1.Simultaneous Determination of Six Components in Xiao'er Kechuanling Granule by HPLC
Zebing ZHU ; Dewei ZHANG ; Lamei MOU ; Ya GUO
China Pharmacist 2018;21(2):354-357
Objective:To establish an HPLC method to determine the contents of ephedrine hydrochloride, (R, S)-goitrin, lae-trile,chlorogenic acid,licorice glycosides and glycyrrhizic acid in Xiao'er Kechuanling granule.Methods:The chromatography condi-tions were as follows:an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column was used,and acetonitrile-0.1% phosphoric acid was applied as the mobile phase. The flow rate was 1.0 ml·min-1with gradient elution. The wavelength was 207 nm for ephed-rine hydrochloride and laetrile,237 nm for glycyrrhizic acid,licorice glycosides and chlorogenic acid and 245 nm for (R,S)-goitrin. Results:The linear range of ephedrine hydrochloride was 2.423-96.920 μg·ml-1(r =0.999 2), and the average recovery was 100.1% (RSD=0.30%,n=6). The linear range of (R, S) -goitrin was 1.920-76.798 μg·ml-1(r=0.999 9), and the average recovery was 99.86% (RSD=1.14%,n=6). The linear range of chlorogenic acid was 2.396-92.891 μg·ml-1(r=0.999 9),and the average recovery was 98.57% (RSD =0. 75%,n =6). The linear range of amygdalin was 1.982-79.279 μg·ml-1(r =0.999 8),and the average recovery was 99.67% (RSD=0.59%,n=6). The linear range of glycyrrhizin was 2.136-85.440 μg· ml-1(r=0.999 9),and the average recovery was 98.57% (RSD=0.69%,n=6). The linear range of glycyrrhizic acid was 2.432-97.260μg·ml-1(r=0.999 9),and the average recovery was 98.57% (RSD=0.11%,n=6). Conclusion: The method is accu-rate and reliable,which can be used for the quality control of Xiao'er Kechuanling granule.
2.Determination of Six Heavy Metals in Iron Filings and Ferric Ammonium Citrate by Atomic Absorption Spectrophotometry
Zebing ZHU ; Dewei ZHANG ; Lamei MOU ; Jie KANG
China Pharmacist 2018;21(3):512-515
Objective:To establish an atomic absorption spectrophotometric method for the determination of 6 heavy elements in-cluding lead,cadmium,mercury, arsenic, copper and chromium in iron filings and ferric ammonium citrate in order to control the quality and ensure people's daily medicine safety.Methods:The determination method for lead,cadmium,chromium,copper,arse-nic and mercury was a graphite furnace method, a graphite furnace method, a graphite furnace method, a flame method, a hydride method and a cold steam absorption method,respectively. Results:The concentration of lead within the range of 0-50 ng·ml-1had a good linear relationship with the absorbance(r=0.999 8),and the average recovery was 98.9%(RSD=2.3%,n=6). The concen-tration of cadmium within the range of 0-4 ng·ml-1had a good linear relationship with the absorbance(r=0.999 1),and the average recovery was 90.5%(RSD=1.5%,n=6). The concentration of chromium within the range of 0-50 ng·ml-1had a good linear rela-tionship with the absorbance(r=0.999 7),and the average recovery was 96.1%(RSD=1.1%,n=6). The concentration of copper within the range of 0-800 ng·ml-1had a ood linear relationship with the absorbance (r=0.999 9), and the average recovery was 97.5%(RSD=1.2%,n=6). The concentration of arsenic within the range of 0-16 ng·ml-1had a good linear relationship with the absorbance (r=0.999 0),and the average recovery was 93.4%(RSD =1.1%, n =6). The concentration of mercury within the range of 0-18 ng·ml-1had a good linear relationship with the absorbance(r=0.999 2),and the average recovery was 93.2%(RSD=3.8%,n=6). Conclusion:The method with good repeatability is accurate,which can be used for the quality control of iron filings and ferric ammonium citrate.