1.Separation and Preliminary Identification of Spoilage Organisms in Transmutative Soy Milk
Li-Ping WANG ; Qin-Hua ZHANG ; Yong ZHAO ; You-Rong CHEN ; Feng-Lan QI ; Wen ZHANG ;
Microbiology 1992;0(04):-
In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage bacteria could survive condition of both 1?105Pa,30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and Brevibacillus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668。
2.The inhibiting effect of polygoni multiflori total glycosides (PMTG) on the atherosclerotic lesion formation in experimental atherosclerosis.
Wei FANG ; Hui-xin ZHANG ; Lü-ya WANG ; Ying WU ; Yan-wen QIN ; Wei WANG ; Lan-ping DU ; Bin LIU
Chinese Journal of Pathology 2005;34(9):600-601
Animals
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Aorta
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pathology
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Apolipoproteins E
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deficiency
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Atherosclerosis
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metabolism
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pathology
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Female
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Glycosides
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isolation & purification
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pharmacology
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Intercellular Adhesion Molecule-1
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metabolism
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Mice
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Plants, Medicinal
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chemistry
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Polygonum
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chemistry
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Vascular Cell Adhesion Molecule-1
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metabolism
3.Genotypes and serotypes of avian infectious bronchitis viruses isolated during 2009-2011 in Guangxi, China.
Li-Li QIN ; Meng LI ; Rong SUN ; Zhi-Jin WU ; Kun HE ; Mei-Lan MO ; Tian-Chao WEI ; Ping WEI
Chinese Journal of Virology 2014;30(2):162-170
In order to investigate the prevalence and track genetic and antigenic evolutions of infectious bronchitis virus (IBV) and their prevalence in Guangxi, China since 1985, gene amplification and sequencing and virus neutralization (VN) test on chicken embryo tracheal organ cultures were used in genotyping and serotyping of 28 IBV isolates during 2009-2011 in Guangxi. The results of N gene sequencing and comparison showed that the 28 isolates and reference strains were classified into three groups, and most isolates belonged to group Ill, while the isolates in 1985-2008 belonged to groups IV and II. The data of VN test indicated that the 28 isolates belonged to 6 serotypes; among them, 71. 4% belonged to serotypes 1, 2, and 3, and 11 (39.3%) shared the same serotype with the current vaccine strains. Given the data of our previous study, it is found that prevalent serotypes and their proportions varied in different areas of Guangxi and during different periods. These data lay a good foundation for developing an oil-emulsified inactivated polyvalent vaccine containing local dominant serotypes for the effective prevention and control of infectious bronchitis.
Animals
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Antibodies, Viral
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immunology
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Chick Embryo
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Chickens
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China
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epidemiology
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Coronavirus Infections
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epidemiology
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immunology
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veterinary
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virology
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Infectious bronchitis virus
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classification
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genetics
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immunology
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isolation & purification
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Molecular Sequence Data
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Phylogeny
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Poultry Diseases
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epidemiology
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immunology
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virology
4.Association Between Plasma Fibrinogen Concentration and Ten-year Accumulative Risk of Essential Hypertension
Jun CHENG ; Dong ZHAO ; Wei WANG ; Jia-Yi SUN ; Yan LI ; Lan-Ping QIN ; Zhao-Su WU ;
Chinese Journal of Hypertension 2007;0(04):-
Background Studies showed plasma fibrinogen (FIB) is not only the independent risk factor of cardiovascular disease,but also associated with the prevalence and incidenc of hypertension.A gender difference was shown in the association of FIB with hypertension,while no epidemiological study has been reported in Chinese population.Objective To explore the association between plasma FIB concentration and ten-year accumulative risk of hypertension.Methods A prospective study of relationship between blood pressure and other traditional risk factors were carried out in a cohort of Chinese population of 2115 people aged 35-64 years old in 1992 and 2002. The association of plasma FIB concentration with ten-year accumulative risk of hypertension was analyzed.Results The ten-year accumulative incidence rate of hypertension was 34.8%,and incidence in males (39.3%) was signifi- cantly higher than that in females (31.5%) (P
5.Analysis of 89 amniotic samples using fluorescent in situ hybridization.
Ping WEI ; Yun-xing LI ; Lan ZENG ; Chun CHEN ; Sheng-fang QIN ; Xue-yan WANG ; Na XI ; Shu-qin TANG ; Mei LENG ; Chun-lan LIU
Chinese Journal of Medical Genetics 2013;30(2):214-217
OBJECTIVETo assess the value of fluorescent in situ hybridization (FISH) for detecting common chromosome aneuploidies in interphase nuclei of amniotic fluid cells.
METHODSEighty two uncultured amniotic fluid samples and supernatants from 2 successfully and 5 unsuccessfully cultured amniotic fluid samples were analyzed with FISH. Results from standard cytogenetic analysis of 79 uncultured amniotic fluid samples and 2 successfully cultured amniotic fluid samples were compared with FISH results.
RESULTSAll of the 89 samples were succeeded analyzed with FISH. Positive findings included 3 cases with trisomy 21, 1 case with 47, XYY and 1 case with 69, XXX, which were consistent with results of karyotype analysis.
CONCLUSIONFISH is a rapid and accurate method for prenatal diagnosis, and can also provide a remedy to failed amniotic fluid cells culture.
Adult ; Amniotic Fluid ; cytology ; Cell Culture Techniques ; Female ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Karyotyping ; Pregnancy
6.Clinical evaluation for sublingual immunotherapy of allergic asthma and atopic rhinitis with Dermatophagoides Farinae Drops.
Lan-Fang CAO ; Quan LU ; Hong-Liang GU ; Yan-Ping CHEN ; Yuan ZHANG ; Min LU ; Yao-Qin QIAN ; Lan LI ; Yi-Ping XU
Chinese Journal of Pediatrics 2007;45(10):736-741
OBJECTIVETo evaluate the safety and efficacy of sublingual immunotherapy with 'Dermatophagoides Farinae Drops' in D. farinae allergic asthma and/or rhinitis patients.
METHODSA 25-week double-blind, placebo-controlled, multi-centered trail was conducted in 278 children (aged 4 - 18 yr) with mite-induced asthma and/or rhinitis. Patients were randomly assigned to receive sublingual immunotherapy (SLIT) with 'Dermatophagoides Farinae Drops' (n = 139) or placebo (n = 139) for 25 weeks and the dosage and administration strictly followed the manufacturer's instructions. At the beginning of the 2nd, 3rd, 4th, 6th, 10th, 14th, 18th, 22nd week of the treatment, the patients were asked to accept follow-up visit, during the clinical trial all patients and parents were asked to keep a daily record of their asthma symptom scores, rescue medicine use, rhinitis symptom scores, morning and evening peak expiratory flow. Asthma symptom scores, reduction in use of rescue medicine, rhinitis symptom scores, lung function tests, skin sensitivity to mite, mite-specific immunoglobulin (Ig) E and IgG4, and quality of life and adverse effect were assessed during the study.
RESULT(1) Of the 278 children, 27 dropped out before the study completion. (2) After 25 weeks of treatment, the median variability of PEFR was -1.38 for SLIT group and -0.90 for the placebo (P < 0.05). (3) Besides, the mean variability of medicine score of asthma was -0.08 for SLIT group and 0.52 for the plcebo (P < 0.05). (4) The median variability of rhinitis symptom score was -1.96 for SLIT group and -1.03 for the placebo (P < 0.01). (5) The rescue medicine usage of SLIT reduced but did not show significant differences between SLIT and placebo. (6) After 25 weeks treatment, the increase of D. farinae specific IgE antibody of two groups were similar, while specific IgG4 increased significantly in SLIT compared to the patients in control one (P < 0.01); (7) No severe adverse events happened in the trial and the most-likely adverse events were mild asthma and local rash.
CONCLUSIONDermatophagoides Farinae Drops is safe and effective in treating allergic asthma and atopic rhinitis.
Adolescent ; Animals ; Asthma ; therapy ; Child ; Child, Preschool ; Dermatophagoides farinae ; chemistry ; metabolism ; Double-Blind Method ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Mites ; chemistry ; Rhinitis ; therapy
7.Establishment of a nude mouse model bearing orthotopically transplanted human ovarian carcinoma expressing green fluorescent protein.
Ai-lan YIN ; Mei ZHONG ; Gui-qin SUN ; Li-ping WANG ; Shan-shan ZHAO
Journal of Southern Medical University 2008;28(3):484-486
OBJECTIVETo establish a human ovarian cancer-bearing mouse model via orthotopic transplantation of human HO-8910 cells expressing green fluorescent protein (GFP).
METHODSGFP-expressing human ovarian carcinoma HO8910/GFP cells (2 x 10(6)) in exponential phase of growth were inoculated subcutaneously in nude mice, and the generated tumor tissues were collected and transplanted below the capsule of the left ovary of 6 nude mice. The growth of the tumors was observed in vivo using a fluorescence stereomicroscope. The nude mice were sacrificed 4 weeks after transplantation to assess the tumor growth and metastasis.
RESULTSThe tumors showed progressive growth at the orthotopic sites in all animals. Two weeks after the transplantation, green fluorescent mass was observed at the left costovertebral angle, and the mass increased thereafter and invaded or metastasized to the peritoneum, omentum, spleen, liver, uterus, and the pelvic lymph nodes, with a metastatic rate as much as 66.7%.
CONCLUSIONThe nude mouse model bearing orthotopic human ovarian carcinoma expressing GFP has been successfully established.
Animals ; Cell Line, Tumor ; Disease Models, Animal ; Female ; Green Fluorescent Proteins ; genetics ; metabolism ; Mice ; Mice, Nude ; Microscopy, Fluorescence ; Neoplasm Transplantation ; Neoplasms, Experimental ; genetics ; metabolism ; pathology ; Ovarian Neoplasms ; genetics ; metabolism ; pathology ; Transplantation, Heterologous
8.A cross-sectional study on cognitive function and influencing factors in patients with hypertension.
Wen-hua WANG ; Dong ZHAO ; Sa LIU ; Lan-ping QIN ; Zhao-su WU
Chinese Journal of Epidemiology 2007;28(6):532-535
OBJECTIVETo compare the differences of cognitive functions in patients with hypertension and normotensives, and to analyze the primary influencing factors on cognitive functions.
METHODSThis was a cross-sectional study carried out in two community populations of Beijing in 2001. The study subjects consisted of 83 hypertensive individuals aged 50-65 years, who were both stroke and dementia-free, the control group was chosen with 83 normotensives who were matched one by one with hypertensive individuals on age, sex, educational level and occupation. Socio-demographic, behavioral, medical history, and physiological data were collected on all participants through interview and medical examination. A comprehensive and computerized neuropsychological battery was administered.
RESULTSThe total score of Basic Cognitive Ability Examination on hypertension (63.62) was worse than that among controls (68.58) with P < 0.01. Mean reaction time of Digit Discrimination of hypertensive (1.25) was longer than controls (1.17) with P < 0.05. The span of Digit Working Memory of hypertensive (4.96) was shorter than controls (5.63) with P < 0.05. The Score of Dual-Word Recognition of hypertensive (12.05) was lower than controls (13.45) with P < 0.01. Educational level, age and hypertension were the primary influencing factors on cognitive function.
CONCLUSIONPatients with hypertension performed significantly worse than controls on velocity of perception, working memory and word memory. The findings suggested that the prevention of hypertension could protect cognitive function.
Aged ; Case-Control Studies ; Cognition ; physiology ; Cross-Sectional Studies ; Female ; Humans ; Hypertension ; physiopathology ; Linear Models ; Male ; Middle Aged ; Neuropsychological Tests
9.Study of molecular mechanism of tanshinone II A inducing differentiation in acute promyelocytic leukemia NB4 cells.
Rui DU ; Hong ZHENG ; Yan-Ping WANG ; Wen-Tong MENG ; Hui QIN ; Shu-Lan YUAN
China Journal of Chinese Materia Medica 2008;33(24):2954-2958
OBJECTIVETo investigate molecular mechanism of tanshinone II A inducing differentiation and apoptosis in acute promyelocytic leukemia NB4 cells.
METHODNB4 cells were cultured in vitro and treated with tanshinone II A and observed cellular morphology, cell category and the cellular proliferation. DNA microarray technique was used to analyze the gene expression profiles of NB4 cells induced by tanshinone II A.
RESULT92.8% of NB4 cells treated with 0.5 mg x L(-1) tanshinone II A were induced into mature neutrophils, in which myetocytes and melamyetocytes were 27.0%, banded and segmented neutrophits 68.2%. Cell growth were inhibited. cDNA microarray showed the enormously expressed 183 genes including 23 differentiation associated genes, and other interrelated genes.
CONCLUSIONTanshinone II A inducing differentiation in NB4 cells may be via regulation of many kinds of genes, especially differentiation associated genes expression. This partially explained the molecular mechanism of tanshinone II A inducing differentiation.
Cell Differentiation ; drug effects ; Cell Line, Tumor ; Diterpenes, Abietane ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Regulation, Leukemic ; drug effects ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; genetics ; metabolism ; Phenanthrenes ; pharmacology
10.Preliminary study of 18F-FDG PET-CT in defining lymph node radiation target volume for non-small-cell lung cancer patients.
Lan-Ping LIU ; Jin-Ming YU ; Hong-Bo GUO ; Zheng FU ; An-Qin HAN ; Guo-Ren YANG
Chinese Journal of Oncology 2007;29(6):453-456
OBJECTIVETo investigate the value of 18F-FDG PET-CT in detecting metastatic lymph node and radiation planning for patient with non-small-cell lung cancer (NSCLC).
METHODSThe data of 58 NSCLC patients were retrospectively analyzed. Preoperatively, Both 18F-FDG PET-CT and CT scan were performed for all patients. Blinded interpretation of these images was then carried out. The gross tumor volume defined by radiation oncologist based on imaging results of either PET-CT or CT alone was compared with pathological results eventually.
RESULTSThe sensitivity, specificity, accuracy, positive predictive value and negative predictive value of detecting metastatic lymph node in the mediastinum was 56.0%, 54.2%, 54.8%, 38.9%, 70.3% by CT alone, and 88.0%, 85.4%, 86.3%, 75.9%, 93.2% by PET-CT, respectively. There was a statisticalty significant difference between two methods(P < 0.05). However, statisticalty no significant difference in detecting hilar lymph nodes was observed. The radiation target volume defined by CT was identical to that by PET-CT in 31(53.5%) cases, but changed in the other 27 (46.5%) cases when 18F-FDG PET data was added. The accuracy was 75.9% in detecting metastatic lymph nodes in the radiation target volume defined by PET-CT, while it was much lower by CT alone (48.3%) with a statistically significant difference between two methods (P < 0.05).
CONCLUSION18F-FDG PET-CT is more accurate than CT alone in assessment of mediastinal lymph nodes for NSCLC patients. It may be recommended as a method in defining the radiation target volume.
Adult ; Aged ; Aged, 80 and over ; Carcinoma, Non-Small-Cell Lung ; diagnostic imaging ; pathology ; radiotherapy ; Female ; Fluorodeoxyglucose F18 ; Humans ; Lung Neoplasms ; diagnostic imaging ; pathology ; radiotherapy ; Lymph Nodes ; diagnostic imaging ; pathology ; radiation effects ; Lymphatic Metastasis ; Male ; Mediastinum ; diagnostic imaging ; radiation effects ; Middle Aged ; Positron-Emission Tomography ; methods ; Radiopharmaceuticals ; Retrospective Studies ; Tomography, X-Ray Computed