The oral community survey in Yen Bai was conducted in 1997. 804 pupils (girls and boys aged 6 to 11 years old) who were ethnic minority primary pupils were examined for Dental caries and periodontal status and some of concerned elements using standard 1988 WHO basis oral health. Survey method and test question. Percentage of pupil affected by Dental carries of milk teeth: 41.79%, highest in Kinh group, lowest in Tay group. It is not alien from delta area and highland. Periodontal status: percentage of pupils who had CPI1=4.48%; CPI2=47.39%. It is not alien from delta area and highland but it is alien among nations, highest CPI2 was in H’Mong group. It needs a project to prevent reasonably oral diseases.
Tooth Diseases ; Pupil

Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.