Objective To discuss the clinical value of laparoscopic myomectomy.Methods We compared clinical data of laparoscopic myomectomy(Laparoscopic Group,n=185) with open myomectomy(Open Group,n=69),from December 2002 to February 2005,in respect of operative time,hemorrhage volume,time to normal temperature,recovery time of bowel movement,and duration of hospital stay.Results Conversions to open surgery were required in 2 cases in the Laparoscopic Group because of difficulties of hemostasis.There was no significant difference in the operative time(t=1.849,P=0.066) between the Laparoscopic Group(81.3?14.7 min) and the Open Group(77.2?18.1 min).The intraoperative hemorrhage volume was significantly less in the Laparoscopic Group(101.5?36.7 ml) than in the Open Group(154.5?61.1 ml)(t=-8.397,P=0.000).Shorter time to normal temperature,recovery time of bowel movement,and hospital stay were achieved in the Laparoscopic Group(2.7?0.8 d;13.8?5.4 d;6.0?1.5 d) than in the Open Group(3.8?1.0 d;23.3?6.0 d;8.0?2.0 d),with significant difference(t=-9.064,P=0.000;t=-12.074,P=0.000;t=-8.575,P=0.000).Follow-ups were conducted in 152 cases in the Laparoscopic Group for 13.2?8.7 months,which revealed 17 cases of recurrence(11.2%) and 17 cases of pregnancy within 1 year out of 57 cases of infertility(29.8%).As compared with the Laparoscopic Group,followups were conducted in 48 cases in the Open Group for(12.5?)7.9 months(t=0.511,P=0.610),which revealed 8 cases of recurrence(16.7%;?~2=1.003,P=0.317) and 4 cases of pregnancy within 1 year out of 16 cases of infertility(25.0%;?~2=0.004,P=0.949).Conclusions Laparoscopic myomectomy has advantages of micro-invasion,little blood loss,rapid postoperative recovery,and short hospitalization.However,it cannot entirely supersede the open myomectomy.

G mutation were intervened and avoided the occurrence of deafness,1 babies with 235delC homozygote was confirmed severe sensorineural hearing loss in the hearing screening.Conclusion Newborn gene screening make up the defects of missed diagnosis in simple hearing screening in finding the newborn babies with late-onset deafness or the high risk as well as the pathogenic carriers.So the hearing and gene screening were necessary in the current situation,and this screening strategy would be developed further in Henan province.

OBJECTIVETo investigate and explain the composing mechanism by observing enhancement property of disassembled compositions of Yiqi Huoxue decoction (YQHXD) on platelet aggregation of health adults.

METHODVenous whole blood was obtained from healthy human subjects and anti-coagulated, and then centrifuged to produce platelet-rich plasma (PRP) and platelet-poor plasma (PPP). Disassembled compositions of YQHXD and sodium chloride were placed in different translucent tubes containing PRP and PPP, calculated the maximum percents of platelet aggregation stimulated by platelet agonists: adenosine diphosphate (ADP), platelet activating factor (PAF) and arachidonic acid (AA).

RESULTThe maximum percents of platelet aggregation induced by ADP and PAF were largely improved by YQHXD, both astragalus membranaceus and Angelica Scinesis played the most important roles in the decoction, the herbs of Yiqi and Huoxue must work together to enhance platelet aggregation.

CONCLUSIONIn vitro study, YQHXD can markedly improve platelet aggregation of health adults, but the exact mechanism should be proved by further experiments.

Adenosine Diphosphate ; pharmacology ; Adult ; Angelica sinensis ; chemistry ; Arachidonic Acid ; pharmacology ; Astragalus membranaceus ; chemistry ; Drug Combinations ; Drug Synergism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Humans ; Male ; Middle Aged ; Plants, Medicinal ; chemistry ; Platelet Activating Factor ; pharmacology ; Platelet Aggregation ; drug effects ; Young Adult

Animals ; Blood Coagulation Tests ; Liver ; blood supply ; metabolism ; Male ; NF-kappa B ; biosynthesis ; genetics ; Peroxidase ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism ; Thromboplastin ; biosynthesis ; genetics

Taking the Maternal and Child Health Care Hospital of Zhenjiang as an example,the paper introduces its mobile medical insurance payment system based on the Internet payment platforms,describes the system architecture and the main functional modules including binding of social security card,diagnosis and treatment service,personal center and so on,and analyzes the existing problems and deficiencies.

Objective To explore the application effect of nosting barrier film in the skin nursing of laryngeal carcinoma patients with trachea cannula after surgery.MethodsTotally 90 cases of laryngeal cancer patients after radiotherapy with tracheal cannula,were randomly divided into three groups,with 28 cases in the control group,33 cases in the dressing group,and 29 cases in the skin protective film group. Patients in the control group were treated with basic nursing method including using saline to clean plastic casing,cleaning and disinfecting skin,using sterile gauze to cover the field of radiation skin and plaster fixation;dressing group adopted Comfeel hydrocolloid dressings on the basis of basic nursing method;skin protective film group adopted Genetime spray skin protective film to protect skin of radiation field on the basis of basic nursing. After radiotherapy,the incidence of skin injury,severity of skin injury,patient comfort,and the time and times of changing skin dressing everyday were observed and compared among three groups.Results The incidence of radiation skin injury in the skin protective film group (6.89%) and dressing group (15.15%),were all lower than the control group (50%) (χ2=16.75,P<0.05);the times of changing skin dressing everyday in the skin protective film group (4.28±0.53) times/d and the dressing group (4.52±0.76) times/d,were lower than the control group (6.04±1.35) times/d (Hc=32.65,P<0.05);patient comfort in the skin protective film group and the dressing group was superior to the control group (Hc=22.42,P<0.05);the time of changing skin dressing in the skin protective film group (10.40±1.35) min,was less than the dressing group (14.09±1.27) and the control group (12.35±1.28) min (F=85.87,P<0.05).Conclusions The skin protective film can effectively reduce the incidence of skin injury in patients with laryngeal cancer and radiotherapy,improve patient comfort,reduce the labor time of nurses,and improve the quality of care and efficiency.

OBJECTIVE: This study was performed to evaluate the results of conization by loop electrosurgical excision procedure (LEEP) and cold coagulation as a definitive treatment in the patients with FIGO stage Ia1 squamous cell carcinoma of the cervix. METHODS: One hundred eighty-seven patients were diagnosed as stage Ia1 cervical squamous cell carcinomas from 1995 to 2004 by conization with LEEP and cold coagulation. Fifty-nine patients who wanted to preserve fertility and/or refused further surgical treatment were followed-up without further treatment. Eleven patients of the 59 had involved ectocervical resection margins. All patients were followed-up with cervicovaginal smear and colposcopic examination at a regular interval. Disease recurrence was defined as a histologic diagnosis of dysplasia or more. RESULTS: The median follow-up period was 69.0 months (range 8 to 103). All 59 patients had no lymphvascular space invasion (LVSI). In four patients, the ectocervical margins were involved by dysplasia, in seven patients, by carcinoma in-situ. There were no specific differences in ages, depth of stromal invasion and HPV status between the groups with and without involved margins. All 59 patients did not recur during follow-up period. CONCLUSION: Conization with LEEP and cold coagulation was feasible and could be used as a definitive therapy for the patients with stage Ia1 cervical squamous cell carcinoma. This study suggests that conization might play a role in a patient with positive margins (dysplasia or CIS) when LVSI is not demonstrated.
Carcinoma, Squamous Cell* ; Cervix Uteri* ; Conization* ; Diagnosis ; Female ; Fertility ; Follow-Up Studies ; Humans ; Recurrence

OBJECTIVETo investigate the telomerase activity in mesenchymal stem cells (hMSCs) from human bone marrow after their in vitro committed differentiation into adipocytes and cryopreservation.

METHODShMSCs were isolated from human bone marrow. The isolated hMSCs were induced to differentiate into adipocytes in vitro or cryopreserved. TRAP assay (telomerase repeat amplification protocol assay) was employed to detect telomerase activity in those hMSCs.

RESULTSTelomerase activity (RTA) in hMSCs (n=19) was (1.46 +/-0.67)%, while that in hMSCs-derived adipocytes (n=3) was (11.80 +/-2.52)% (P<0.001). RTA of hMSCs-passage 1.3 (n=10) was (1.46+/-0.83)%, and that of hMSCs-passage 4-7(n=9) was (1.46 +/-0.47)% (P=0.99). Cryopreservation did not affect the telomerase activity in hMSCs, RTA of fresh hMSCs (n=13) was (1.41 +/-0.44)%, RTA of frozen hMSCs (n=6) was (1.57 +/-1.07)% (P=0.64).

CONCLUSIONhMSCs are telomerase-negative, but telomerase activity in hMSCs-derived adipocytes is upregulated.

Adipocytes ; cytology ; enzymology ; Bone Marrow Cells ; cytology ; enzymology ; Cell Differentiation ; Cells, Cultured ; Cryopreservation ; Humans ; Mesenchymal Stromal Cells ; cytology ; enzymology ; Telomerase ; metabolism

OBJECTIVETo observe the distribution pattern of human telomere repeat binding factor 1(TRF1) in the telomerase-positive (HeLa) and telomerase-negative cells (WI38-2RA) and to investigate its expression level during the cell cycle.

METHODSThe full-length sequences of TRF1(TRF1FL) and its mutant with N and C terminus deletion (TRF1DeltaNC) were generated by PCR amplification, the resulting fragments were cloned into pEGFP-C2 mammalian expression vector. GFP-tagged proteins were verified by Western blotting with rabbit anti-TRF1 and mouse anti-GFP antibodies after cell transfection. Immunofluorescence staining were performed to detect the TRF1 localization in HeLa and WI38-2RA cells. Metaphase spreads from HeLa cells were also prepared to observe TRF1 localization in chromosomes. HeLa cells were arrested by thymidine and nocodazole at different cell stages. Cell cycles were analyzed by flow cytometry and TRF1 levels were evaluated by semi-quantitative Western blotting.

RESULTSTRF1FL and TRF1PNC fragments were sized about 1.3 kb and 0.95 kb. GFP-tagged TRF1FL and TRF1DeltaNC proteins were 80 kD and 60 kD, respectively. In both HeLa and WI38-2RA cells, TRF1FL had a speckled distribution in the nuclei,however, TRF1FL did not coincide with promyelocytic leukemia (PML) nuclear body in HeLa cells while it exclusively did in WI38-2RA cells. Moreover, TRF1FL was exactly localized at the termini of metaphase spreads in HeLa cells. In contrast, TRF1PNC was diffusely distributed throughout the nuclei. Analysis by semi-quantitative Western blotting indicated that TRF1 levels increased with cell cycle progression, which reached the zenith at the M phase and went down to the nadir at G1/S point. The TRF1 level at M phase was about 3.9 times than that at G1/S point(t=12.92iP<0.01).

CONCLUSIONTRF1 has a different localization in telomerase-positive and telomerase-negative cells, which suggests TRF1 might exert different functions in these cells. TRF1 level is regulated with cell cycle.

Cell Cycle ; HeLa Cells ; Humans ; Leukemia, Promyelocytic, Acute ; pathology ; Mutation ; Telomerase ; metabolism ; Telomere-Binding Proteins ; biosynthesis ; genetics ; metabolism ; Telomeric Repeat Binding Protein 1 ; biosynthesis ; genetics ; metabolism ; Tumor Cells, Cultured

Palatal radicular groove leads to severe endo-periodontal lesion in lateral incisors. The groove occurs on the labial and lingual root surfaces, while the localized periodontal pocket develops along the lingual groove length. The complicated root canal system, extensively destroyed bone lamella, and persistent fistula pose a challenge for root canal therapy, tooth retention, and thorough infection control inside and outside of the root canal. Combining microscopic root canal therapy with intentional replantation and nano-biomaterial application facilitates infection control, tooth retention, and formation of newly periodontal attachment.