Objective To study the optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum. Methods Taking S_3 stain of G. lucidum as test materials and laccase activity of G. lucidum as measurement index to optimize the culture media and liquid conditions through orthogonal test. Results The optimum culture components of media were: Glucose 30 g/L, cotton 0.2%, (NH_4)_2HPO_4 0.66 g/L, casein 0.5%, Tween-80 0.15 mL; the optimum conditions were: initial pH value of medium was 5.5, 75 mL medium was in 250 mL-flask, inoculation was 12.5%, mycelium age was 5?24 h for 9?24 h culture. Conclusion Laccase activity of G. lucidum has been improved remarkably in the optimum culture media and liquid conditions.

Objective Three kinds of different internal promoters were inserted into the lentiviral vector to drive the expression of CD19-specifc chimeric antigen receptor (CAR)-redirected T lymphocytes with the green fluorescent protein(GFP) and the GFP expression efficiency of transduced cells driven by lentivirus were compared.Methods Three GFP reporter lentivirus vectors carrying different promoters and anti-CD19-CAR,including CMV,EF1α,and CMV-EF1α were selected.Three different vectors' names abbreviate to pHAGE-CMV-EFlα-EGFP-CD19,pHAGE-CMV-EGFP-CD19,pHAGE-EF1α-EGFP-CD19.Human embroic kideny 293T cells were cotransfected with the three plasmids by calcium phosphate DNA precipation.And the expression of GFP was observed under fluorescent microscope after transfecting 293T cells,and virus supernatant was collected after 72 h and centrifuged.Nucleic acid copy number in RNA and the expression of EGFP and CD3 zeta were identified through fluorescence quantitative PCR,flow cytometry and western blotting,respectively.The titers of the lentiviral vectors were determinde by scoring GFP expression following swerial dilutions of the viral supernatant on 293T cells.T lymphocytes was infected with lentivims produced from these reporter vectors.Then,fluorescence microscope,wesyern blotting were used to detect the GFP expression strength.Results Results of fluorescence microscope maintained that different internal promotors driving the expressing effect is different.293T as targeted cell,the transfected 293T cells were found containing strong expression of GFP.The amount of the 293T cells expressing GFP driven by CMV-EF1α promoter was the largest,while the EF1α promoter was the smallest.Flow cytometry results show that the rate of transduction is 72.4％,20.6％ and 14.5％,respectively.T lymphocytes as targeted cell,Western blot showed that CMV-EF1α was the largest among all promoters,while the CMV was the smallest.Conclusion In the study of gene expression mediated by lenuvirus,proper cell lines and promoters should be selected to obtain high efficiency.

This study was designed to evaluate the effect of estrogen to the growth of leiomyoma and the mechanism of testosterone propionate action on leiomy-oma.The levels of estrogen receptor in uterine tissud and those of estradiol and progesterone in uterine tissue and plasma were determined in 47 women with leiomyoma.The levels of estrogen receptor and estradiol in leiomyomatous tissues were 37.6?4.0 fmol/mg protein and 401.7?92.6 pg/g tissue,respectively.Both of them were higher than the corresponding levels in normal uterine tissues (P

Preoperative radiotherapy combined with chemotherapy and (or) targeted therapy not only reduces the local recurrence of locally advanced rectal cancer and increases the rate of anal preservation and pathologic complete response,but also improves patient compliance.Preoperative neoadjuvant therapy will gradually become the standard treatment for locally advanced rectal cancer.

Objective　To explore the changes and significance of the circulating immunocomplexes(CIC) in patients with chronic iridocyclitis.Methods　We assessed circulating immunocomplexes in peripheral blood of 45 patients with recurrent uveitis and 42 healthy controls by PEG deposition with the biochemical analyzer to obtain levels of CIC different with recurrent uveitis.Results　Levels of CIC were significantly higher in active patients(27.358±6.729)than those in controls(13.459±6.164,P＜0.001) and inactive patients (16.248±6.233,P＜0.001),but patients (inactive) had significantly higher levels than those in controls (P＜0.05).Conclusion　Levels of CIC were found in the groups of active patients with recurrent uveitis (27.358±6.729), but levels of CIC in inactive patients fell. The result of this study suggest that recurrence of uveitis is related to immunocomplexes,and changes in the levels of CIC could be also a measure standard of therapeutic effects.

Objective: To investigate the depression and anxiety in patients with asthma. Methods: With SAS and SDS,100 asthmatic patients were assessed. Results: The mean scores of SAS and SDS were 54.12±6.47, 44.8±7.28 respectively. 68% of sample had anxiety and 78% had depression. Conclusion: Most of patients with asthma have anxiety and depression. It suggests the treament of depression and anxiety for asthmatic patients are needed besides the conventional treatment.

Humans ; Intention ; Personnel Turnover

OBJECTIVE:To discuss the effect of Ethinylestradiol and cyproterone acetate tablet combined with metformin on ovulation induction of patients with polycystic ovarian syndrome(PCOS). METHODS:184 PCOS patients were randomly divided into control group(92 cases)and observation group(92 cases). Control group orally received 1 Ethinylestradiol and cyproterone ac-etate tablet in 5 d of menstruation,once a day,it stopped after 21 d,the next cycle of treatment was started on the 5th of with-drawal bleeding. Observation group additionally received 3 Metformin tablet,once a day,without drug withdrawal during menstrua-tion,and lasted for 3 menstrual cycles. After 3 menstrual cycles,all patients received ovulation induction treatment [(50-100 mg clomiphene was orally given from the 5th day of the 4th menstrual cycle,for 5 days,or 75-150 U postmenopausal gonadotropin by intramuscular injection,when the dominant follicle diameter was(19.0±2.0)mm,10 000 U human chorionic gonadotropin(HCG) was intramuscularly injected,the ovulation was continuously monitored and whether there was ovarian hyperstimulation syndrome (OHSS)was observed]. Body mass index(BMI),follicle estrogen(FSH),luteinizing hormone(LH),prolactin(PRL),estradiol (E2)and testosterone(T)before and after treatment,fasting plasma glucose(FPG),fasting insulin(FINS),insulin resistance in-dex(HOMA-IR),pregnancy rate,ovulation rate and the incidences of OHSS and adverse reactions in 2 groups were observed.RE-SULTS:Before treatment,there were no significant differences in the BMI,FSH,LH,PRL,E2,T,FPG,FINS and HOMA-IR between 2 groups(P>0.05). After treatment,BMI,LH,PRL,E2,T,FPG,FINS and HOMA-IR in observation group and LH, E2 and FINS in control group were significantly lower than before,and BMI,PRL,E2,T,FPG,FINS and HOMA-IR in observa-tion group were lower than control group,the differences were statistically significant(P<0.05). The pregnancy rate and ovulation rate in observation group was significantly higher than control group,the incidence of adverse reactions was significantly lower than control group,the differences were statistically significant (P<0.05). And there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Ethinylestradiol and cyproterone acetate combined with metfor-min can effectively improve insulin resistance of POCS patients,recovery sex hormone secretion,promote ovulation and improve pregnancy rate,with good safety.

Objective: To make a comparative study on the differential diagnosis of the acute myeloid leukemia based on French-American-British Cooperative Group (FAB) and World Health Organization (WHO) standard. Methods: The 118 patients were recruited who were diagnosed with de novo AML. All cases were re-classified according to FAB and WHO classification standard. The median survival time and overall survival rate were recorded for prognosis evaluation. Results: Based on WHO classication standard, there were 35 AML patients (33.7%) with recurrent cytogenetic abnormalities, 21 patients (20.2%) with multilineage dysplasia, and 2 patients (1.9%) with therapy-related AML,and 46 patients (44.2%) AML not otherwise categorized. The 3-year survival rate were (75.1 ± 7.8)%, (47.4 ± 13.9) %, (50.0 ± 35.4)%, and (54.9 ± 10.0)% in different groups based on WHO standard. The difference was significant (P <0.001). Different karyotypes of chromosome had significant effects on the prognosis of AML. The median OS rate was (87.4 ±6.9)% for patients with good prognosis, (69.3 ±7.3)% for patients with moderate prognosis, and (19.6 ± 10.9)% for patients with poor prognosis. The difference was significant (P <0.001). Conclusion: Our study results suggest that classification of primary AML patients according to the WHO standard was more effective for evaluating progression and prognosis of AML.

Objective: To analyze the clinical efficacy of hematopoietic stem cell transplantation (HSCT) in the treatment of hematological malignancies and compare the survival rate between the patients who were at different stages of disease and received different preconditioning regimen. Methods: Of the 38 patients, 25 patients received allogeneic transplantation and 13 patients underwent autogeneic transplantation. According to the phases of disease the patients were divided into two groups, standard-risk group (26 cases) and high-risk group (12 cases). The study was terminated by the death of the patients or after last follow-up. The overall survival (OS), transplant-related mortality (TRM), and the relapse rate were analyzed. Results: The 6-year expected OS, TRM, and the relapse rate were (50.36 ± 9.36)%, (21.47 ± 8.17)%, and (35.93 ± 9.89)%, respectively. The 6-year OS was(60.10 ± 10.39)% in standard-risk group. The 9-month OS was significantly higher in standard-risk group than that in high-risk group [(78.59 ± 21.41)% vs (28.86 ± 16.58)%; P=0.0118)]. The expected 6-year relapse rate of standard-risk group was significantly lower than that in high-risk group [(26.2 ± 10.16)% vs (74.07 ± 21.67)%, P=0.0062]. There was no significant difference in the 6-year OS between the patients who received allogeneic and autogeneic HSCT [(54.52 ± 10.87)% vs (40.00 ± 17.38)%, P=0.70]. The expected 6-year OS of patients who received total body irradiation (TBI) was superior than those who did not receive TBI preconditioning [(57.10 ± 10.44)% vs (22.86 ± 18.72)%, P=0.029]. Conclusion: HSCT is an effective method for the treatment of hematological malignancies. The long survival and relapse rate are significantly influenced by the stage of disease and preconditioning regimen.