Altitude ; Animals ; Blood Glucose ; metabolism ; Cholesterol ; blood ; Hypoxia ; metabolism ; Mice ; Weight Loss

Objective To summarize our experience with combined cardiac pacing and beta-blocker therapy in patients with long-QT syndrome. Methods Nine patients with long-QT syndrome were treated with Propranolol. Its effectiveness was analyzed during matched periods before and after starting beta-blocker therapy. If sinus bradycardia, sinus arrest and atrioventricular occurred during the Propranolol treatment, DDD pacemaker were implanted. Results After initiation of beta-blockers, there was a significant reduction in the rate of cardiac events [(0.86?0.92) to (0.34?0.69) events per patient per year, P

Objective The aim of this study was to asses the success rate, lesion characteristics and complication in acute coronary syndromes treated by direct coronary stenting Methods 92 patients with acute myocardial infarction and unstable angina were divided into the direct stenting ( n =32) and stent implantation with balloon predilatation (conventional stenting, n =60) groups The clinical data, characteristics of target vessels ,success rate and complications were compared between two groups Results The age in direct stenting groups was much younger than that in conventional stenting group (53 14?9 18 vs, 64 28?12 36, P

Objective: To study the effect of phytic acid (PHA) on 1,2-dimethylhydrazine (DMH) induced colorectal carcinogenesis in rats. Methods: Thirty four weeks old healthy male Wistar rats were randomly divided into two groups,control group given basal diet and PHA group given basal diet plus 2% sodium phytate solution. Both groups were injected DMH weekly for 20 w and then killed . Body weight, mortality, tumour incidence and average size, and natural killer(NK)cell activity were measured. Result: The mortality was lower in PHA group (P

The present investigation was undertaken to evaluate the role of the Chinese fruit juice in blocking nitrosamine formation in vitro.A model system simulated the conditions (pH 3.3, 37℃) known, to exist in the stomach, was used in the experiments. Different amount of nitrite and aminopyrine was incubated with Chinese fruit juice or vitamin C solution or buffer solution for 1 hr. 0.1 ml samples were taken and then subjected to the Ames mutagenicity test. The tester strain TA100 of Salmonella typhi-murium was employed for the detection of mutagenicity due to the N-nitro-samine formed from sodium nitrite and aminopyrine. Test samples and bacteria with or without S-9 mixed with molten top agar and poured onto the minimal agar plates. After incubation, the sample, that gave at least a 2-fold increase in induced revertants, compared to spontaneous revertants, was considered to have mutagenic activity.It showed that the concentration of the precursors was at or above 5 mg/ ml with S-9 and without juice. the samples exhibited mutagenic activity. It suggested that the nitrosamine was formed in this system. While the incorporation of Chinese fruit juice was found to inhibit the formation of nitrosamine at 5mg/ml and 8mg/ml. The cultures did not show mutagenic activity. The induced revertant colonies per plate was 252 ? 4.2. The Chin esefruit juice is more effective than that of vitamin C, the induced revertant colonies per plate was 445 ? 81.2, which shows mutagenic. The difference between the cultures of Chinese fruit juice and vitamin C is significant (P

Objective To investigate the serum level of vascular endothelial growth factor (VEGF) in patients with coronary artery disease (CAD) and its clinical significance. Methods The study included 36 patients with CAD and 10 non-coronary heart disease patients as the control. The concentrations of serum VEGF in the coronary veins and the aorta were measured by an enzyme-linked immunsorbent assay (ELISA). Total mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation, and were cultured in M199 medium supplemented with 20% fetal bovine serum. After 7 days culture, attached cells were collected. The effect of VEGF on the migration of endothelial progenitor cells (EPCs) were assayed with modified Boyden chamber assay. Results The serum VEGF levels of stable angina pectoris (SAP) patients (208.46?44.74 ?g/L) and acute coronary syndrome (ACS) patients (267.29?93.99 ?g/L) were much higher than that of the controls group (120.77?26.25 ?g/L,P

Objective To evaluate the clinical feasibility of disruption of intracoronary plaques using catheter-delivered ultrasound. Methods Ultrasound was introduced via catheterization to treat patients (n=18) with serious stenosis of the coronary artery. Comparison of stenosis degree and ST segment in routine ECG before and after the treatment was conducted. Pre- and post-treatment exercise tests were performed to measure the time needed for induction of angina, the time needed for induction of decrease in ST segment for 0.1 mV and maximal range of decrease in ST segment. Results Ultrasound was found to have dissolved atherosclerotic plaques in 18 cases of coronary heart disease with stenosis in one blood vessel ≥75%. The residual stenosis was (40.11?15.33)% after the treatment, representing a decrease of 44.58% as compared with that before the treatment [(84.69?4.69)%], P

To analyze the correlation between fragmented QRS complexes( fQRS) and inflammation in 136 patients with acute coronary syndromes(ACS). Patients with fQRS had increased hs-CRP levels (P<0.01) in comparison to patients with non-fragmented QRS. The higher hs-CRP levels was,the more the number of leads in the fQRS on ECG was(P<0.01). When we performed multiple logistic regression analysis, fQRS was found to be related to in-creased hs-CRP levels [ OR:1.507 , 95% CI:1.172~1.936 , P<0.01 ] . fQRS was independently related to in-creased hs-CRP. fQRS that may result as an end effect of inflammation at cellular level can represent increased car-diac risk in patients with ACS.

Objective To explore the relationship of the plasma ox-LDL level and endothelium flow mediated vasodilation(FMD)in acute coronary syndrome patients(ACS).Methods From March to October 2004,in the Department of Cardiology,the Fourth PLA Hospital,51 patients with ACS were divided into 2 groups:AMI group(22patients)and UAP group(29 patients).20 subjects with normal coronary angiograms served as the control group.Nitrir oxide(NO)and plasma ox-LDL was measured by spectrophotometer and Sandwich ELISE method,respectively.The brachial arterial hyperemia-induced flow mediated vasodilation(FMD)and sublingual nitroglycerin(NTG)mediated vasodilation were measured by high resolution ultrasound.Results (1)The FMD in ACS group was significantly decreased compared with the control group[(7.2?1.42)、(7.6?1.12)vs(15.46?1.2),P0.05).(2)The No in ACS group was sigificantly lower than that in control group;the NO in AMI group was even lower[(48.46?12.44)、(60.42?10.30)vs(94.72?12.34)?mol/L,P1,which meant ox-LDL was a risk factor;the OR of FMD was

Objective To investigate the effect of cytochrome P_ 450 2A6 (CYP2A6) genetic polymorphisms on serum concentration of sodium valproate. Methods A total of 98 epileptic patients receiving sodium valproate after a period of more than 5 half-time were recruited. The genotypes of CYP2A6 of the patients were detected by nested-primer polymerase chain reaction (PCR) to examine the alleles CYP2A6*1 and CYP2A6*4. Fluorescence polarization immunoassay (FPIA) was used to measure the serum concentration of sodium valproate. Results Of the 98 cases, 73 (74.5%) were wild genotypes, 24 (24.5%) were CYP2A6*1/*4 genotypes and 1(1.0%) was CYP2A6*4/*4 genotype. According to the genotypes of CYP2A6 the patients were divided into two groups,one was group A (CYP2A6*1/*1) and the other was group B (CYP2A6*1/*4 or *4/*4). The mean value of the serum concentration of sodium valproate of the patients in group A(4.1393?0.2793) was higher than that in group B(3.3486?0.3919) with a statistical significance (P