Objective To explore the cleaning effect of moisturizing through multiple enzymes cleaning fluid or alkaline cleaning fluid in the cleaning of medical equipment .Methods One thousand and two hundred surgical instruments used by cesarean delivery and recycled immediately from June 2013 to September 2013 were chosen and divided into the Ⅰ,Ⅱ,Ⅲ,Ⅳgroup according to the random number table , each with 300 .the ⅠandⅡgroup received the cleaning 2, 12 h after the moisturizing through the multiple enzymes cleaning fluid , respectively;and Ⅲ and Ⅳ group received the cleaning 2, 12 h after the moisturizing through the alkaline cleaning fluid , respectively .The qualification rates of cleaning were compared among the groups .Results No differences were found in the qualification rates of cleaning through the visual , microscopically and test paper detection between Ⅰ and Ⅲ groups ( P >0.05 ).The qualification rates of cleaning through the visual , microscopically and test paper detection were respectively 97.33% (292/300),96.33% (289/300) and 97.00%(291/300) in Ⅳ group, and were higher than 87.00% (261/300),86.33% (259/300) and 86.33%(259/300) in Ⅱ group, and the differences were statistically significant (χ2 =18.690,18.950, 14.592, respectively;P<0.01).The qualification rates of cleaning through the visual , microscopically and test paper detection were respectively 98.67% (296/300),98.00% (294/300) and 98.33% (295/300) in Ⅰgroup, and were higher than those of Ⅱgroup, and the differences were statistically significant (χ2 =30.688, 22.017,30.513, respectively;P <0.01).No differences were found in the qualification rates of cleaning betweenⅢandⅣgroups (P>0.05).Conclusions The surgical instruments after the normal use should be cleaned within 2 hours, and the moisturizing through multiple enzymes cleaning fluid or alkaline cleaning fluid can all ensure the cleaning quality .The surgical instruments after the use can not be cleaned within 2 hours, and should be firstly moisturized through the alkaline cleaning fluid and secondly cleaned through the machine so as to improve the cleaning quality of instruments .