OBJECTIVE:To observe the effect of ligustrazin on the mitochondrial damage of myocardium in ischemia-reperfusion rats and the mechanism involved METHODS:Rat model of myocardial ischemia-reperfusion injury was established by coronary artery ligation and the activities of Ca2+-ATPase,Ca2+?Mg2+-ATPase,succinic dehydrogenase(SDH),cytochrome oxidase(CCO),SOD,GSH-PX and contents of phospholipid(PL),MDA,Cyt aa3 and Cyt C in the myocardial mitochondria were observed RESULTS:As compared with ischemia-reperfusion group(IR),IR+L(ligustrazin)group showed significant increase in the activities of Ca2+-ATPase,Ca2+?Mg2+-ATPase,SDH,CCO,SOD and GSH-PX(P

AIM: To observe the dysfunciton of myocardial nuclear calcium transport in rat myocardial injury and the effects of liqustraxin on it. METHODS: The model of myocardial damage was induced by subcutaneous injection of isoproterenol (ISO,5 mg?kg -1?d -1). Myocardial nuclei were purified with sucrose density centrifugation and identified zymologically. The activity of Ca 2+-ATPase was measured zymologically and calcium uptake was assayed with 45Ca 2+ isotope. RESULTS: Compared with the control,the Ca 2+-ATPase activity of myocardial nuclear membrane in ischemia group(exprimental) was decreased by 18.1% (P

With the appearance of direct-acting antivirals (DAAs), breakthroughs have been achieved in the antiviral therapy for hepatitis C and hepatitis C patients can be cured completely. Due to the insidious onset of hepatitis C, most patients do not know their own conditions, and thus expanding diagnosis and treatment through screening is the key to the elimination of hepatitis C. However, hepatitis C virus is distributed widely and unevenly in the world, which results in difficulties in the screening and diagnosis of hepatitis C. This article introduces the current economic research on hepatitis C screening, analyzes the factors affecting the cost-effectiveness of hepatitis C screening, and shares the strategies and advances for hepatitis C elimination in other countries, so as to provide a reference for eliminating hepatitis C in China.