2.Effects of different microhabitas on growth and four kinds of volatile oil components of Atractylodes lancea.
Yan ZHANG ; Sakurai MIKI ; Guang YANG ; Xiu-ji TAKEDA ; Mei-lan CHEN ; Shou-dong ZHU ; Ling WANG ; Lan-ping GUO
China Journal of Chinese Materia Medica 2015;40(21):4142-4148
To investigate the suitable site environment for Atractylodes lancea, field trials in different niches was carried out, and the seedling growth, biomass and volatile oil components in different microhabitas was studied. The study found that the survival rate, plant height, volatile oil content of those which growing under the bamboo were significantly higher than those exposed in the open field. The survival rate understory was (76 ± 15.1) %, plant height understory was (77 ± 14. 8) cm and the summation of the four kinds of volatile oil content understory reached up to 4.09%; The same evaluation values of these indicators of the four faces in the open field respectively: survival rate is 30%, plant height was (77 ± 14.8) cm and the summation of the four kinds of volatile oil content was 2.24%. But, the yield of the understory (41 ± 22.3) g was significantly lower than those four faces in the open field (104.5 g) on the contrary. For the four open field towards, the yield of the east facing, which organic matter and other nutritional conditions were better than others, was significantly higher than those in the other facings. A. lancea was found to be an anti-poor and shading-like or growing in east facing slope herb through the correlation analysis of light, temperature, soil and leaf nutrients with seedling growth, biomass and volatile oil components. It also reminds us that the understory herb with high survival rate, low incidence, low management costs, and high medicinal ingredients, although it's production is not so high, but it can be improved by increased organic fertilizer. So the ecological planting patterns which can intercropping herbs with the forest was proposed.
Atractylodes
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chemistry
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growth & development
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metabolism
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Drugs, Chinese Herbal
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chemistry
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metabolism
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Molecular Structure
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Oils, Volatile
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chemistry
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metabolism
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Seedlings
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chemistry
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growth & development
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metabolism
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Soil
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chemistry
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Temperature
3.The expression of BmK AngM1 in Mut(s) and Mut(+) recombinants of Pichia pastoris.
Qinghua WANG ; Lan LIANG ; Jingjing CHEN ; Ting GONG ; Qi HOU ; Jinling YANG ; Ping ZHU
Acta Pharmaceutica Sinica 2015;50(7):910-5
BmK AngM1 is a long-chain scorpion toxin purified from the venom of Buthus martensii Karsch. It has been reported to exhibit evident analgesic effect and low toxicity, and has the potential to be a novel analgesic drug. The BmKAngM1 gene was transformed into Pichiapastoris GS115. Mut+ and Mut(s) recombinant strains were screened by phenotype and Mut+ recombinant strains were used to detect BmK AngMl gene copy number in the real-time PCR. Expression of BmK AngM1 in the Mut+ recombinant strain was compared with that of the Mut(s) recombinant strain with the same single copy of BmK AngM1 gene under the same condition. The results indicated that the transcription level of BmK AngM1 gene in the Mut(s) recombinant strain was 2.7 fold of that in the Mut recombinant strain in the real-time PCR, and the expression of BmK AngM 1 in the Mut(s) recombinant strain was 1.5 fold of that in the Mut+ recombinant strain. Therefore, Mut(s) recombinant strain showed better ability to express BmK AngM1 than Mut+ recombinant strain.
4.Studies on macroscopic and microscopic characteristics of Ophiocordyceps xuefengensis.
Hao LIU ; Lan-ping ZHEN ; Ru-cai ZHU ; Shui-han ZHANG ; Hui-yong HUANG
China Journal of Chinese Materia Medica 2015;40(14):2820-2824
The macroscopic characteristics, tissue, caterpillar body wall and powder of Ophiocordyceps xuefengensis in different batch numbers were observed and researched by the macroscopic and microscopic identification methods. The result shows that the morphology, size, abdominal annulations of caterpillar, etc. of 0. xuefengensis are the macroscopic identification characteristics, the caterpillar body surface mycelium, body wall sculpture and crochets on abdominal legs are the microscopic identification characteristics. These characters are stable and regular discriminant features, which are proved to be the identification basis of O. xuefengensis. In addition, The characters such as crochets on abdominal legs arrange in two parallel ellipse rings, the inner crochets are long strip, and the external toes are unciform, are specific.
Animals
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Hypocreales
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cytology
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Moths
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anatomy & histology
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cytology
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microbiology
5.Synchronous cervical intraepithelial neoplasia and cervical follicular non-Hodgkin lymphoma: report of a case.
Hong ZHU ; Jian-lan XIE ; Ran YU ; Ling-ping GONG ; Xiao-ge ZHOU
Chinese Journal of Pathology 2009;38(12):841-842
Adult
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Antibodies, Monoclonal, Murine-Derived
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therapeutic use
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Antigens, CD20
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metabolism
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Antineoplastic Agents
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therapeutic use
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Cervical Intraepithelial Neoplasia
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drug therapy
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metabolism
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pathology
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surgery
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virology
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Cyclin-Dependent Kinase Inhibitor p16
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metabolism
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Cyclophosphamide
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therapeutic use
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Doxorubicin
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therapeutic use
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Female
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Follow-Up Studies
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Humans
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Lymphoma, Follicular
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drug therapy
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metabolism
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pathology
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surgery
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virology
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Neoplasm Staging
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Neprilysin
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metabolism
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Papillomavirus Infections
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Prednisone
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therapeutic use
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Receptors, Complement 3d
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metabolism
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Rituximab
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Uterine Cervical Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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virology
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Vincristine
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therapeutic use
6.Neoplasms containing small neurons: report of a case.
Ji-ping QI ; Hong ZHU ; Yu-wei CONG ; Yu-lan SUN
Chinese Journal of Pathology 2007;36(10):710-711
Astrocytoma
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pathology
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Brain Neoplasms
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Ganglioneuroma
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metabolism
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pathology
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surgery
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Glial Fibrillary Acidic Protein
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metabolism
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Humans
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Intermediate Filament Proteins
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metabolism
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Male
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Nerve Tissue Proteins
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metabolism
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Nestin
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Neurons
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pathology
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Oligodendroglioma
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pathology
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Synaptophysin
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metabolism
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Vimentin
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metabolism
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Young Adult
7.Discovery of MHC restriction in antigen recognition of T cells.
Gong-tao LAN ; Li-ping ZHU ; Wei ZHANG
Acta Academiae Medicinae Sinicae 2002;24(5):539-541
T cell can only recognize specific antigenic peptide-MHC complex on antigen-presenting cell. This is MHC restriction in antigen recognition of T cell. This phenomenon was discovered by an Austrian scientist Peter. C. Doherty and a Swedish scientist Rolf. M. Zinkernagel by chance. Then, in order to explain the phenomenon, they proposed two hypotheses: dual receptor and modified self. In the during following 20 years numbers of scientists spent great amount of time in the study of the phenomenon. The process of cell-mediated immune response becomes clear, which greatly promotes the advancing of immunology and many related disciplines.
Animals
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Antigen-Presenting Cells
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immunology
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Epitopes
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immunology
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Humans
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Immunity, Cellular
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Major Histocompatibility Complex
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immunology
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Receptors, Antigen, T-Cell
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immunology
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T-Lymphocytes
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immunology
8.Effects of Warm Neelding Moxibustion and Exercise Therapy on Scapulohumeral Periarthrits
Wei-ping LUO ; Xiao-min YUAN ; Xin-lan CHEN ; Jieyan ZHU
Chinese Journal of Rehabilitation Theory and Practice 2006;12(2):154-155
ObjectiveTo observe the effect of warm needle moxibustion and exercise therapy on scapulohumeral periarthrits.Methods90 scapulohumeral periarthrits cases were randomly divided into the synthetic treatment group and exercise therapy group with 45 cases in each group. Patients of the synthetic treatment group were treated with warm needle moxibustion and exercise therapy, but that of the exercise therapy group only with exercise therapy. Some local acupoints, for example Point LI15 (Jian Yu), Piont SJ14 (Jian Liao), Piont SI9 (Jian Zhen) and so on, were selected when warm needle moxibustion performed. Eexercise therapy includes joint mobilization and initiative function training. One course was 15 days. The effect was evaluated after two courses.ResultsApparent efficiency of synthetic treatment groups was 71% and 42% for exercise therapy groups. There was a significant difference between two groups (χ2=22.815,P<0.01). There was also a obvious difference between two groups in improving range of shoulder joint movement and alleviating extent of shoulder pain (P<0.05).ConclusionWarm needle moxibustion cooperated with exercise therapy can increase cure rate and reduce recrudescence of scapulohumeral periarthrits.
9.Effects of Long-term Tai Ji Exercise on Risk Factors of Cardiovascular Diseases and Incidence of Chronic Disease
Yong-hong ZENG ; Yan-ping ZENG ; Lin LI ; Hongxiang ZHU ; Baoling LIU ; Lan GUO
Chinese Journal of Rehabilitation Theory and Practice 2012;18(12):1148-1150
Objective To explore the effects of long-term Tai Ji exercise on risk factors of cardiovascular diseases and incidence of chronic diseases. Methods The elderly involved were divided into control group (n=62) and Tai Ji group (n=63). Both of them received 2-years health education. The Tai Ji group exercised with the frequency of 30~40 minutes each time, 3 times a week, while the control group didn't change their daily behavior. They were observed 2 and 6 years later. Results 2 years later, the blood pressure, weight and waistline decreased in Tai Ji group compared with the control group (P<0.05). After 6 years followed, 1 people died and 4 people occured cardiovascular diseases in Tai Ji group, while 2 people died and 12 people occured cardiovascular diseases in the control group. The incidence of chronic diseases was lower in Tai Ji group (9.52%) than in the control group (33.87%) (P<0.01). And the blood pressure, waistline, and hipline in Tai Ji group decreased significantly compared with the control group (P<0.001). Conclusion Long-term Tai Ji Exercise can ameliorate the risk factors of cardiovascular diseases and reduce the incidence of chronic diseases.
10.Research on climatic factors of ecology suitability regionalization of atractylodis.
Zhe-tian TAN ; Hao WANG ; Shou-dong ZHU ; Yu-ping YAN ; Lan-ping GUO ; Yu-guang ZHENG
China Journal of Chinese Materia Medica 2015;40(21):4171-4176
Through study on the correlation between atractylodis lactones ingredient content and climatic factors, we research regionalization from climatic of five main producing provinces of the country, in order to provide a scientific basis for atractylodis' conscious cultivation. By sampling from 40 origins which from five main producing provinces of the country, we use SPSS to analysis variation of atractylodis lactones ingredient content in different conditions of climatic factors and the effect of each factors. Then according to the relationship between atractylodis lactones ingredient content and climatic factors, we use ArcGIS to conduct ecological suitability regionalization based on climatic factors. The most suitable climatic condition for cultivation of atractylodis: the wettest month precipitation 220-230 mm, the warmest average temperature 25 degrees C, the average temperature of driest season 10 degrees C.
Atractylodes
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chemistry
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growth & development
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China
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Climate
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Ecology
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Ecosystem
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Seasons
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Temperature