The authors report a case of Von hippel Lindau disease in a 20 years old patients with presenting signs: diminution of visual acuity, intracramial hypertension with a left cerebollar syndrome and existence of an retinal angioma on the right side and papilla oedema on the left side. A scanning examination revealed a cystic tumor with a solid little mass. Angiography showed a capillary nodus coressponding to the mass lesion, with feeding arteries coming from the anterio superior cerebellar artery. Removal of the cyst and the tumor was successful. Pathological examination: Hemangi oblastoma. Negative ultrasound examination of the kidney, pancreas.
Hippel-Lindau Disease ; Case Reports [Publication Type]

Humans ; Minimally Invasive Surgical Procedures ; instrumentation ; methods ; Neurosurgical Procedures ; instrumentation ; methods

Anal Canal ; surgery ; Humans ; Postoperative Complications ; Rectal Neoplasms ; surgery

Female ; Humans ; Male ; Urogenital Abnormalities ; classification ; diagnosis ; Uterus ; abnormalities

Adolescent ; Adult ; Factor Analysis, Statistical ; Female ; Humans ; Male ; Middle Aged ; Needlestick Injuries ; epidemiology ; etiology ; Personnel, Hospital ; statistics & numerical data ; Retrospective Studies ; Risk Factors ; Young Adult

ABO blood group of 236 patients with aplastic anemia (AA) was compared with that of 2277 healthy individuals. Subjects with A antigen were susceptible to AA (R=1.3090, x~2=3.8489, P

Objective:To construct eukaryotic expression vectors by usingthep Silencer2.0 vector for inhibiting human livin gene by RNA interference,and to detect the effect of the silenced livin gene on the growth and apoptosis of U251 cells. Methods:Two target gene segments were synthesized and cloned into the pSilencer2.0 vectorrespectively to construct two recombinant eukaryotic expression vectors: pSilencer2.0-liv1 and pSilencer2.0-liv2,both of which were identified by enzyme digestion analysis. Then the U251 cells were transfected with the recombinant vectors and the interfering effects were detected by RT-PCR and Western blot. The proliferation of the U251 cells was detected by MTT method and their apoptosis index detected by flow cytometry. Results:The results of Enzyme digestion analysis showed that two target segments were cloned into pSilencer2.0 vectors. The results of RT-PCR and Western blot indicated that pSilencer2. 0-liv1 and pSilencer2.0-liv2 vectors could knock down the transcription and expression of livin gene,but the effect of pSilencer2.0-liv2 vectors was better than that of the pSilencer2.0-liv1 vectors. Obvious decrease in growth of the U251 cells was observed after transfected with pSilencer2.0-liv1 and pSilencer2.0-liv2 vectors,along with 17%increase of the apoptosis index in the U251 cells transfected with pSilencer2.0-liv2 vectors. Conclusion:The transcription and expression of livin gene were inhibited effectively in the glioma cell line U251. Aftertransfected with pSilencer2.0-liv2 vectors,the apoptosis index of the U251 cells was increased and the growth inhibited.

DRB alleles of 10 different HLA-DR individuals tested by serology typing were studied with polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, single-strand DNA of different sequence exhibited specific band patterns and mobilities by this technique. Our results suggested that PCR-SSCP is expected to be a useful tool for donor-recipient DRB allele HLA-matching in organ transplantation, as well as identification of criminal in forensic medicine.

Diabetes Mellitus ; diagnosis ; drug therapy ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hypoglycemic Agents ; therapeutic use ; Medicine, Chinese Traditional ; Phytotherapy ; Reference Standards

This paper reports the results of treating peridontal diseases with local irrigate on of matronidazole in five different concentrations. Comparative percentages of spirochets and coccal bacteria in pefridontal diseases were determined. It was found that metronidazole in concentrations over 0.05% were effective in treating of simple periodontitis, necrotic gingivitis, pregnant gingivitis and marginal gingivitis cet. This methoa is useful for those who can not be adminished with oral route.