OBJECTIVETo explore the mechanism of anti-inflammatory effect of mangiferin.

METHODThe model of chronic bronchitis in rat was established by LPS + smoke. The activity of SOD, content of MDA and NO in BALF and serum, content of TNF-alpha and IL-8 were determined. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS in mice was detected by RT-PCR.

RESULTThe activity of SOD, the content of NO in BALF and serum in rat with chronic bronchitis were significantly higher with high, medium and low-dose of lg mangiferin (400,200,100 mg x kg(-1)), while the content of MDA, and the content of TNF-alpha and IL-8 in lung tissues were lower. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS was significantly reduced by mangiferin with 200,100, 50 micromol x L(-1).

CONCLUSIONThe anti-inflammatory mechanism of mangiferin is to relieve inflammation by raising the activity of SOD and content of NO and reducing the content of MDA and the expression of TNF-alpha, IL-8 and COX-2 mRNA.

Animals ; Bronchitis, Chronic ; drug therapy ; genetics ; immunology ; Cell Line ; Cyclooxygenase 2 ; genetics ; immunology ; Cytokines ; genetics ; immunology ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; Gene Expression ; drug effects ; Humans ; Macrophages ; drug effects ; enzymology ; immunology ; Male ; Mice ; Rats ; Rats, Sprague-Dawley ; Xanthones ; administration & dosage ; pharmacology