Treated 36 cases of infantile enuresis by acupuncturing Zuyunganqu (Foot Motor Sensory Area),Guanyuan (CV 4), Qihai (CV 6), Zhongwan (CV 12),Zusanli (ST 36), Yinlingquan (SP 9), Pishu (BL 20),Weishu (BL 21) and Shenshu (BL 23). After two courses,29 cases were cured, 5 cases were improvement and 2cases were no effect.

OBJECTIVEThis study aimed to investigate the duration of gingival healing after the stage II surgery of dental implantation for periodontitis patients and to provide clinical guidelines for implant restoration.

METHODSTwenty-nine periodontitis patients who had implantation surgery and achieved osseointegration were operated with stage II surgery (a total of 60 pieces of implants). The height of buccal gingival of each implant was measured twice after the stage II surgery. All implants were measured at the lowest point ofbuccal gingival after one week. The implants were randomly divided into four groups according to the schedule of the next test time: group one at one week from the initial test point, group two at two weeks, group three at three weeks, and group four at four weeks. Each group includes 15 pieces of implants. The amount of the buccal gingival change in each group between the second and first tests was determined, and the data were analyzed statistically.

RESULTSThe amount of gingival change of groups one, two, three, and four was (-0.25 +/- 0.66), (-0.04 +/- 0.52), (-0.70 +/- 0.77), and (-0.74 +/- 1.09) mm, respectively. No significant difference was observed between groups one and two in terms of the amount of gingival changes (P > 0.05). However, a significant difference was found between groups two and three (P < 0.05), and the amount of gingival recession was 0.66 mm. No significant difference was found between groups three and four (P > 0.05), and the gingival achieved stability.

CONCLUSIONThe gingival recession achieves stability at the fourth week (after 28 d) after stage II surgery. At this time, the implant can be restored, and the abutment can be selected according to the amount of gingival change of the periodontitis patient.

Alveolar Bone Loss ; Dental Implantation ; Dental Implantation, Endosseous ; Dental Implants ; Dental Restoration Failure ; Gingiva ; Gingival Recession ; Humans ; Osseointegration ; Periodontitis

Objective To eraluate the methods and results of endoscopic mucosal resections for colonic laterally spreading tumors with early malignant degeneration. Methods The pit pattern was studied with magnitying colonoscopy and mucosal staining technique for colonic laterally spreading tumors (LST) undergoing early earcinatous degeneration. They were removed with endoscopic mucosal resection techniques. A follow-up study was made. Results Eight patients suffering from early carcinoma on top of colonic laterally spreading tumors were followed-up. ① 75% of the lesions were situated in the rectum, sigmoid colon and decending colon. ② 75% of the lesions were larger than 30mm in diameter. ③ 75% of the lesions belonged to Ⅳpit pattern. ④ 62.5% of the lesions were Villous adenomas and 75% of the onalignant change involved the mucosa only. ⑤ All the lesions were completely removed by EMR, ⑥ No local residual lesion, recurrence or metastasis was discovered in all the patients after a mean follow-up period of 20.7 months. Conclusions Most of the early carcinomas originated from colonic LST involved the mucosa only. Endoscopic mucosal resection may be a curative method for the early cancer arising from colonic LST.

Child, Preschool ; Diagnostic Errors ; Female ; Humans ; Lung Diseases, Parasitic ; diagnosis ; Pericardial Effusion ; diagnosis ; parasitology ; Pleural Effusion ; diagnosis ; parasitology ; Trematode Infections ; diagnosis

Objective To investigate the effects of using a catheter balloon to treat crieopharyngeal achala-sia in patients with brainstem lesions. Methods Thirty cases of dysphagia caused by brainstem lesions were diag-nosed as crieopharyngeal achalasia through videofluoroscopy of swallowing.The cases were divided into a treatment group and a control group randomly.The treatment group was treated with balloon dilatation and routine dysphagia re-habilitation training once daily,while the control group was treated with routine dysphagia rehabilitation training only.The treatment end point was either the patient resuming an oral diet or after 4 weeks of treatment.All eases were evaluated videofluoroscopically with a drinking test pre-and pest-treatment.Results After 10 to 24 balloon dilata-tions,10 of the 15 patients in the treatment group regained the ability to take solid food and water orally,thoush 2 of them could take pasty food only.Only 2 of the 15 patients in the control group regained the ability to take common food by mouth,though 5 of them could take pasty food.The other patients had no improvement.There wag a signifi-cant difference between the two groups.The cricopharyngeal aehalasia of 12 patients in the ffeatment group improved from incomplete relaxation/opening to complete relaxation/opening.Pooling and residue in the pyriform sinus or val-leculae was reduced and no misaspiration was observed.In the control group only 7 patients had some improvement.The mesn time for the bolus passing the pharynx after treatment was significantly shortened from 0.23 s to 0.15 s in the treatment group,but not significantly in the control group.Conclusions Catheter balloon dilatation is effective for cricopharyngeal achalasia caused by brainstem injury and is helpful for relieving the symptoms in the pharynx phase and the esophagus phase of dysphagia.

Objective To compare 76% meglucamine diatrizoate solution with 60%barium sulphate suspen-sion for use in videofluoroscopic swallowing studies(VFSSs).Methods Forty-nine cases of dysphagia caused by brain injury.brainstem lesion ir nasopharyngeal carcinoma(NPC)were recruited for this study.They were divided into a meglucamine diatrizoate group of 22 patients who were administered 76% meglucamine diatrizoate solution as a contrast agent,and a barium sulphate group of 27 patients with whom 70%barium sulphate suspension was used.All the Datients were treated by balloon dilatation and other routine dysphagia rehabilitation procedures.The treatment end point was either the patient's resuming an oral diet or after 4 weeks of treatment. All cases were evaluated by VFSS pre-and post-treatment.Results The patients in the meglucamine diatrizoate group showed significant pre-and post-treatment differences in terms of the pharynx transit times of brain injury and brainstem lesion victims.NPC patients showed no significant differences.In the barium sulphate group there were significant pre-and post-treatment differences in Dharynx transit time for patients with all three conditions. Conclusions Using 76%meglucamine di-atrizoate solution as a contrast agent decreases the sensitivity of VFSS. Using 60% barium sulphate suspension in VFSS is recommended.

Objective To investigate any correlation between walking capacity, center of mass, and pelvic and hemiparetic lower limb motions in stroke survivors. Methods The kinematic variables studied included maximum walking speed, center of mass ( CoM ), pelvic movements and lower-limb movements on the paretic side. These were examined using a three-dimension motion analysis system as thirty-two post-stroke hemiparetic patients walked without aids. Results Average walking capacity was significantly correlated with lateral shifting of the CoM, with paretic limb hip extension, and with deficient knee flexion motions. There was a significant correlation between lateral CoM shifting and paretic limb hip extension and also deficient knee movement. Conclusions Walking capacity after stroke is associated with the lateral shifting of the CoM, paretic limb hip extension and deficient knee flexion. The lateral CoM shifting was associated with paretic limb hip extension and deficient knee movements.

OBJECTIVEThis research explores the regulatory role of bone morphogenetic protein 2 (BMP2) in the expression of sclerostin in OCCM-30 cementoblast.

METHODSOCCM-30 cementoblasts were treated with 50 and 100 ng · mL⁻¹ BMP2 for 3, 5, and 7 days. SOST mRNA was detected by real-time quantitative polymerase chain reaction (RT-PCR). Western blot analysis was employed to detect the sclerostin levels in the nucleus. Five groups were prepared for the experiments: control, BMP2, BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059. OCCM-30 was pretreated with BMP2 for 3 and 5 days, and then the sclerostin and SOST mRNA levels were measured.

RESULTSRT-PCR and Western blot analyses showed that BMP2 upregulated the expression of SOST in a concentration-dependent manner. SOST expression increased with time (P < 0.05). Moreover, sclerostin levels of BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059 groups were lower than that of the BMP2 group, and the sclerostin level in BMP2+dorsomorphin group was lowest (P < 0.05).

CONCLUSIONThe upregulation of SOST by BMP2 in OCCM-30 is mainly mediated by the BMP2/Smad signal pathway.

Blotting, Western ; Bone Morphogenetic Protein 2 ; metabolism ; Bone Morphogenetic Proteins ; metabolism ; Dental Cementum ; metabolism ; Genetic Markers ; Signal Transduction ; Up-Regulation

Objective To observe the clinical effect of HuaYu TongFu KaiQiaoFang on serum homocysteine (Hcy)in patients with acute cerebral infarction. Methods Sixty cases of acute cerebral infarction admitted into the comprehensive ward and intensive care unit(ICU)of the First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine from December 2010 to January 2012 were randomly divided into two groups,each group including 30 cases. The control group was given conventional treatment,and based on the conventional therapy,the treatment group was additionally given HuaYu TongFu KaiQiaoFang(a traditional Chinese medicine decoction including the following ingredients:Safflower 10 g,Chishao 10 g,Chuanxiong 10 g,Notoginseng 10 g,Angelica Sinensis 10 g, Shichangpu 10 g,Polygalae 10 g,Glauber's salt 5 g,Rhubarb 6 g),one dose a day by oral or nasal administration for consecutive 14 days. Before and after treatment in the two groups,the serum Hcy level was detected by enzyme-linked immunosorbent assay(ELISA),and the clinical efficacy was assessed by Pearson or Spearman correlation analysis to observe the correlation between neurological deficit score(NDS)and Hcy levels. Results Before treatment, the difference of serum Hcy(μmol/L)and NDS levels between the two groups was not statistically significant(both P>0.05),while after treatment,the levels in the two groups were significantly decreased in comparison with those before treatment(P<0.05 or P<0.01),and the degree of descent in treatment group was more obvious compared with that in control group(Hcy:7.78±2.51 vs. 10.18±2.41,NDS:10.60±2.25 vs. 14.60±3.70,both P<0.05). After treatment,the total effective rate in the treatment group was significantly higher than that in the control group (90.0%vs. 76.7%,P<0.05). Before treatment,the NDS was positively correlated with Hcy(r=0.675,P<0.05). Conclusion HuaYu TongFu KaiQiaoFang can significantly reduce the level of serum Hcy and improve the nerve function,thus its clinical effect for treatment of acute cerebral infarction is remarkable.

Objective To explore the therapeutic effect and possible mechanisms of Xuebijing injection for treatment of acute pulmonary thromboembolism(APE)in rabbits. Methods Twenty-four New Zealand rabbits with big ears were randomly divided into control group,model group and Xuebijing group(each n=8). The model of APE was performed by re-infusing of autologous blood clots. After the model was established,in the rabbits of the Xuebijing group,Xuebijing injection 1 mL/kg was injected into the ear marginal vein,twice daily for consecutive 7 days. In the model and control groups,the same amount of normal saline was given via the same route. The changes in plasma levels of B-type natriuretic peptide(BNP)were detected by enzyme-linked immunosorbent assay(ELISA) at the time points of 8 hours and 1,3,5,7 days,and after 7 days the lungs of the rabbits were removed for the lung histopathological examination. Results The BNP levels of the model group and Xuebijing group were significantly higher compared with the level of control group at 8 hours after embolization,the model group being the most significant(all P<0.05). On day 3,the BNP levels of the model group and Xuebijing group reached their peaks,and then gradually decreased,but the level of model group was significantly higher than that of Xuebijing group(ng/L:581.1±144.6 vs. 251.5±22.5,P<0.05). On day 7,the BNP level of Xuebijing group fell to the level of the control group(ng/L:78.5±9.4 vs. 50.9±11.5),the level of the model group also decreased,but it was still much higher than that of the Xuebijing group(ng/L:214.1±47.6 vs. 78.5±9.4,P<0.05). Histopathological examination showed Xuebijing could significantly reduce the infarct size with mild inflammatory cell infiltration,but the infarct size of the model group was obviously bigger than that of the Xuebijing group and accompanied by relatively more inflammatory cell infiltration. Conclusion Xuebijing injection can significantly reduce the plasma BNP level of the rabbits with pulmonary embolism,and in the mean time ameliorate the degree of inflammatory infiltration in the lung tissue and infarct.