OBJECTIVEThe purpose of this study was to investigate the clinical significance of THY1 protein expression in epithelial ovarian cancer.

METHODSImmunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were used to detect the protein expression of THY1, Ki67 and cell apoptosis in 76 epithelial ovarian cancers by tissue microarray. The correlation between THY1 expression and patients' clinical features was analyzed.

RESULTSOf the 76 epithelial ovarian cancer samples, 64 were informative for IHC and TUNEL assays and 42 (65.6%) among them showed down-regulated/loss expression of THY1 protein. A significant positive correlation of THY1 protein expression with clinical stage and distant metastasis was observed in this ovarian cancer cohort (P < 0.05). The more advanced the tumor stage, the more frequency of loss expression of THY1 protein. In addition, the mean positive rate of Ki67 staining in tumors with down-regulated/loss expression of THY1 was 33.7% +/- 3.5%, significantly higher than that in the tumors with normal expression of THY1 (17.3% +/- 6.1%, P = 0.0027). However, no significant correlation was observed between THY1 protein expression and tumor cell apoptosis as well as patients' survival in this series (P > 0.05).

CONCLUSIONDown-regulated/loss expression of THY1 protein in epithelial ovarian cancer is significantly correlated with cancer cell proliferation and metastasis in the epithelial ovarian cancer, and it may be used as one of the new molecular biomarkers to predict the disease progression in patients.

Adult ; Aged ; Apoptosis ; Cystadenocarcinoma, Mucinous ; metabolism ; pathology ; Cystadenocarcinoma, Serous ; metabolism ; pathology ; Down-Regulation ; Female ; Follow-Up Studies ; Gene Expression Regulation, Neoplastic ; Humans ; Ki-67 Antigen ; metabolism ; Middle Aged ; Neoplasm Metastasis ; Neoplasm Staging ; Ovarian Neoplasms ; metabolism ; pathology ; Survival Rate ; Thy-1 Antigens ; metabolism

Objective To understand the status of catheter-related bloodstream infection in Respiratory Intensive Care Unit (RICU),and explore the effect of catheter record in the prevention of catheter-related bloodstream infection (CRBSI) in RICU.Methods A quasi-experimental study was used in the study.The patients with the central venous catheters in the Respiratory Intensive Care Unit of Grade Ⅲ hospital in Beijing were enrolled.The control group was given normal care,and the intervention group was given catheter record and other nursing intervention.The infection rate of CRBSI was compared between two groups after intervention.Results Ninety-four patients were completed date collection,43 patients were in intervention group and 51patients were in control group.The infection of CRBSI was five cases (9.8％) in the control group,and 0 case in the intervention group,and the difference was statistically significant (x2 =4.453,P ＜ 0.05).The thousand-day-infection rate of CRBSI was 0.00 in the intervention group,and 4.36‰ in the control group.The catheter logs which were completely recorded were 31 (72％),and the catheter logs which were not completely recorded were 12 (28％).Conclusions A serious of nursing intervention measures including setting up catheter record,aseptic operation and pipeline observation and maintenance can decrease the infection rate of CRBSI.

Objective To redesign the clinical nursing post by scientific method and formulate a post description for ICU nurses. Methods The methods of data analysis, observation and interview were used to make a questionnaire for ICU nurses′ post in one general tertiary-level hospital, opinions of 15 experts were filtered by Delphi method from two rounds of questionnaires. Results Finally, 9 types nursing posts had been confirmed, and we studied out 9 post job descriptions. The experts′authority coefficient was 0. 84 while the had a good expert′s opinion coordination coefficient with 0. 36 (P<0. 05). Conclusions Experts′ opinions on the description of ICU clinical nursing post were concentrated and had high re liabilities in this study. It is consistent with the clinical practice and can provide foundations which includes work training, post evaluation and performance evaluation for ICU staffs.

ObjectiveTo explore the current status and influencing factors of intensive care unit (ICU) nurses' knowledge-attitude-practice (KAP) about patients' early ambulation, so as to provide guidance for formulating appropriate early ambulation intervention strategies. MethodsFrom March to April of 2018, 220 ICU nurses from 4 ClassⅢ Grade A general hospitals were selected as participants in this study by convenience sampling. General Information Questionnaire and Investigation Questionnaire for KAP on Patients' Early Ambulation were used and the multivariate stepwise regression analysis was conducted to analyze the influencing factors. ResultsA total of 211 valid questionnaires were collected. The ICU nurses achieved (7.60±1.35)points from the knowledge on patients' early ambulation and (19.13±2.25) points from the attitude and (15.02±4.13) points from the practice; regression analysis indicated that working length in ICU, and whether there was any early ambulation plan were the influencing factors of the ICU nurses' knowledge on patients' early ambulation; job title, whether had related training experience and whether there was any early ambulation plan were the influencing factors of the ICU nurses' attitude on patients' early ambulation; whether specialized in ICU and whether there was any early ambulation plan were the influencing factors of the ICU nurses' practice on patients' early ambulation(P＜ 0.05). ConclusionsICU nurses' knowledge and attitude on patients' early ambulation are good, however the practice is not satisfying. ICU managers should face up to the current status of patients' early ambulation, work out patients' early ambulation plans for this medical institution, strengthen the training for the ICU medical staff, and promote ICU patients' standard early ambulation.

Objective To evaluate the efficacy of ultrasound-guided aspiration with PICC catheter for the patients with malignant tumors.Methods Thirty patients with tumor from January to November 2012 in the General Department who were not able to draw blood directly were phlebotomized under the guidance of Site-Rite 5TM ultrasound,and the effect was analyzed.Results All 30 cases were successfully phlebotomized,with the rate of 100％.Conclusions Ultrasound-guided PICC catheter is a practical,safe and successful method to draw blood,which can improve the success rate and reduce patients' pain due to repeated punctures as well as the incidence of complications.It is a remedy for the traditional venous hemospasia and quite suitable for patients who cannot be phlebotomized successfully during the rescue.

OBJECTIVETo examine the urinary level of tissue factor (uTF) and its procoagulant activity (PCA) in patients with diabetes mellitus, and explore the relationship between uTF and renal damage in diabetes mellitus.

METHODSEighty-six patients with type 2 diabetes mellitus were divided into 3 groups according to urine albumin excretion (UACR), namely normal albuminuria group, microalbuminuria group and macroalbuminuria group. The levels of uTF, PCA, blood urea nitrogen (BUN), serum creatinine (CRE), serum cystatin C (CYSC), glycohemoglobin A1c (HbA1c), and high-sensitivity C-reactive protein (hs-CRP) were measured in all the patients and 21 healthy controls.

RESULTSCompared with normal control, the diabetic patients showed significantly increased levels of uTF and PCA. The urinary TF-PCA was positively correlated to BUN, CYSC, CRE, UACR, fasting glucose and hs-CRP, but not to uTF; only hs-CRP, UACR were positively correlated to uTF.

CONCLUSIONuTF is probably implicated in the development and progression of diabetic nephropathy.

Adult ; Albuminuria ; urine ; Blood Coagulation ; Case-Control Studies ; Creatinine ; urine ; Diabetes Mellitus, Type 2 ; physiopathology ; urine ; Female ; Humans ; Male ; Middle Aged ; Thromboplastin ; urine

Adolescent ; Adult ; Aged ; Body Temperature ; Craniocerebral Trauma ; physiopathology ; therapy ; Female ; Humans ; Hypothermia, Induced ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

BACKGROUNDDiabetes has been associated with increased risk of fracture and impaired fracture healing. The aim of this study was to examine the influence of diabetes on perioperative complications, length of stay and ambulatory ability recovery in individuals with hip fracture, and to determine whether changes could be made to improve treatment outcome.

METHODSThe study included 707 hip fracture patients treated at Beijing Jishuitan Hospital between July 2009 and December 2010. The medical history and perioperative complications were compared between non-diabetic and diabetic groups. Length of stay, days awaiting surgery, and days of hospitalization after surgery were also analyzed. Ambulatory ability was compared at 1-year follow-up using the Chi-square test and Fisher's exact test. An independent Student's t-test was used to compare normally distributed continuous data.

RESULTSPatients with diabetes were more likely than non-diabetic patients to develop cardiac perioperative complications (8.9% vs. 3.0%, P = 0.021), urinary tract infections (12.0% vs. 2.8%, P < 0.001), and gastrointestinal symptoms (15.0% vs. 6.8%, P = 0.003). No difference in perioperative complications was observed between the groups. Days awaiting surgery and length of hospital stay were both longer in the diabetic group ((8.0 ± 5.1) vs. (6.2 ± 3.7) days and (16.5 ± 3.8) vs. (13.3 ± 3.8) days, P < 0.001, respectively). Before the occurrence of fracture, patients with diabetes were less likely to be ambulatory outdoors (71.9% vs. 85.9%, P < 0.001) and had more restricted walking ability. After at least 1-year follow-up, similar proportions of patients in the non-diabetic and diabetic groups (16.1% and 15.9%, respectively), who were able to ambulate outdoors before the fracture, became housebound till the final follow-up.

CONCLUSIONSDiabetics are at increased risk of specific complications and have a longer time to surgery and longer in-hospital stay, but generally have similar recovery to non-diabetics thereafter.

Aged ; Aged, 80 and over ; Chi-Square Distribution ; Diabetes Mellitus ; physiopathology ; Female ; Hip Fractures ; physiopathology ; Humans ; Male ; Middle Aged ; Perioperative Period ; Treatment Outcome

Wulingsan， a classic prescription first recorded in the Treatise on Cold Damage （Shang Han Lun） by ZHANG Zhong-jing for patients with water retention syndrome due to the disturbance of Qi transformation in bladder， has often been modified by ancient and modern doctors for the treatment of renal diseases. It produced satisfactory outcomes without inducing adverse reactions. The databases including China National Knowledge Infrastructure （CNKI）， Wanfang Data Knowledge Service Platform， and PubMed were searched for articles concerning the clinical application and pharmacological mechanism of Wulingsan in the treatment of renal diseases published by domestic and foreign scholars in recent years. The summary of the included articles revealed that Wulingsan has been widely employed for the treatment of renal edema， diabetic nephropathy， acute and chronic glomerulonephritis， nephrotic syndrome， renal insufficiency， abnormal urination， etc.， and the resulting outcomes were satisfactory. Wulingsan alone or in combination with western medicine yielded better clinical outcomes than the western medicine alone. In the exploration of its pharmacological mechanism， there existed some individual reports by Korean scholars， while Chinese scholars tended to work as a team. After years of research， each team has found that Wulingsan was superior to Wulingsan decoction in excreting water， which might be related to the absence of massive low-polarity and volatile components in the decoction that were responsible for regulating the expression of aquaporins （AQP） in kidney of saline-loaded rats. Wulingsan regulates the endocrine state of rats with kidney yang deficiency， inhibits the reabsorption of water， increases the activity of Na⁺-K⁺-ATPase， and reduces the expression of AQP 1， AQP 2， AQP 3， and AQP 4. Besides， it bi-directionally regulates the human water metabolism， which is achieved via the dual-directional regulation of purinergic P2X3 receptor expression on bladder detrusor. The efficacy of Wulingsan in treating chronic renal failure is attributed to its protection against the damaged renal tissue，structure and fanction. Hence， this paper summarized the research progress of Wulingsan in the treatment of renal diseases in recent years， aiming to provide a reference for the clinical and basic exploration of Wulingsan against renal diseases in the future.