OBJECTIVE:To investigate effect of valsanan on heart function in patients with chronic heart failure(CHF).METHODS:A total of 113 CHF patients were assigned into 2 groups.Both group received routine therapy and observation group were additionally treated with valsanan.Clinical efficacies of 2 groups before and after treatment were observed in terms of cardiac function classification,LVIDD,SV,CO,LVEF and 6 min walking test.RESULTS:In observation group,the incidence of improvement of the cardiac function was higher than in control group(P

Objective To explore the influence of vacuum sealing drainage(VSD) technology in snake bite treatment.Methods 108 patients of snake bites need to incision decompression were randomly divided into two groups,VSD group (VSD) 58 cases,conventional group (wound change dressings ordinarily) 50 cases.The secondary suture of time,the number of change dressings,material consumption fees,antibiotics use cost,wound infection rate,in hospital,average length of hospital stay were compared between two groups.Results Compared the VSD group with the conventional group,the secondary suture of time (t =11.743,P ＜ 0.05),the number of change dressings (t =50.661,P ＜ 0.05),material consumption fees (t =9.054,P ＜ 0.05),antibiotics use cost (t =21.515,P ＜ 0.05),wound infection rate (x2 =9.155,P ＜ 0.05),in hospital,average length of hospital stay (t =10.350,P ＜ 0.05) had significant differences.Conclusion VSD technique can be a ideal way to treat snake bites patients and worth popularizing at this stage.

Due to the complicated and changeable clinical symptoms,and inexact pathogenesis,leukemia was facing huge challenges in diagnosis and therapy.Aptamer could be selected through CELL-SELEX.The aptamer not only display high affinity and specificity to target cell,but also it could discriminate the specific stage of cell,such as different classification,normal and tumor cells.So CELL-SELEX has great potention in the researches of leukemia.This paper systematically summarized the advantages of CELL-SELEX,the application in specific leukemia diagnosis,targeted therapy and the discovery of cancer biomarkers.(Chin J Lab Med,2013,36:377-379)

Objective To investigate the effect of emulsified isoflurane preconditioning on focal cerebral ischemia-reperfusion (I/R) injury in rats and the mechanism. Methods Fifty-six healthy male adult SD rats weighing 250-280 g were randomly divided into 4 groups (n = 14): group Ⅰ sham operation (group S); group Ⅱfocal cerebral I/R; group Ⅲ emulsified isoflurane preconditioning and group Ⅳ fat emulsion preconditioning. Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) using a nylon thread with rounded tip inserted into internal carotid artery and advanced cranially until resistance was met. MCAO was maintained for 2 h followed by 24 h reperfusion. In group Ⅲ and Ⅳ 8% emulsified isoflurane 7.5 ml/kg and 3% fat emulsion 7.5 ml/kg were injected intraperitoneally at 24 h before MCAO respectively. Neurologic outcome was evaluated at 24 h of reperfusion and scored (0 = no deficit, 4 = unable to crawl, loss of consciousness). The animals were then killed and brains removed. The infarct size was assessed. The apoptotic cells were detected by TUNEL and calculated. The expression of Bax, Bcl-2, cytochrome C and caspase-3 protein was detected by immunohistochemical staining. Results The neurologic deficit scores were significantly lower in emulsified isoflurane preconditioning group than in I/R group. Preconditioning with emulsified isoflurane significantly decreased the infarct size and the number of apoptotic cells and increased the expression of Bcl-2 protein and inhibited the expression of Bax, cytochrome C and caspace-3 protein. Conclusion Emulsified iscflurane preconditioning can protect the brain from focal cerebral I/R by increasing Bcl-2 protein expression and decreasing Bax protein expression, and reducing cytochrome C release from mitochondria, caspase-3 activation and neuronal apoptosis.

Objective To observe the efficacy of Lipo PGE combined with Shuxuetong injection in treatment of diabetic peripheral neuropathy (DPN). Methods The 68 DPN patients were randomly divided into treatment group with Lipo PGE plus Shuxuetong injection and comparison group with Shuxuetong alone. The treatment period was 14 days. Results The pain, numbness,paresthesia and other symptoms in the limbs of patients were significantly reduced in treatment group than in comparison group (P＜0. 01). At the same time, the conduction velocity of median nerve and sural nerve was significantly increased in treatment group after treatment (P＜0.05), there were no significant differences in comparison group before and after the treatment. Conclusions The treatment of DPN with Lipo PGE combined with Shuxuetong injection is safe and effective.

OBJECTIVE To investigate the drug resistance status of Klebsiella pneumoniae,Escherichia coli and(Enterobacter cloacae) for reasonable use of antibiotics. METHODS After genus identification,drug resistance profile was analyzed by K-B method. RESULTS The predominant isolates from Enterobacteriaceae were(K.penumoniae) and E.coli.The resistant rate of the three commonly encountered bacteria in Enterobacteriaceae to antibiotics ascended yearly,with increasing isolates harboring ESBLs from 16.5%(2000) to 32.4%(2004). CONCLUSIONS Rational use of antibiotics should be obey the results of laboratory antibiotics susceptibility test to control the increasing trend and forbid outbreak of resistance.

OBJECTIVE To investigate drug resistance status of common bacteria to ciprofloxacin in hospital for reasonable use of antibiotics.METHODS After genus identification,drug resistance profile was analyzed by K-B method.RESULTS The resistant rate of common bacteria to ciprofloxacin ascended yearly from 40.8%(2000) to(51.7%)(2004),which predominated with Escherichia coli,coagulase negative Staphylococcus and S.aureus,respectively.CONCLUSIONS Rational use of fluoroquinolones should be obey the results of laboratory antibiotics suceptibility test to control the increasing trend of resistance.

Psychological disorder has a high morbidity rate among patients with cardiovascular disease. Patients with cardiovascular disease associated with depression, especially post myocardial infarction and acute coronary syndromes associated with psychological disorder, have not only a poor prognosis, but also a high mortality . Therefore, comprehensive and perfect intervention is extremely important for patients with cardiovascular disease associated with psychological disorder. The intervention for such kind patients needs an integrated disease management. It needs to consolidate the treatment of body's disease to strengthen second-level prevention of heart disease, and pay attention to the intervention for psychological disorder as well.This paper aims to provide reference for reducing the negative effects of psychological illness in patients with cardiovascular disease during clinical treatment process, and make prospects about the significance of double heart medical model, through summarizing the interaction effects of psychological factors on patients with coronary heart disease, the significance ofdouble heartnursing mode and the specific nursing measures.

In recent years, there is still an unbridgeable area in the early diagnosis of thyroid carcinoma.Many scholars are committed to the research of molecular markers, and find that RNA markers associated with thyroid carcinoma are microRNAs, human telomerase reverse transcripase, trefail factor 3 and HMGA2 genes.Different genes are closely related to the occurrence, development of different types of thyroid cancers, and some genes may even affect the transfer of thyroid carcinoma.

It is the traditional virtue of Chinese to be honest, and honest-trust is the foundation of the development of market economy. The phenomenon of honest-trust lacking in the field of medical care is a comprehensive result for many factors. It's a new subject to advocate sincere service and spread honest-trust culture for the construction of medical ethics. Therefore, we should put emphasis on the medical ethics education, strengthen the consciousness of sincere service, perfect the criteria of medical ethics, and set up the credit system of medical organization and medical staff.