2.Methodology of DNA staining by Azure A.
Jian-yun ZHOU ; Song HE ; Lan YU ; Yan GUO
Chinese Journal of Pathology 2006;35(9):568-569
Azure Stains
;
chemistry
;
DNA, Neoplasm
;
analysis
;
chemistry
;
Humans
;
Rosaniline Dyes
;
chemistry
;
Staining and Labeling
;
economics
;
methods
;
Stomach Neoplasms
;
genetics
;
pathology
3.Distribution characteristics of rs10975521 A/T and rs1929992 A/G polymorphism of IL-33 gene in Guangxi population
Jing GUO ; Yang XIANG ; Youfan PENG ; Yesheng WEI ; Yan LAN
Chinese Journal of Immunology 2016;32(6):849-852
Objective: To study and explore the distribution characteristics of rs10975521A/T and rs1929992A/G polymorphism of the IL-33 gene in Chinese Guangxi population. To compare the frequency distribution differences of allele and genotype of two sites among different ethnic. Methods:The polymorphism of rs10975521A/T and rs1929992A/G of IL-33 gene in 283 subjects were analyzed with the methods of Single base extension (PCR-SEB) and DNA sequencing,and the distribution frequency and the differences between groups of that were analyzed statisticaly. Results:Three genotypes of AA,AT and TT were found in rs10975521A/T with the frequency distribution of 12. 7%,53. 0% and 34. 3% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population ( P>0. 05 ) . There were significant differences of the allele frequency of rs10975521A/T in the Guangxi population compared with that in the European (P< 0. 05), han Chinese in Bejing (P< 0. 05) and Japanese people (P< 0. 01). Three genotypes of AA,AG and GG were found in rs1929992A/G with the frequency distribution of 23. 9%,53. 7% and 13. 4% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population(P> 0. 05),but the differences of genotype frequency of rs1929992A/G was statistically significant compared with that in the European,han Chinese in Bejing and Japanese people ( P< 0. 05 ) . There were significant differences of the allele frequency between Guangxi population and European (P< 0. 01),han Chinese in Bejing(P< 0. 05). Conclusion: There are different degrees of discrepancy of rs10975521A/T and rs1929992A/G polymorphism of IL-33 gene among different race and region.
4.The value of immunocytochemistry in differentiating benign and malignant serous effusion.
Ping MEI ; Xin-lan LUO ; Yan-hui LIU ; Heng-guo ZHUANG ; Wei ZHANG ; Guo-yan CHEN
Chinese Journal of Pathology 2005;34(6):368-369
Adenocarcinoma
;
diagnosis
;
pathology
;
Adult
;
Aged
;
Aged, 80 and over
;
Ascitic Fluid
;
chemistry
;
Cadherins
;
analysis
;
Calbindin 2
;
Carcinoembryonic Antigen
;
analysis
;
Diagnosis, Differential
;
Epithelial Cells
;
chemistry
;
Female
;
Humans
;
Immunohistochemistry
;
Keratin-5
;
analysis
;
Male
;
Middle Aged
;
Pericardial Effusion
;
chemistry
;
diagnosis
;
Pleural Effusion
;
chemistry
;
diagnosis
;
Pleural Effusion, Malignant
;
chemistry
;
diagnosis
;
S100 Calcium Binding Protein G
;
analysis
6.Angioimmunoblastic T cell lymphoma with Reed-Sternberg-like cells.
Yan-hui LIU ; Heng-guo ZHUANG ; Dong-lan LUO ; Xin-lan LUO ; Jie XU
Chinese Journal of Pathology 2006;35(4):249-250
Aged
;
Antigens, CD20
;
analysis
;
Gene Rearrangement
;
Humans
;
Immunoblastic Lymphadenopathy
;
genetics
;
metabolism
;
pathology
;
Immunoglobulin Heavy Chains
;
genetics
;
Immunohistochemistry
;
Lymph Nodes
;
metabolism
;
pathology
;
Lymphoma, T-Cell
;
genetics
;
metabolism
;
pathology
;
Male
;
Polymerase Chain Reaction
;
RNA, Viral
;
analysis
;
Receptors, Antigen, T-Cell, gamma-delta
;
genetics
;
Reed-Sternberg Cells
;
metabolism
;
pathology
7.Proximal epithelioid sarcoma: a case report.
Qing-zhu WEI ; Yan-hui LIU ; Heng-guo ZHUANG ; Dong-lan LUO ; Xin-lan LUO
Chinese Journal of Pathology 2006;35(10):638-639
Antigens, CD34
;
metabolism
;
Cytokines
;
metabolism
;
Diagnosis, Differential
;
Humans
;
Immunohistochemistry
;
Male
;
Middle Aged
;
Mucin-1
;
metabolism
;
Perineum
;
pathology
;
Phosphopyruvate Hydratase
;
metabolism
;
Sarcoma
;
metabolism
;
pathology
;
surgery
;
Vimentin
;
metabolism
8.Comparison of periphery capillary whole blood glucose using BREEZE~(TM)blood glucose meter and venous plasma glucose using laboratory autoanalyzer
Jun YAO ; Yan GAO ; Li-Nong JL ; Xiao-Hui GUO ; Lan CHEN ; Po-Lan WANG ;
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Objective To evaluate the precision of BREEZE~(TM) glucose monitor and correlation of glucose measurements between fingertip capillary whole blood glucose(CBG)using BREEZE~(TM) glucose monitor and venous plasma glucose(VPG)using autoanalyzer.Methods All samples of venous plasma and fingertip blood from 188 diabetes or non-diabetes patients were detected for glucose level at fasting,30,60 and 120 min postprandial.BREEZE~(TM) glucose monitor and autoanalyzer measured CBG and VPG respectively.Intra-and inter- coefficients of variations were determined using 10 BREEZE~(TM) glucose monitors with normal,slight high and high glucose levels for the three lots of strips.Results The correlation coefficients between CBG and VPG were all higher than 0.950 at fasting and different postprandial time.98.94% of all measurements were in the A zone when using error-grid analysis.The relevant differences between CBG and VPG were less than 5% at different blood glucose concentrations.The intra-and inter-coefficients of variations of blood glucose values at different blood glucose concentrations using different lots of strips were within 5%.Conclusion BREEZE~(TM) glucose monitor provides high accurate and precise glucose readings on fasting and different postprandial time points over a variety of blood glucose concentrations.
9.Preliminary investigation in critical care medicine contents and methods for standardized training residents
Qindong SHI ; Hao LI ; Lan GAO ; Qinyue GUO ; Litao GUO ; Jinqi YAN
Chinese Journal of Medical Education Research 2017;16(6):601-604
Resident standardization training is an important means of clinical physician training in our country. Critical care medicine has important status in the training process. It is the important link to ensure the quality of resident standardization training. Residents should grasp the identification and early detection of critical ill patients. Residents should also get the ability of general basic management for critical condition and the doctor-patient communication ability. In practice, we have explored the training mode of standardized training of resident doctors in critical care medicine by developing detailed training outline, a variety of teaching methods and emphasizing the cultivation of clinical work ability.
10.Class Ⅱa Bacteriocins:Biosynthesis, Structure and Activity
Yan-Ni LV ; Ping-Lan LI ; Wei ZHOU ; Guo-Rong LIU ; Xing-Hua GUO ;
Microbiology 1992;0(05):-
Class Ⅱa bacteriocins can be considered as the major subgroup of bacteriocins from lactic acid bacteria, not only because of their large number, but also because of their activities and potential applications. They have first attracted particular attention as listericidal compounds and are now believed to be the next in line if more bacteriocins are to be approved in the future. The present review attempts to provide an insight into general knowledge available for class Ⅱa bacteriocins and discuss common features and recent findings concerning these substances.