Objective To investigate the relationships between the history of abnormal pregnancy and the TORCH infections,and between the frequency of induced abortion and the TORCH infections in pregnant women.Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect TORCH infection in pregnant women with histories of abnormal pregnancies(study group) and normal pregnant women(control group).We also investigated the relationships between the frequency of induced abortion and the TORCH infections in pregnant women.Results (1) The rates of TORCH active infections in the study group were significantly higher than that of the control group(P0.05).Conclusions (1) TORCH series infection is one of the important causes of abnormal pregnant outcomes.(2) Women's Induced abortion is one of the risk factors in TORCH series infection.

Objective To observe the hemodynamic changes on right ventricular hypertrophy induced by monocrotaline in rats . Methods Male SD rats were given monocrotaline 60 mg/kg by a single intraperitoneal injection ,the mRNA expression of RV/BW , RVHI and ANF were took as the indicators .Intraperitoneal injection with 10% chloral hydrate was performed ,the artery cannulae and right ventricular catheter were inserted ,and the right ventricle pressure was measured .Results The RVHI and RV/BW were significantly increased in M2W and M4W groups ,compared with vehicle control (P<0 .01) ,and the ANF mRNA expression was significantly increased in both groups .Bp and HR did not change in model groups .RVEDP markedly decreased (P< 0 .01) and RVP ,± dp/dtmax ,t dp/dtmax and Vpm increased (P<0 .001) in M2W groups ;and all parameters increased significantly in M4W groups (P<0 .01) .Conclusion Monocrotaline can induce RVH ,which accompany hemodynamic changes and the systolic and dias‐tolic dysfunction in right ventricle may eventually cause right heart failure .

OBJECTIVE:To establish the method for the content determination of total saponins in Naomaitong.METHODS:Colormetric method was applied using panoxadiol as control.The detection wavelength was set at 556 nm.RESULTS:The linear range of panoxadiol was 2.03～10.15 ?g?mL-1(r=0.999 6) with an average recovery of 100.69%(RSD=1.76%,n=6).CONCLUSION:The method is easy to operate with high precision,accuracy and good reproducibility for the quality control of Naomaitong.

In order to improve the quality of teaching,stimulate students’ interest in studying medical cell biology,we performed a lot of innovation on cell biology teaching program according to the requirement of basal course and the characteristic of freshman. First,choose the contents of the study seriously. Second,introduce bilingual teaching gradually. Third,bring up the spirit of activity in their studies progressively. Last,take full advantage of tutorship to help students build up the studying confidence,master studying method and enhance self-study capability. The result shows that these methods help students conquer their fear of difficulties and achieved an expected teaching effect.

Objective To evaluate the influence of extraocular counterpulsation on ocular fundus blood vessels using high-frequency color Doppler ultrasonography. Methods Twenty-one patients with ischemic optic neuropathy were treated with extraocular counterpulsation. During treatment, the Doppler spectra were detected with high-frequency color Doppler ultrasonography. The peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance retinal index (RI) of central retinal artery and posterior ciliary artery were measured instantly before treatment and 1 min,5 min and 15 min after treatment. Results The instant PSV and EDV of central retinal artery and posterior ciliary artery were significantly increased (P＜0.05) after treatment, then decreased gradually. Fifteen min after treatment ,they returned to the level before treatment. Conclusions We can observe the changes of ocular fundus blood vessels noninvasively on high-frequency color Doppler ultrasonography. The method may be regarded as one of the basis for evaluating the effect of extraocular counterpulsation.

Objective To study the effect of psychological nursing on heart rate variability (HRV)in patients with acute myocardium infarction. Methods 80 patients with AMI were randomly divided into group A(routine nursing group)and group B (psychological nursing group) with 40 patients in each group,HRV was evaluated in both groups.The time domain and frequency domain of HRV of 24 h sinus rhythm in two groups of patients were analyzed. Results In group B,24 h average standard deviation of RR interval (SDNN),5 min segments of continuous average standard deviation of RR intervals (SDANN),the difference between adjacent cardiac RR interval of the root mean square (rMSSD),the percentage of adjacent canrdiac RR interval greater than 50 ms (PNN50),low frequency (LF),high frequency (HF) and other HRV parameters were significantly higher than group A. Conclusions Psychological nursing can significantly increase HRV and improve the electric stability of the ischemic myocardium.Psychological nursing can stabilize emotion of patients,decrease sympathetic tone,and may have a role in the prevention of sudden death.

Objective To investigate the relationship between sleep quality and mild cognitive impairment(MCI) in elderly patients with type 2 diabetes mellitus (T2DM). Methods We collected 97 elderly patients with type 2 diabetes mellitus and divided them into two groups: MCI group (DM-MIC) and normal cognition group (DM-NC group). All subjects were evaluated with cognition scores of Mini-mental state examination (MMSE), Montreal Cognitive Assessment (MOCA), Self-Rating Anxiety Scale(SAS), Center for Epidemiogical Studies Depression Scale (CES-D), Activity of daily living(ADL), Global Deterioration Scale(GDS), and Pittsburgh sleep quality index(PSQI). Independent t-test and Spearman's rank correlation analysis were performed. Results The hypnotic use in DM-MCI group was significantly more than that in DM-NC group (P = 0.046), and it had a negative correlation with the score of delayed recall, but not statistically significant(r= -0.277, P = 0.062). Significantly negative correlations were also found between the score of PSQI and MOCA (r= - 0.362), PSQI and attention(r= -0.319), PSQI and delayed recall (r= - 0.409), daytime dysfunction and MOCA (r= - 0.393), daytime dysfunction and abstraction (r= - 0.337), night sleep disorders and language (r= -0. 439), sleep efficiency and delayed recall (r = -0.325), and subjective sleep quality and delayed recall(r= -0.336) in DM-MCI group(P<0.05). Conclusion Sleep quality is correlated with some symptoms of mild cognitive impairment in elderly patients with T2DM.

Objective: To explore the effects of hydroxy-methyl-glutaryl coenzymeA reductase inhibitor (HMG-CoA RI) simvastatin on renal expression of apoptosis related gene Fas/Fas-L in aged diabetic rats after uninephrectomy. Methods: Elderly uninephrectomized SD rats were randomly divided into uninephrectomized control group (group C), streptozocin(STZ)-induced diabetic model group(group D), and diabetes + simvastatin group (group S). The apoptosis indices in renal tisses of three groups were detected by terminal deoxynucleotide mediated nick end labeling 12 weeks later. Immunofluorescence and real-time quantitative PCR were employed to observe the expression changes of Fas/Fas-L genes at the transcription and translation levels. Results: Compared with group C, group D had significantly higher apoptosis index and expression level of Fas/Fas-L; the above parameters were lower in group S compared with those in group D (P<0.05). The expression of Fas/Fas-L protein was mainly in the renal tubules. Conclusion: Both Fas and Fas-L genes are associated with the development and progression of diabetic nephropathy in aged rats. Simvastatin can down-regulate the translation and transcription of the genes, inhibit cell apoptosis and protect the kidney.

Objective To investigate the genetic diversity of variable number tandem repeats (VNTR) of Mycobacterium tuberculosis (M,ib) isolates and their impact factors on transmission.Methods A total of 1 310 patients with smear-positive pulmonary tuberculosis registered during 2013 from 30 counties of drug-resistance monitoring sites in Guangxi were enrolled.The VNTR genotyping technique was used to identify and analyze M.tb genotypes.Count data was analyzed by Chi-square test using descriptive statistical method,unconditioned Logistic regression was used to analyze the characteristics of gene clusters.Results A total of 1 310 strains of M.tb were classified into 964 genotypes,of which 779 strains were classified into a single gene cluster,531 strains were classified into 185 clusters.Each cluster contained 2 to 40 strains (mean 6.6).Patients aged between 41 and 60 years old had a highest gene cluster proportion (45.5％).Seven hundred and forty-six strains were Beijing genotype,while 564 were nonBeijing genotype,among which 7.4％ was original Beijing genotype.The estimated recent infection (cluster rate) was 26.41％.The cluster rate was higher in sensitive strains (24.40％) than in drugresistant strains (9.55 ％,x2 =23.621,P =0.000) and multi-drug resistant (MDR) strains (8.97 ％,x2=-9.675,P=0.002),and that was higher in Beijing genotype (28.69％) than Non-Beijing genotype (23.40％,x2 =4.610,P=0.032).The clusters proportion in sensitive strain (37.43％) was higher than drug-resistant strains (14.09％) and MDR strains (15.38％),and that was higher in Beijing genotype (40.88％) than Non-Beijing genotype (37.77％).Conclusions M.tb genetic polymorphisms display highly diversity in Guangxi region.Sensitive strains and Beijing genotype strains are the main epidemic strains.

Objective To evaluate the effects of endothelial nitric oxide synthase (eNOS) gene transfer on intrahepatic vascular resistance (IHVR) and portal venous pressure (PVP) in cirrhotic rats. Methods (1) 5 days after eNOS gene transfer, the in situ liver perfusion system (ISLP) was prepared and in different groups of controls and eNOS treated rats, the followings were analyzed: portal perfusion pressure (PP) dose-response curve to norepinephrine (NE); the effects on PP caused by specific nitric oxide synthase (NOS) inhibitor N-monomethyl-L- arginine (L-NMMA) or the nitric oxide (NO) synthesis substrate L-arginine (L-Arg). (2) The experiment of perfusion via portal vein in vivo was performed and the effects of L-NMMA on the PVP was observed. Results (1) In ISLP model, after L-NMMA was added into the perfusate of the control rats, PP dose-respose to NE increased remarkably and the peak of PP increased to (26.7?0.9) mm?Hg. The increased PP response to NE caused by L-NMMA was offsetted by L-Arg and the peak of PP decreased to (23.2?0.9) mm?Hg. In eNOS treated rats, PP response to NE was significantly lower than that in controls (P