OBJECTIVE:To explore the inhibitory effects and mechanism of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge.METHODS:The experimental endometriosis in female rats was established with sponge implantation.The effects of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge were observed after intragastric medication for 2 consecutive weeks.The staining of endothelial cell specific Ⅷ factor and vascular endothelial cell growth factor(VEGF) was carried out by immunohistochemistry method.The expression of VEGF mRNA was detected by RT-PCR assay.RESULTS:In rats receiving high dosage of Rhizoma Sparganii and Rhizoma Curcumae(SC),the area of new granulation tissue in sponge,the number of blood vessels,the expression of VE-GF and VEGF mRNA were all significantly lower than in model group(P

OBJECTIVE:To provide reference for the construction of suitable evaluation index system for the implementation of National Essential Medicine System in community health service center.METHODS:The frame of evaluation index system was designed by theoretical analysis,literature study and expert interviews methods.Delphi method was used to establish evaluation index system for National Essential Medicine System in Shanghai community health service center.Analytic hierarchy process was adopted to determine the weights of indicators at all levels.RESULTS:The positive coefficients of the two rounds consultations were 88.24％ (15/17) and 86.67％ (13/15).The authority coefficient of structural index,processing index and result index in the first round consultation were 0.68,0.68 and 0.69,respectively,which showed that the authority degree was higher.The coefficient of concordance in the second round consultation was higher than the first round (the indexes of structure,process and result in two rounds consultation were 0.286,0.306,0.193 and 0.345,0.358,0.337,respectively),which indicated the expert opinions were highly consistent.Established evaluation index system consisted of 3 fiust-level indexes,13 second-level indexes and 27 third-level indexes.CONCLUSIONS:The selected experts were representative,the consultation results were reliable,established evaluation index system was rational and the setting of weights was scientific.Also this study was a methodological exploration.

OBJECTIVE:To discuss the necessity and strategies to establish credibility in drug retailing stores.METHO- DS:The general meaning of credibility and its particular meaning in medicine were explained.The significance of establishing credibility in drug stores was analyzed,and the relevant strategies were suggested.RESULTS & CONCLUSION:Now in the vigorous market competition, only through establishing credibility can drug stores survive and develop.

OJBECTIVE:To provide reference for realizing talents internationalization of pharmaceutical enterprises in China.METHODS:Applying the related theory of human resources management,the necessity and situation of Chinese pharmaceutical enterprises in talents internationalization were analyzed,and the development strategy was put forward.RE?SULTS&CONCLUSION:Against the background of world-wide competition of talents in pharmaceutical industry,only through adoption of the corresponding development strategy and active advancement of talents internationalization,will the pharmaceutical enterprises dominate in the new competition.

OBJECTIVE:To explore the therapeutic effect of Chinese herbal compounds Zhiganle on fatty liver induced by high-cholesterol high fat diet in rats and the action mechanism.METHODS:Wistar rats were randomized into 6 groups:normal group,model group,Dongbao gantai group,ZGL groups(high,middle,and low doses).Except the normal group,the other 5 groups of rats were administered with high-cholesterol high fat diet for 12 weeks.On week 13,the ZGL groups were administered intragastrically with Zhiganle(1.5,3.0,4.5 g?kg-1) and rats in Dongbao gantai group with Dongbao gantai(0.3 g?kg-1) qd for 12 consecutive weeks.The hematological indexes were measured and the pathological change in rats was observed.RESULTS:In ZGL groups,serum levels of total cholesterol(T-CH) and triglyceride(TG) and the MDA level in liver were decreased significantly(P

To set up rat myocardial infarction model and to investigate significance of fragmented QRS wave in rat myocardial infarction model.40 rats were divided into sham-operated group and myocardial infarction group, these two sets of rats received surgeries under the same conditions.Myocardial infarction models were induced by opening chest and anterior descending branch of coronary artery deligation for rats.Rats in sham-operated group were only threaded without deligation.Recorded the electrocardiogram(ECG) during 10, 60 min and 4 weeks after the deligation(or threading) and analyzed the change rules of the fragmented QRS waves and Q waves.Tissue samples from myocardial infarction area were collected for HE staining 4 weeks after the surgery.The frequency of fragmented QRS wave in myocardial infarction group was significantly increased during 10, 60 min and at 4 weeks after the operation compared with sham-operated group(P<0.01).Myocardial cells of some models in myocardial infarction group arranged in disorder and showed vacuolation according to results of HE staining.By setting up rat myocardial infarction model, we can draw a conclusion that fragmented QRS wave can be used as a new indicator to diagnose acute myocardial infarction and old myocardial infarction predict the severity of the disease.

OBJECTIVE:To provide references for the evolvement of Chinese drug safety credit system construction.METHODS:The status quo of Chinese drug safety credit system was analyzed by applying the credit management theory,and some corresponding developmental countermeasures were put forward by drawing experiences from abroad.RESULTS &CONCLUSION:The fundamental strategy for the construction of standardized economic order in pharmaceutical market is to establish and perfect drug safety credit system.The drug safety credit system should be constructed comprehensively from the credit laws,credit information databank,credit ratings,credit supervision,credit agency,credit awards and punishments,etc.

OBJECTIVE: To provide references for promoting the development of Chinese bio-pharmaceutical industry. METHODS: The development of American bio-pharmaceutical industry was analyzed, and their advanced experiences on bio-pharmaceutical industry policy were investigated and drawn. RESULTS & CONCLUSION: Bio-pharmaceutical industry policy is essential in ensuring fast development of bio-pharmaceutical industry. Based on the advanced experiences drawn from American, together with the practicality, we should formulate bio-pharmaceutical industry development policy that tailored to our own specific condition.

OBJECTIVE:To prepare Xiaocuo emulsion and to study its stability.METHODS:The formula and techniques were optimized with metronidazuo,cimetidine,chloramphenicol and salicylic acid as the chief ingredients,and with the uniformity of emulsion as the indicator.The stability test was performed using storage test and accelerated centrifugal test.RESULTS:The optimized formula was the following,5ml azone,4ml tween-80,1g metronidazole,2g cimetidine,2g chloramphenicol,1g salicylic acid and 100ml deionized water.CONCLUSION:The preparation is reasonable in formula,simple in preparative techniques,stable in quality and feasible in production.

Objective To observe leptin level before and after rosiglitazone administration and to explore the possible mechanism of rosiglitazone's improving insulin sensitivity.Methods A total of 30 patients newly diagnosed with type 2 diabetes were separated into three groups at random,and treated with rosiglitazone(4mg/d),metform(750mg/d) and gliclazide(160mg/d),respectively.Serum leptin,insulin and GHbA1c were measured after 12 weeks.Results After 12 weeks' treatment,the fasting glucose and GHbA1c in each group declined sharply,while free insulin did not change.The leptin level in rosiglitazone group decreased and insulin sensitivity was improved after administration,but there was no change in metformin and sulfonylureas groups.Conclusion Leptin may play a role in rosiglitazone's mediating the insulin-sensitizing effects.