1169-1173
OBJECTIVETo observe the effect differences on post-stroke dysphagia among acupoint injection combined with neural electrical stimulation, acupoint injection, neural electrical stimulation, and swallowing training respectively, so eligible intervention can be applied to this kind of disease.
METHODSOne hundred and eight-three patients of post-stroke dysphagia were randomized into a comprehensive treatment group (42 cases), an acupoint injection group (44 cases), a neural electrical stimulation group (49 cases) and a swallow training group (48 cases) and were treated with the comprehensive therapy of acupoint injection and neural electrical stimulation, acupoint injection, neural electrical stimulation and swallowing training separately. The treatments for 10 days made one session. There were 3 days at the interval among treatment sessions and 3 sessions were required totally. The cases in those treatment groups were blankly controlled with the other 47 patients of post-stroke dysphagia. All the patients received basic rehabilitation treatment. The modified water swallowing test was conducted to assess the efficacy before treatment, 10 days after treatment and 30 days after treatment in each group separately. The clinical efficacy, score of water swallowing test and improvement in water swallow test were compared among the groups.
RESULTSAfter 10-day treatment, the differences in efficacy and score of water swallow test were not significant in each group (all P > 0.05). After 30-day treatment, the effective rate (94.29%, 33/35) in the comprehensive treatment group was apparently better than 68.75% (22/32) in the acupoint injection group, 80.00% (32/40) in the neural electrical stimulation group, 67.50% (27/40) in the swallowing training group and 42.86% (12/28) in the blank group separately. The score in water swallow test in the comprehensive treatment group was lower than that in each of the other groups (1.37 ± 0.60 vs 2.03 ± 1.00, 1.90 ± 0.90, 2.20 ± 0.72, 2.71 ± 0.90, all P < 0.05). The differences in the effective rate and score in water swallow test were not significant among the acupoint injection group, neural electrical stimulation group and swallowing training group (all P > 0.05), which indicated that the improvement in swallowing function in the comprehensive treatment group was significantly superior to the other groups (all P < 0.05).
CONCLUSIONThe comprehensive therapy of acupoint injection and neural electrical stimulation achieves the much better efficacy on post-stroke dysphagia.
Acupuncture Points ; Adult ; Aged ; Combined Modality Therapy ; Deglutition ; Deglutition Disorders ; drug therapy ; etiology ; physiopathology ; therapy ; Electric Stimulation Therapy ; Female ; Humans ; Male ; Middle Aged ; Stroke ; complications ; Treatment Outcome ; Vitamin B 12 ; administration & dosage ; Young Adult
3.A novel HIF-1 inhibitor--manassantin A derivative LXY6099 inhibits tumor growth.
Fang-Fang LAI ; Xiao-Yu LIU ; Fei NIU ; Li-Wei LANG ; Ping XIE ; Xiao-Guang CHEN
Acta Pharmaceutica Sinica 2014;49(5):622-626
Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor on hypoxia responses in mammalian tissues. HIF-1 plays as a positive factor in solid tumor and leads to hypoxia-driven responses that enhance its downstream gene expression for tumor growth and survival. LXY6099 was obtained by the structural modification and optimization of manassantin A (MA) as a high potent HIF-1 inhibitor. Antitumor activity of LXY6099 was observed in this study. LXY6099 with an IC50 value of 2.46 x 10(-10) mol x L(-1) showed more sensitive inhibition activity to HIF-1 than that of MA detected by reporter gene assay (> 100 folds). It showed strong inhibition on the growth of human solid tumor cell lines. Furthermore, LXY6099 exhibited significant antitumor activity against established human tumor xenografts in nu/nu mice with treatment of MX-1 breast cancer. Thus, LXY6099 as a novel HIF-1 inhibitor could be further developed into anti-cancer agents.
Animals
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Antineoplastic Agents
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pharmacology
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Breast Neoplasms
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metabolism
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Cell Line, Tumor
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Gene Expression Regulation, Neoplastic
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Heterografts
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Humans
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Hypoxia-Inducible Factor 1
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metabolism
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Lignans
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pharmacology
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Mice, Nude
4.The expression and role of BAMBI in pancreatic cancer
Ping WANG ; Haibo LANG ; Xinjuan LIU ; Xue LI ; Hua FAN ; Qiang HE ; Jie ZHANG
Chinese Journal of Pancreatology 2015;15(2):78-80
Objective To investigate the expression of BAMBI in pancreatic carcinoma tissue and its clinical value.Methods Expression of BAMBI was detected by immunohistochemistry in 69 cases of pancreatic cancer and 15 normal pancreatic samples.The survival and its association with clinicopathologic parameters were analyzed.Results The expression of BAMBI in the cancer tissue was much higher than that in the normal tissues (56.5% vs 16.7%,P <0.01).The expression of BAMBI was not associated with age,sex and tumor size,differentiation,but expression of BAMBI in patient with lymph node metastasis was higher than that in patient without lymph node metastasis (67.4% vs 38.5%,P =0.019),the expression of BAMBI in TNM stage Ⅲ was higher than that in stage Ⅰ + Ⅱ (75.0% vs 31.0%,P =0.001).The median survival of BAMBI-positive group (6 months) was lower than that of BAMBI-negative group (10 months).Conclusions BAMBI is over-expressed in pancreatic carcinoma,and its expression is associated with lymph node metastasis,TNM staging,differentiation and prognosis.
5.Clinical research on the therapeutic effects and safety of amitrine bismesylate in patients with mild vascular dementia
Jian-Ping JIA ; Xin WANG ; Sen-Yang LANG ; Zhong-Xin ZHAO ; Xiao-Ping PAN ; Li-Juan WANG ;
Chinese Journal of Neurology 2001;0(03):-
Objective To evaluate the therapeutic effects and safety of amitrine bismesylate in patients with mild vascular dementia.Methods An open multicenter self-controlled trial was carried out with 128 mild vascular dementia patients clinically diagnosed.Patients were treated with amitrine bismesylate in a dose of 2 tablets per day for 12 weeks.The neuro-psychologieal scale of MMSE,ADAS-cog,CDR, ADL were used to evaluate patients′cognitive condition before therapy and 6,12 weeks after treatment.The adverse effects,such as nausea and vertigo and so on,were monitored at the same time to evaluate the safety of this drug.Results After 3 months of treatment,the MMSE score(16.98 before therapy and 17.97 after treatment,P
6.Correction of secondary nasal deformity of cleft lip with autogenous costal cartilage framework.
Jiang LI ; Xiao-Ping GUO ; Ke-Hua WANG ; Dong-Hong ZHAO ; Tong HAN ; Yu-Hong LANG ; Li-Jun PONG
Chinese Journal of Plastic Surgery 2013;29(3):167-169
OBJECTIVETo investigate the correction of secondary nasal deformity of cleft lip with autogenous costal cartilage framework.
METHODS237 cases with secondary nasal deformity of unilateral cleft lip were treated. The rib cartilage was harvested through a mini-invasive incision, and was fabricated as a C-shaped framework, as well as some cartilage fragments. Through transcolumella incision, the C-shaped framework was implanted to support the depressed alar and the cartilage fragments were used to augment the nasal base.
RESULTSSatisfactory cosmetic and functional results were achieved in all the patients with primary healing. 93 patients were followed up one year after operation with good cosmetic results.
CONCLUSIONSAutogenous costal cartilage framework can be used for the correction of secondary nasal deformity of cleft lip with satisfactory results.
Cleft Lip ; surgery ; Costal Cartilage ; transplantation ; Humans ; Nose Deformities, Acquired ; surgery ; Rhinoplasty ; methods
7.Lack of association between the TIGR gene mutation and the high myopia in Chinese children
Ping, WANG ; Zhi-Chun, YE ; Li-Juan, TAO ; Xi-Rong, GAO ; Li-Hua, XIE ; Hui-Ling, YANG ; Xi-Lang, WANG
International Eye Science 2011;11(2):210-213
AIM: To screen TIGR/myocilin gene (MYOC) mutation in high myopic Chinese children with family history.METHODS: Gene sequencing was performed in exon 3 of the TIGR gene in high myopic Chinese Children. The coding sequence of TIGR exon 3 was screened by capillary electrophoresis sequencing. The sequence alterations were analyzed by bioinformatics.RESULTS: TIGR gene mutation was not found in high myopic patients and normal controls group.CONCLUSION: No identified gene mutation is found in TIGR gene in high myopic Chinese children.
8.A genetic diversity comparison between captive individuals and wild individuals of Elliot's Pheasant (Syrmaticus ellioti) using mitochondrial DNA.
Ping-Ping JIANG ; Qiu-Lei LANG ; Sheng-Guo FANG ; Ping DING ; Li-Ming CHEN
Journal of Zhejiang University. Science. B 2005;6(5):413-417
Maintaining genetic diversity is a major issue in conservation biology. In this study, we demonstrate the differences of genetic diversity levels between wild and captive individuals of Elliot's Pheasant Syrmaticus ellioti. Wild individuals showed a higher genetic diversity level than that of the captive individuals. Nucleotide diversity and haplotype diversity of wild individuals were 0.00628 and 0.993, while those of captive individuals were 0.00150 and 0.584 respectively. Only 3 haplotypes of mtDNA control region sequence were identified among 36 captive individuals, while 16 unique haplotypes were identified among the 17 wild individuals in this study. One captive haplotype was shared by a wild individual from Anhui Province. It is concluded that a low number of founders was the likely reason for the lower level genetic diversity of the captive group. Careful genetic management is suggested for captive populations, particularly of such an endangered species, to maintain genetic variability levels.
Animals
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Animals, Wild
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genetics
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Animals, Zoo
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genetics
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Base Sequence
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DNA, Mitochondrial
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genetics
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Galliformes
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genetics
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Genetic Variation
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genetics
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Haplotypes
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Molecular Sequence Data
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Polymorphism, Genetic
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genetics
9.Compare three methods to detect the Pneumocystis carinii in the bronchoalveolar wash sample of AIDS patients.
Liang ZHANG ; Xing-wang LI ; Bing SHEN ; Xiao-ying TENG ; Lei SUN ; Zhen-wei LANG ; Ping YANG ; Peng WANG
Chinese Journal of Pathology 2011;40(7):482-484
AIDS-Related Opportunistic Infections
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diagnosis
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microbiology
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Acquired Immunodeficiency Syndrome
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diagnosis
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microbiology
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Bronchoalveolar Lavage Fluid
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microbiology
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Female
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Humans
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Immunohistochemistry
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Male
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Methenamine
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Pneumocystis carinii
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isolation & purification
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Pneumonia, Pneumocystis
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diagnosis
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microbiology
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Polymerase Chain Reaction
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Silver Staining
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methods
10.Expressions of folkhead helix transcription factor 3 on CD4+ CD25+ regulatory T lymphocyte in intestinal mucosa in human immunodeficiency virus infected patients
Lei SUN ; Zhenwei LANG ; Peng WANG ; Ping LI ; Hongxin ZHAO ; Xiaoying TENG ; Xingang ZHOU ; Liang ZHANG ; Bing SHEN ; Mengdong LAN
Chinese Journal of Infectious Diseases 2011;29(5):300-303
Objective To investigate the changes of CD4+ CD25+ regulatory T lymphocyte (Treg) and expressions of folkhead helix transcription factor 3 (FoxP3) in intestinal mucosa in human immunodeficiency virus (HIV) infected patients. Methods Twenty-one HIV infected patients and 17 control subjects without HIV infection were included in this study. The expression of FoxP3, which was considered as a specific marker of CD4+ CD25 + Treg, was detected in intestinal mucosa specimens from HIV infected patients by immunohistochemistry. Meanwhile, the in situ expression of CD4+ T lymphocyte was also determined by immunohistochemistry. The data were analyzed by t test. Results The positive labeling index of CD4+ T lymphocyte in intestinal mucosa was significantly lower in HIV infected patients compared to the controls (11. 56%±4. 44% vs 43. 49% ±8. 90% ,t=-11. 86,P<0. 01). The positive labeling index of FoxP3 in intestinal mucosa was also significantly lower in HIV infected patients compared to the controls (0.46% ± 0.20% vs 1. 18% ± 0. 44% ,t= - 5. 98,P<0.01). Conclusion The depletion of CD4+ CD25+ Treg is accompanied with the depletion of CD4 + T lymphocyte and the reduction of FoxP3 expression in intestinal mucosa of HIV infected patients.