In the study, we determined the rate of appearance of micronuclei in lymphocytes of patients with malignant or benign gastric disorders as well as healthy subjects. The values are 3.40?0.26% for healthy subjects, 9.85?0.75% for gastric carcinoma, 5.62?0.33% for atrophic gastritis, and 4.86?0.23% for superficial gastritis (mean?SEM). There are very significant differences between gastric carcinoma and controls(p0.05). The results suggest that the rate reflects the degree of mutation and damage of chromosomes. The number of micronuclei increases with the degree of malignancy of carcinoma, suggesting that the determination of micronuclei may have a prognostic value and predictive of a relapse of carcinoma. The authors suggest that this method may be used as a tool in mass screening of gastric cancers.

Objective To investigate whether the early use of tetramethylpyrazine(TMP) could improve the oxygenation ,reduce the rate of pulmonary lesion ,increase the survival rate and shorten the hospital days in the patients with severe acute pancreatitis (SAP) .Methods A total of 42 patients with SAP were randomly divided into the control (C) group(21 cases ,receiving the conven-tional treatment combined with the respiratory support treatment ) .The TMP group(21 cases ,receing the conventional treatment , respiratory support and TMP treatment ) .Results In the C group ,6 cases were intubated(28 .57% ) ,9 cases were acute respiratory distress syndrome(ARDS)(42 .86% ) ,the survival rate was 80 .95% ,the average hospitalization was (21 .6 ± 7 .3)d;in the TMP group ,3 cases were intubated(14 .28% ) ,6 cases were ARDS(28 .57% ) ,the survival rate was 85 .71% ,the average hospitalization was(17 .9 ± 6 .4)d .The survival rate had no statistical difference between the two groups (P>0 .05) .In addition ,the TMP group was superior to the C group in the average hospital days .Conclusion Eerly TMP interrention significantly reduced the rate of endo-tracheal intubation and the occurrence rate of ARDS ,shorten the hospital days ,but without increasing the survival rate .

OBJECTIVE: To establish an HPLC method for the determination of Quercetin in Duizuoye tablet. METHODS: HPLC analysis was carried out on Dikma-ODS C18 column (250 mm?4.6 mm, 5 ?m) with methanol-0.4% phosphoric acid solution(53∶47) as mobile phase at a flow rate of 1.0 mL?min-1; the detection wavelength was set at 370 nm and the column temperature was maintained at 35 ℃. RESULTS: The calibration curve for Quercetin was linear in the range of 1.31～9.17 ?g?mL-1 (r=0.999 8).The average recovery was 98.06%, with RSD of 1.05% (n=6).CONCLUSION: The adopted method is simple, rapid and accurate, and suitable for the quality control of Duizuoye tablet.

Sudden infant death syndrome(SIDS) has become a highlighted topic in forensic medicine and pediatrics since it was firstly defined in 1969.As some great improvements were preceded in many aspects,people know more about its features,summarization,classification and related genes.There are many reports from developed areas such as North America,Europe,Australia,Japan etc.,but few from China.This review introduces SIDS's development history,new scientific findings and discusses the feasibility of further SIDS studies and the key points in forensic practice under the influence of San Diego definition of SIDS.

This paper analyzes ethical issues to be concerned in the nursing process for patients' with severe degree burn,and comes up with countermeasures including building up comfortable environment of medical service,showing enough respect for patients,improving mutual communication between doctors and patients,applying flexible regulations of visiting and accompany,strengthening health propaganda,and making best efforts to maintain the original appearance and function of the burnt organs.

QT dispersion(QTd,equals maximal minus minimal QT interval on a standard ECG) has been shown to reflect inhomogeneity of myocardial repolarization and cardial electrical instability, hence,been proposed to be associated with ventricular arrhythmias.But the intrinsical mechanism of QTd is incompletely understood. Contributing to QTd are differences of action potential duration(APD) in the three-dimensional structure of ventricular myocardium, which is identified to own three cell subtypes:endocardial, midmyocardial(M cells) and epicardial cells.And findings suggest that regional differences in the duration of the M cell action potential may lead transmural and spatial dispersion of repolarization. Autonomic nerves by combining receptors on the myocardial cells,especially M cells affect significantly the APD.Concordance between heterogeneity of ventricular myocardium and heterogeneity of autonomic innervation in heart provide support for the role of autonomic nerve in the generation of the QT dispersion.

Abnormalities, Multiple ; genetics ; Acidosis, Renal Tubular ; genetics ; pathology ; therapy ; Arthrogryposis ; genetics ; pathology ; therapy ; Biopsy ; Carrier Proteins ; genetics ; Cholestasis ; genetics ; pathology ; therapy ; DNA Mutational Analysis ; Humans ; Infant, Newborn ; Mutation ; Syndrome ; Vesicular Transport Proteins ; genetics

Objective:To investigate the changes and clinical significance of body mass index (BMI), blood lipid and fat soluble vitamin levels in patients with different types of severe preeclampsia.Methods:Sixty-eight pregnant women with severe preeclampsia who received antenatal examination in Lishui Maternal and Child Health Care Hospital, China from February 2017 to February 2019 were included in the study group. According to the onset time, the pregnant women were divided into an early onset group (≤ 34 weeks of gestation, n = 38) and a late onset group (> 34 weeks of gestation, n = 30). An additional 50 healthy pregnant women who concurrently received antenatal examination in the same hospital were included in the control group. Serum levels of triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), vitamin A (VA), vitamin E (VE) and vitamin D 3 (VD 3) were determined in all pregnant women. Logistic regression was used to analyze severe preeclampsia-related influential factors. Results:There were no significant differences in age, number of births and number of pregnancies among the three groups (all P > 0.05). BMI and serum levels of VA, VE, TC, TG, and LDL-C were (22.99 ± 4.39) kg/m 2, (0.48 ± 0.08) ng/mL, (11.91 ± 1.74) ng/mL, (4.93 ± 0.34) mmol/L, (1.57 ± 0.26) mmol/L, (2.68 ± 0.27) mmol/L, respectively, which were significantly higher than those in the control group [(20.68 ± 3.68) kg/m 2, (0.40 ± 0.07) ng/mL, (10.32 ± 2.56) ng/mL, (4.12 ± 0.67) mmol/L, (1.25 ± 0.32) mmol/L, (2.15 ± 0.32) mmol/L, t = 3.725, 6.698, 4.352, 8.731, 6.282, 10.512, all P < 0.05]. Serum levels of HDL-C and VD 3 in the study group were (1.51± 0.32) mmol/L and (16.16 ± 2.37) ng/mL, respectively, which were significantly lower than those in the control group [(1.88 ± 0.57) mmol/L, (17.86 ± 2.39) ng/mL, t = - 6.959, - 4.520, both P < 0.05]. BMI and serum levels of TC, TG, LDL-C, VA and VE in the early onset group were (23.13 ± 4.13) kg/m 2, (5.05 ± 0.34) mmol/L, (1.62 ± 0.27) mmol/L, (2.95 ± 0.32) mmol/L, (0.52 ± 0.06) ng/mL, (12.16 ± 1.80) ng/mL, respectively, which were significantly higher than those in the control group ( t = 6.507, 17.462, 11.217, 16.593, 9.075 and 4.142, all P < 0.05). Serum levels of HDL-C and VD 3 in the early onset group were (1.43 ± 0.28) mmol/L and (15.76 ± 2.42) ng/mL, respectively, which were significantly lower than those in the control group ( t = 14.635, 5.871, both P < 0.05). BMI and serum levels of TC, TG and VE in the late onset group were (22.70 ± 4.32) kg/m 2, (4.67±0.32) mmol/L, (1.49 ± 0.25) mmol/L and (11.45 ± 1.61) ng/mL, respectively, which were significantly higher than those in the control group ( t = 5.821, 12.857, 8.059, 3.482, all P < 0.05). Serum level of VD3 in the late onset group was (16.72 ± 2.31) ng/mL, which was significantly lower than that in the control group ( t = 4.319, P = 0.01). There were no significant differences in serum levels of LDL-C, HDL-C and VA between late onset group and control group (all P > 0.05). There were no significant differences in BMI, and serum levels of TC, TG, HDL-C, LDL-C, VA, VD 3 and VE between early onset and late onset groups (all P > 0.05). Logistic regression analysis results revealed that BMI and serum levels of TG, TC, HDL-C, LDL-C, VA, VD 3 and VE are independent influential actors of early onset severe preeclampsia ( P = 0.000, 0.008, 0.032, 0.043, 0.032, 0.002, 0.041, 0.009). BMI and serum levels of TG, TC, VD 3 and VE are independent influential actors of late onset severe preeclampsia ( P = 0.002, 0.016, 0.013, 0.031, 0.042). BMI, blood lipid and fat soluble vitamin in combination are of high value for the prediction of early onset severe preeclampsia (sensitivity 87.81% and specificity 76.67%), but they are not of high value for the prediction of late onset severe preeclampsia (sensitivity 52.51% and specificity 55.10%). Conclusion:BMI, serum lipid and fat soluble vitamin in combination are of high value in the prediction of early-onset severe preeclampsia.

Objective To investigate effects of Livin antisense oligodeoxynucleotides(ASODN) on the proliferation and apoptosis of human leukemia(HL60) cells.Methods Livin protein on HL60 cells was examined by immunohistochemistry.Specific phosphorothioate antisense oligodeoxynucleotides and missense oligodeoxynucleotides target Livin mRNA were synthesized and transfected into HL60 cells following cationic liposome.The proliferation inhibition of HL60 cells was assessed by MTT.The expression of Livin mRNA was detected by RT-PCR.Transmission electron microscope and TUNEL technology were used to detect the apoptosis and morphologic change.ResultsASODN of 600 nmol/L inhibited the HL60 cell proliferation and the expressions of Livin mRNA.The percentage of apoptosis detected by TUNEL was 38.48%?4.37%.cellar ultrastructure was markedly destroyed by Livin ASODN.A significant difference was found when compared with the control group(P

: Insomnia is one of the most often complaints in the stroke patients and its incidence rate in the stroke patients is more higher than in general population.Insomnia can adversely affect the stroke patients' rehabilitation.Sedative hypnotics are still the most often used for insomnia after stroke currently.Furthermore,several studies have compared different pharmachotherapies and nonpharmachological treatments for insomnia after stroke.