Objective:To understand the epidemic situation of drinking-water-borne endemic fluorosis in Fujian Province, and to provide more information for control and evaluation of the disease.Methods:In 2019, all villages in the 36 drinking-water-borne endemic fluorosis areas in Fujian Province were selected, in which the situation of water improvement was investigated, fluorine content of drinking water was tested and dental fluorosis of all the children aged 8 to 12 was examined. The urinary fluoride content and skeletal fluorosis of people over 25-year old was investigated in some of those villages.Results:A total of 153 disease affected villages were investigated, in which the water improvement projects were all completed. The fluoride content in each tap water sample of the water improvement project ranged from 0.00 to 1.05 mg/L, and the qualified rate was 100.00% (153/153). The normal operation rate of the water improvement projects was 95.42% (146/153). The detection rate of dental fluorosis in children aged 8 to 12 was 2.72% (76/2 789) with a dental fluorosis index 0.07. The detection rate of children's dental fluorosis was statistically different in different age groups, so was it in areas with different coverage rate of water improvement projects ( P < 0.05). The geometric mean of urinary fluorine level in adults was 0.80 mg/L ( n = 3 765), and the detection rate of skeletal fluorosis was 2.00% (6/300). Conclusion:Although some achievements have been made in the prevention and control of drinking-water-borne endemic fluorosis in Fujian Province, it is still necessary to further consolidate and enhance the water improvement projects.

Objective To explore the relationship of urinary iodine level between 8-10 year-old children and adults under the same living conditions in coastal and mountain areas in Fujian Province.Methods Representative coastal and mountainous rural areas (Xindian Town Xiang'an District Xiamen City and Yongfu Town Zhangping District Longyan City) were selected as survey sites.Morning fasting urine samples from local 8-10 year-old children and their parents and a random urine sample in the morning from these children were collected.Urinary iodine was determined using the arsenic cerium catalytic spectrophotometric method (WS/T 107-2006).Results The median urinary iodine of fasting urine samples which were collected from 147 8-10 year-old children in coastal rural areas (71 boys and 76 girls) was 163.5 μg/L (boys 161.0 μg/L,girls 170.9 μg/L),and the median urinary iodine of random urine samples was 190.8 μg/L (boys 208.7 μg/L,girls 177.0 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.376,-0.678,all P ＞ 0.05).The median urinary iodine of fasting urine samples which were collected from 116 8-10 year-old children in mountain rural areas (66 boys and 50 girls) was 171.8 μg/L (boys 169.5 μg/L,girls 173.8 μg/L),and the median urinary iodine of random urine samples was 210.8 μg/L (boys 233.2 μg/L,girls 203.4 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.413,-0.881,all P ＞ 0.05).The median urinary iodine of random urine samples which were collected from 8-10 year-old children in mountain rural areas was significantly higher than that of the morning fasting urine samples (Z =-2.789,P ＜ 0.05).The median urinary iodine of children's fathers in coastal rural areas was 177.0 μg/L,and the median urinary iodine of their mothers was 181.7 μg/L.There was no significant difference between coastal rural children and their parents (Z =-0.387,-0.658,all P ＞ 0.05),and there was also no significant difference between fathers and mothers (Z =-0.014,P ＞ 0.05).The median urinary iodine of children's fathers in mountain rural areas was 227.3 μg/L,and the median urinary iodine of their mothers was 237.4 μg/L There was a significant difference between mountain rural children and their parents (Z =-4.176,-4.373,all P ＜ 0.05),and there was no significant difference between fathers and mothers (Z =-0.291,P ＞ 0.05).Conclusions Urinary iodine level of school-age children does not reflect the actual urinary iodine level of adults.There is a difference between urinary iodine level of random urine sample and morning fasting urine sample.Attention should be paid to this difference when monitoring iodine nutrition in population.

Objective To evaluate the influence of pregnancy outcomes with different dosage of estradiol valerate in addition to progesterone for luteal supplementation in IVF-ET cycles. Methods From February 2011 to October 2012, 362 patients undergoing IVF-ET in the second affiliated hospital of Kunming Medical University were selected. The patients were randomly assigned into three groups. Since oocyte retrieval, all of them were injected luteal supplementation with progesterone in oil 60 mg/d, im. Group A as control group (112 cases) did not add with pentanoic acid estradiol;Group B for the experimental group (122 cases), added pentanoic acid estradiol 4 mg/d after embryo transfer;Group C for the experimental group (128 cases) added pentanoic acid estradiol 6 mg/d after embryo transfer. Finally the pregnancy outcome and implantation rate were compared for each group. Results There were no significant differences between group A and group B, group B and group C in the rate of embryos grow and pregnancy rate ( >0.05) . The embryo planting rate and pregnancy rate have significant difference between Group A and group C ( < 0.05) . Conclusion 6 mg/d fill estradiol valerate for luteal supplementation may improve implantation rate and pregnancy rate after IVF.

OBJECTIVE:To provide reference for playing pharmaceutical support role of clinical pharmacist to offer medical assistance in the emergence such as earthquake.METHODS:Basing on the experience in Wenchuan Earthquake,the character and forms of earthquake medical assistance were introduced,then the role of clinical pharmacist in earthquake medical assistance were analyzed.RESULTS:Clinical pharmacists distributed effectively to the drug supply so as to save medical resources and ensure rational administration during the medical rescue in earthquake.CONCLUSIONS:For achieving better function of the treatment team,national medical rescue team should involve clinical pharmacist.

Objective Using Beckman assay kit to establish women thyroid function indicators reference interval during pregnancy in Fujian Province.Methods Two iodine sufficient areas were selected in Fujian randomly,and the women who did pregnancy test in local hospital were investigated.A questionnaire study and physical examination were conducted and women's serum samples were collected to detect the thyroid function [thyroid stimulating hormone (TSH),free triiodothyronine (FT3),free thyroxine (FT4),total triiodothyronine (TT3),and total thyroxine (TT4)] using Beckman chemiluminescence analyzer access-2 and assay kit.Reference interval was shown with median (M) and P2.5-P97.5.At the same time,the newly established reference interval was used to evaluate the thyroid function of 830 pregnant women.Result A total of 455 pregnant women were detected (early,middle and late pregnancies were 146,157 and 152,respectively).Most of the thyroid function indexes presented skewed distribution.The thyroid function index reference intervals in early,middle and late pregnancies were TSH:0.80 (0.02-2.96),1.13 (0.07-3.56),1.18 (0.09-3.76) mU/L;FT3:4.28 (3.30-5.76),3.74 (2.78-4.83),3.63 (2.81-4.56) pmol/L;FT4:11.78 (8.66-19.68),9.34 (7.03-12.76),8.55 (6.20-11.73) pmol/L;TT3:1.91 (1.09-2.95),2.00 (0.90-2.96),1.98 (0.91-3.02) nmol/L;and T4:123.00 (79.62-194.85),115.42 (58.78-175.85),117.43 (58.29-192.00) nmol/L.The thyroid dysfunction rate diag,nosed by our reference interval was 22.0％ (183/830),whilch was lower than 28.0％ (232/830) diagnosed by American Thyroid Association (ATA) reference interval (x2 =7.714,P ＜ 0.05).Conclusions Women thyroid function indicators reference interval during pregnancy in Fujian Province is successfully established.The study shows that each area and laboratory should establish the thyroid hormone reference interval of pregnant women for diagnosis and treatment of thyroid disease.

Objective To investigate the relationship between cysteine-rich protein 61 ( Cyr61 ) and phosphatidylinositol 3-kinase ( PI3K ) signal pathway on cell proliferation and apoptotic in human ovarian carcinoma cells.Methods Recombinant human Cyr61 (rhCyr61) was pretreated with ovarian carcinoma cells.The expression of Cyr61 protein was detected by confocal spectral microscopy.Then treated the ovarian carcinoma cells with PI3K transduction inhibitors (LY294002) for 24 hours.Cell apoptosis was detected by flow cytometry (FCM).Cell viability was determined by methyl thiazolyl tetrazolium (MTT) method.The mRNA expressions of Cyr61,the protein levels of protein kinase B ( PKB),phospho-PKB and Cyr61 were assaved by real time-PCR and western blot analysis,respectively.Results The Cyr61 and phospho-PKB protein expression in two ovarian carcinoma cells (OV2008 and OVCAR-3 ) were increased in rhCyr61pretreated group.The decreasing of cell apoptosis [ ( 1.4 ±0.9)％,(2.1 ± 1.0)％ ] and increasing of cell proliferation [ ( 124.0 ± 1.8)％,( 133.0 ±2.2)％ ] was detected in the same time,compared with negative control group,there were significant difference ( P ＜ 0.05 ).After exposed to LY294002 for 24 hours,the apoptosis rate of OV2008 and OVCAR-3 in pretreated with rhCyr61 group exposed to LY294002 were (21.1 ± 1.6)％ and (26.4 ± 1.5 )％,respectively.Cells viability [ (59.0 ± 2.3 )％,(51.0 ± 2.0)％ ]was also significantly decreased in OV2008 and OVCAR-3 pretreated with rhCyr61 cells.Meanwhile,the mRNA expressions of Cyr61 (3.2 ± 0.8,6.2 ± 1.1 ) and the protein levels of phospho-PKB and Cyr61 were greatly decreased.Compared with negative control group,there were significant difference in OV2008 and OVCAR-3 cells (all P ＜ 0.0l ).Conclusions The activation of PI3K intracellular signaling pathways may lead to up-regulation of Cyr61 expression.Block PI3K signal pathway could significantly inhibit the expression of Cyr61,and may promote the apoptotic effects and inhibit the cell growth of ovarian carcinoma cells.

Acquired Immunodeficiency Syndrome ; prevention & control ; China ; Health Plan Implementation ; History, 20th Century ; History, 21st Century ; Humans ; Internationality ; Public Policy ; history ; Quality Control

Objective:To investigate the relationship between gene polymorphisms of disease-relevant multiple cytokines including TNF-α,IL-6,IL-10,TGF-β1,IFN-γand acute graft versus host disease(aGVHD) in allogeneic hematopoietic stem cell trans-plantation ( allo-HSCT ) . Methods:32 cases of recipients received allo-HSCT and 36 cases of normal groups in January 2014 to December 2015 were selected as objects of study. We detected genotypes on specific SNP of target genes by polymerase chain reation ( PCR) combined with gene sequencing and observed the occurrence of aGVHD in postoperative recipients. The influence of cytokine gene polymorphisms on prognosis of allo-HSCT patients was analyzed,and the potential relationship between specific SNP mutation of the disease-relevant cytokine genes and severity of aGVHD was discussed. Results:Distribution of cytokines gene polymorphism including TNF-α-308(G/A),IL-6-174(G/C),IL-10-1082(A/G),TGF-β1+915(G/C),IFN-γ(T/A) had no significant differences with incidence of severe aGVHD(P>0. 05). However,the occurrence of severe aGVHD in allo-HSCT recipients with C/T genotype was significantly higher than C/C and T/T in SNP of TGF-β1+869(P<0. 01). Conclusion:Gene polymorphism of TGF-β1+869(C/T) in allo-HSCT patients was closely related to the occurrence of severe aGVHD. The research show allo-HSCT patients with C/T genotype occurred severe aGVHD more frequently, which is an important potential risk factor to induce the incidence of severe aGVHD. Therefore,detecting gene polymorphism of TGF-β1+869 ( C/T ) in allo-HSCT recipients and developing the appropriate therapeutic regimen may be helpful to reduce the incidence of aGVHD.

Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study.A quick, efficient,sensitive and stably method of DNA extraction from soil were established by combining strongpoints of two kits(Soilmaster kit and DNA IQ~(TM)kit).In addition,the 16S rDNA gene and T-RFLP(Terminal restriction fragment length polymorphism)were used in the analysis of soil bacterial community diversity and the result show that T-RFLP is a powerful tool for bacterial community study.

Bufonis Venenum, as an important Chinese traditional natural medicine, has complex chemical composition and has been widely used in clinical treatment with significant effects. The chemical constituents in Bufonis Venenum mainly included bufadienolides, indole alkaloids, steriods, etc. With the further research on Bufonis Venenum both at home and abroad, its chemical composition and mechanism of pharmacological activities have been more and more clearly, especially in the aspects of narcotic analgesic, cardiac, and antitumor therapy. This paper reviewed the chemical composition and pharmacology advances of Bufonis Venenum in order to provide reference to clinical use.