Small cell lung cancer(SCLC) is an chemosensitive and radiosensitive malignant tumor which can appear hematogenous metastasis in early stage. The treatment of SCLC is based on chemotherapy and combined with radiotherapy and operation. In spite of the high response rate, the median time from drug-resistance to death of extensive SCLC is still disappointed. As to limited SCLC, there are still 75 % ~ 80 % patients relapse after inductive chemotherapy and radiotherapy. All in all, the second-line therapy is very important in SCLC patients.

The respective sense and antisense oligodeoxynucleotides and their phosphorothiods, targeting at the bcr/abl, were the hallmark gene of Chronic Myelogenous Leukemia( CML) and c-myb correlates with CML. K562 cells, derived initially from a patient of CML blast crisis, were incubated with bcr/abl antisense oligodeoxynucleotides asODN or c-myb asODN or with both asODN in combinalion. All kinds of asODN could not only significantly inhibit K562 cells survival with the highest inhibitory rate of 64.7%?3.2%, but also dramatically inhibit DNA synthesis and the highest inhibitory rate was 85. 8 %?4.1%, decrease bcr/abl mRNA level almost completely and induce apoptosis significantly. The inhibition effect of antisense oligodeoxynucleotides phosphorothiodes s-asODN was stronger than that of unmodified asODN. The inhibition of the combination of bcr/abl and c-myb asODN was stronger than that of anyone alnoe. All the inhibitions exhibited in sequence-and time-dependent manner. We concluded that bcr/abl in pathogenesis of CML not only increased CML cells proliferation rate, but also decreased CML cells apoptosis rate. c-myb may participate in CML mainly by regulating bcr/abl. Trie results indicated that the modified asODN and multiple asODN targeting several oncogenes may advance to antisense gene therapy.

Objective To study the imaging characteristics of breast fibroadenoma on mammograms.Methods Mammograms of 81 cases with breast fibroadenoma confirmed by operation and pathology were reviewed retrospectively. Results Fifty-four cases had isodense masses;11 cases with highdense masses;13 cases with phyllodes masses.“Membranoid sign” can be seen in 44 cases,19 cases with indistinct margin,6 cases with benign calcifications. Accurate preoperative diagnoses were made in 65 cases. Eight cases were misdiagnosed as breast carcinoma or adenosis respectively.The diagnosis of “no malignant lesions” was made in 8 cases.Conclusion The recognition of typical and atypical X-ray findings of breast fibroaderoma is very important,mammography is a good methods in detecting and differentiating breast diseases.

Objective To explore the effect of calcium gene-related peptide (CGRP) on free calcium concentration in the brain cells of hypoxic-ischemic neonatal SD rats. Methods Animal model of hypoxic-ischemic cerebral injury was set up using SD rats of 7 days old. Then the rats were randomly divided into treatment group given 3?g/kg/d CGRP intraperitoneally for 3 days immediately after the model was made, and salt solution group given 0.9% NaCl solution intraperitoneally for 3 days. Normal control group received sham operation. All the rats were decapitated after 3 days and the concentration of free calcium in brain cells was measured with calcium fluorescence indicator in Fura-2Am. Results The free calcium concentration in brain cells in salt solution group was significantly higher than that in the other two groups (P

Objective To investigate the common allergens and the relationship between the common allergens and the age of children with bronchial asthma in Jiading area of Shanghai. Methods Skin prick test (SPT) of 15 common allergens with standard prick liquid were performed in 351 asthmatic children, the children were divided into ≤3 years group, 3 ～6 years group and ＞6 years group, and we observed the positive rates and allergens in different age groups. Results (1) The positive rate of SPT for inhalant allergens in children with bronchial asthma is 71. 2%, specifically are: dust mite (49. 6%), house dust mite (49. 0%), fungi Ⅰ (36. 8%), mold Ⅱ (34. 8%), tree Ⅰ (32. 5%), dog epithelium (31.9%), cat epithelium (31.3%), pollen (29. 1%), tree Ⅱ (28. 8%), and feather (27.4%). (2)The positive rate of SPT for food allergens in children with bronchial asthma is 39. 6%, specifically are: shrimp(24.2%), eggs(15.7%), milk(14.8%), peanuts (13. 7%), and curries(12.5%). (3) The positive rate of inhalant allergens was significantly higher than food allergens. The positive rate of inhalant allergens had no significant differences among different ages, while the positive rate of food allergens was increased with age. Conclusions Most children with bronchial asthma and allergens are related, and allergens are related to age.

Objective: To study the effects of exogenous nucleotides on immune function in mice.Methods: Sixty Kunming mice, (20?2) g, 5-6 weeks old were divided into three treatments groups (fed 0,0.05%,0.25% nucleotide-supplemented diet respectively). Each treatment was divided into two groups: normal group and immunodepression group. Mice in immunodepression groups were injected with cyclophosphamide (150 mg/kg bw) on the 2nd day in order to suppress the immune function and the mice in normal group were treated with normal saline at the same time. All mice were injected with SRBC on the 6th day. 0,0.05%,0.25% nucleotides were added into basic diet respectively and fed for 10 days. Body weight gain, organ index (thymus and spleen), SI, antibody anti SRBC and antibody secreting cell number in spleen were measured. Results: The body weight gain(P

Objective: To study the effect of dietary nucleotides on DNA damage of thymocytes in mice. Methods: KM mice (n=30) , 5-6 weeks, were randomized into 3 groups,negative group (group 1), positive group (group 2) and nucleotides group (group 3). Mice in group 1 and 2 were fed nucleotide-free diet and group 3 nucleotide-supplemented diet (0.25% nucleotides). Mice in group 2, 3 were given single cyclophosphamide intraperitoneal injection of 150 mg/kg bw at day 21. DNA damage in thymus lymphocytes was evaluated 18 h after injection by single-cell gel electrophoresis (SCGE) assay. Thymus and spleen were weighed. Results: Dietary nucleotides had no effect on weights of thymus and spleen, but significantly decreased the number of damaged lymphocytes and the degree of damage. Conclusion: Dietary nucleotides may protect thymocytes DNA in mice from damage.

Objective To study effect of TCM-based syndrome differentiation combined with diet nursing and point massage on insomnia.Methods Five hundred and twelve patients with insomnia were randomized into control group(n=85)and intervention group (n=67).The patients in both groups received treatment by TCM-based syndrome differentiation.Besides,the control group was given conventional nursing while those in the intervention group received diet nursing and point massage.The two groups were compared in three months in terms of sleep quality and curative effect.Results In comparisons of sleep quality index,the two groups both had statistically significant improvements respectively(both P<0.05).In terms of time to sleep, duration of sleep an sleep efficacy and Pittsburg sleep quality index,the intervention group scored significantly lower than the control group(all P<0.05).In terms of curative effect,the intervention group was significantly higher than the control group(P<0.05).Conclusion The method of TCM-based syndrome differentiation combined with diet nursing and point massage is effective in improving the sleep quality of those with insomnia.

Objective To investigate the value of Pituitrin in neonatal cardiopulmonary resuscitation.Methods Seventy-three cases with neonatal cardiopulmonary arrest admitted in emergency department and NICU in our hospital were collected during 2007 to 2011.Newborns who did not respond to conventional neonatal resuscitation therapy were divided into two groups:epinephrine group 47 cases (control group) and Pituitrin combined with epinephrine group 26 cases (treatment group).Results There were no statistical difference (x2 =0.956,P ＞ 0.05) between treatment group and control group in the rates of initial resuscitation success (23.1％,6/26 vs 34.0％,16/47).Conclusion Pituitrin combined with epinephrine has similar efficacy with the use of epinephrine in neonatal cardiopulmonary resuscitation.

Objective To identify the expressions and distributions of aquaporin-1(AQP 1) and microvessel density(MVD) in human cervical carcinomas and their relationship,and investigate the roles of AQP1 and MVD in human cervical carcinomas. Methods The expressions of AQP1 and MVD in 74 cases of cervical carcinoma(46 cases of squamous-cell carcinoma of the uterine cervix,28 cases of in adenocarcinoma of the uterine cervix),in 34 cases of cervical intraepithelial neoplasia(CIN) and in 15 cases of normal cervices by immunohistochemical technique.Results The expression of AQP1 was found in vascular endothelial cell of CIN,squamous-cell carcinoma and adenocarcinoma of the uterine cervix,with the largest amount in adenocarcinoma and a same amount in CIN and squamous-cell carcinoma of the uterine cervix.There was distinct difference in the intensities of squamous-cell carcinoma,adenocarcinoma of the uterine cervix and control groups.The expression rates of MVD gradually increased with the progress of cervical lesion.There were significant differences between the above 4 groups for MVD(P