Objective: To study NOS activities in serum of rats under different stresses. Methods: Two models of stress were made in rats, one is the conventional forced swimming model (including 12 rats), and the other is a social defeated paradigm (including 12 rats). The activities of NOS of rats under each model and of one control group (10 rats) were measured. Results: Compared with control, the NOS activity under social defeated paradigm was lower (15.36?1.74/17.11?1.67 U/ml, t=2.32, P

Objective: To study the change of NO (nitric oxide) and NOS (nitric oxide synthase) in brain of rats induced by acute stress Method: The concentration of NO and NOS in frontal lob, hippcampus, midbrain and hypothalamus were measured in 1 and 3 hours after stress respectively Results: The concentration of NO and NOS in hippcampus and hypothalamus were significantly higher than that of control group at 1 hour after stress (P

Objective:To examine the effects of psychological and physiological stress on hippocampal extracellular monoamine content under simulated high-altitude hypobaric hypoxia.Methods:The male SD rats were randomly divided into ten groups:control(Con group), hypoxia(Hy group), psychological stress(twice/day)(Ps1group), psychological stress(twice/day?2day)(Ps2 group), psychological stress(twice/day) + hypoxia(Ps1+Hy group), psychological stress(twice/day? 2day) +hypoxia(Ps2+Hy group), physiological stress(twice/day)(Ph1group), physiological stress(twice/day?2day)(Ph2 group), physiological stress(twice/day) + hypoxia(Ph1+Hy group), physiological stress(twice/day?2day) + hypoxia(Ph2+Hy group).The contents of norepinephrine(NE), dopamine(DA) and 5-hydroxytryptamine(5-HT) in extracellular fluid of hippocampus collected by push-pull perfusion were determined by high performance liquid chromatography with electrichemical detection(HPLC-ECD).Results:①Compared with the Con group, the content of NE was significantly lower in Hy, Ps1+Hy and Ph2+Hy groups(P

Objective:To investigate the effect of cold stress (-10℃?10h) on NO and NOS in brain of mice. Methods: Forty mice were assigned into two groups randomly: The experimental group was the stress group and the other was the control group. The levels of NO and NOS activity were determined after cold stress. Results:The level of NO in stress group was significant lower than that in control group ( t =2.67,P

Objective: To explore the brain levels of nitric oxide (NO) and nitric oxide synthase(NOS) in rats after sleep deprivation. Methods: NO levels and NOS activity were assayed in different brain regions of Sprague-Dawley rats after sleep deprivation by flower pot compare with normal group (CC) and tank control groups (TC). Results: NO levels and NOS activity were increased in front cortex and hippocampus after SD compared with CC and TC (P

Objective: To explore the stress effects of traffic accident on changing the NO level and SOD activity, as well as the relationship between personality and prognostic change. Methods: Serum level of NO and activity of SOD were measured in 152 patients with traffic accidents and in 60 control subjects. All subjects were assessed with the Eysenck Personality Questionnaire (EPQ). Results: NO and SOD were significantly higher in patients as compared to controls ( P

Objective:To explore the 5-HT mechanism of stress . Methods:The method of polymerase chain reaction (PCR) was used to detect the presence of 5-HT receptor subtypes in the hippocampus,hypothalamic and midbrain of the rat after 30days of movement restrict compared with normal cage control groups (CC) . The specific oligonucleotide primers were synthesized based on the complementary DNA sequence for each of the rat 5-HT 1A and 5-HT 2A receptor subtypes . Results : The expression of 5-HT 1A receptor subtypes in each regions of the rat brain after movement restrict were signifcantly lower than that of the CC groups, and the expression of 5-HT 2A receptor subtype were signifcantly higher( P

Objective: To investigate the influence of live ammunition maneuver on mental health of soldiers in mobile troop. Method: 428 soldiers in mobile troop participating live ammunition maneuver on plateau were included. 198 soldiers in another troop garrisoned on plateau were collected as control. SCL-90, EPQ and SSRS (social support rating scale) were applied to soldiers in mobile troop before and after ammunition, and also to controls. Results: After ammunition, numbers of positive items and all 9 subscores of SCL-90 increased than before (P

Objective: To study the rate of PTSD after traffic accident in orthopedics and do retrospective analysis. Method: Eighty one patients wounded by traffic accident in orthopedic ward were surveyed. Acute stress reaction (ASR) and posttraumatic stress disorder (PTSD) were diagnosed according to CCMD-2-R criteria. SCL-90 and EPQ were also applied. Results: 33 cases (40.7%) were diagnosed as ASR and 31 (38.3%) as PTSD among all subjects. SCL-90 score and nearly all subscores were higher in patients with PTSD than patients without (P

Objective: To study the changes of serum interleukin levels and immune function of rats under stress. Method: 18 male rats were randomly divided into two groups, stress group (10) received "psychosocial"stress and their serum were taken after 1 hour; control group (8). Serum interleukin 2, 6, 8 and cortisol levels of all subjects were determined. Results: Compared with control group, the level of serum cortisol increased after stress (6.51?2.5/3.76?0.4 (ug/dl, t=3.45, P