3.Early outcome of peripheral cutting balloon in the treatment of peripheral vessel stenosis
Jin YANG ; Lu-Bin LI ; Jin-Hua MEI ; Yue-Feng ZHU ; Lai-Gen SHEN ;
Chinese Journal of Radiology 2001;0(07):-
Objective To investigate the early clinical outcome of peripheral cutting balloon(PCB) in the management of peripheral vessel stenosis.Methods Thirteen patients with peripheral limb vessel stenosis, in which 4 stenoses in hemodialysis access and 9 at lower limb arteries,underwent angioplasty by PCB.For multiple stenosis in the same vessel,the distal one should be expanded firstly.The balloon pressure was controlled in the range of 8 atm to 10 atm(1 atm=10.108 kPa).All the patient were given continuous anticoagulant therapy after the procedure.Results All the procedure were carried out successfully on the 13 patients,and no serious complications occured.The symptoms did not recur in all patients after the procedure.The 5 months' follow-up angiography proved that no restenosis occurred in one patient with previous stenosis at the hemodialysis access.Conclusion The angioplasty with PCB was a safe and reliable procedure in management of the peripheral limb vessel stenosis.The early outcome is satisfying.
4.Preparation of monoclonal antibodies against human mesenchymal stem cells.
Zhi-xiong LIAO ; Jian-gen SHEN ; Xiao-yu LAI
Journal of Zhejiang University. Medical sciences 2006;35(5):517-522
OBJECTIVETo prepare monoclonal antibodies (McAbs) against human mesenchymal stem cells (hMSCs) and to study their biological characteristics.
METHODSBALB/C mice were immunized with pooled hMSCs. McAbs were prepared by hybridoma technique and their biological characteristics were analyzed by indirect immunofluorescence, immunohistochemistry and flow cytometry.
RESULTFive hybridoma cell lines were successfully established, which secret McAbs specifically against hMSCs. Investigations showed that all these McAb reacted only to hMSCs and had no cross-reaction to other human cells, the relative affinities of 5 McAbs were 1x10(6) (ZUB1), 1x10(5) (ZUB4), 1x10(6) (ZUC3), ZUE12 (1x10(5)) and 1x10(5) (ZUF10), respectively. Isotype analysis showed that ZUB1, ZUE12, ZUF10 against the same isotype, while ZUC3, ZUB4 against other two different isotypes alone. Flow cytometric analysis showed that the positive expression rate of cultured hMSCs was 87.39% (ZUB1), 88.07% (ZUB4), 88.12% (ZUC3), 69.89% (ZUE12) and 83.67% (ZUF10).
CONCLUSIONThe prepared five McAbs can specifically react against hMSCs, which can be used for selection and study of hMCSs.
Animals ; Antibodies, Monoclonal ; biosynthesis ; Antibody Specificity ; Bone Marrow Cells ; cytology ; immunology ; Fluorescent Antibody Technique ; methods ; HL-60 Cells ; Humans ; Hybridomas ; secretion ; Immunoglobulin G ; immunology ; Immunoglobulin M ; immunology ; Mesenchymal Stromal Cells ; cytology ; immunology ; Mice ; Mice, Inbred BALB C ; Rats
6.Surgical treatment of annular pancreas in adults: a report.
He-Ming ZHENG ; Xiu-Jun CAI ; Lai-Gen SHEN ; Robert FINLEY
Chinese Medical Journal 2007;120(8):724-725
Abdominal Pain
;
diagnosis
;
etiology
;
surgery
;
Adult
;
Duodenal Obstruction
;
complications
;
diagnosis
;
surgery
;
Humans
;
Male
;
Pancreas
;
abnormalities
;
surgery
;
Tomography, X-Ray Computed
;
Treatment Outcome
8.Surgical treatment of renal cell carcinoma metastasized to the duodenum.
Jin YANG ; Yuan-Biao ZHANG ; Zhen-Jie LIU ; Yue-Feng ZHU ; Lai-Gen SHEN
Chinese Medical Journal 2012;125(17):3198-3200
Aged
;
Carcinoma, Renal Cell
;
pathology
;
secondary
;
Duodenal Neoplasms
;
secondary
;
surgery
;
Female
;
Humans
;
Kidney Neoplasms
;
pathology
;
Nephrectomy
9.Comparison of effects between China made sirolimus-eluting stents and bare stents for treating patients with acute myocardial infarction.
Hui-gen JIN ; Zong-jun LIU ; Wei YANG ; Wei-qing WANG ; Jia SHI ; Dong-yi WANG ; Zhi-hua WANG ; Hong-mei YU ; Ying LAI ; Jun SHEN ; Ping FANG
Chinese Journal of Cardiology 2007;35(8):697-700
OBJECTIVETo compare the clinical outcomes between China made sirolimus-eluting stents (SES) and bare metal stents (BMS) implantation in patients with acute myocardial infarction (AMI).
METHODSConsecutive patients with AMI underwent primary percutaneous coronary intervention (PCI) were randomly divided into SES group (n = 87) and BMS group (n = 86). The incidence of major adverse cardiac events (MACE including death, reinfarction, in-stent thrombosis, restenosis rate, target vessel revascularization) up to 6 months post PCI were assessed.
RESULTSPostprocedure vessel patency, enzymatic release, cardiac function, and the incidence of short-term MACE were similar between the two groups (all P > 0.05). Two in-stent thrombosis was diagnosed in the SES group and bare stents group respectively (2.4% vs. 2.3%, P > 0.05). At 6 months, In-stent restenosis rate (4.5% vs. 40.0%, P < 0.01) and the in-segment restenosis rate (6.8% vs. 44.9%, P < 0.01) as well as MACE (8.0% vs. 24.4%, P < 0.01), which is mainly due to a marked reduction in the risk of target vessel revascularization (3.4% vs. 11.6%, P < 0.05) were significantly lower in SES group compared to BMS group.
CONCLUSIONThe China made SES were not associated with an increased risk of in-stent thrombosis but significantly reduced restenosis rate and MACE at 6 months post primary angioplasty in patients with AMI.
Aged ; Angioplasty, Balloon, Coronary ; Drug-Eluting Stents ; Female ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; therapy ; Sirolimus ; administration & dosage
10.Predictive factors for conversion of transurethral resection of the prostate to open prostatectomy.
Guo-Dong LIAO ; Zhou-Jun SHEN ; Xie-Lai ZHOU ; Shan-Wen CHEN ; Jun CHEN ; Shuo WANG ; Shi-Fang SHI ; Zhi-Gen ZHANG ; Song-Liang CAI
National Journal of Andrology 2007;13(7):613-616
OBJECTIVETo evaluate the factors for the conversion of transurethral resection of the prostate (TURP) to open prostatectomy and to provide clinical evidence for surgical options.
METHODSFrom January 1997 to March 2005, we performed 1 086 TURP and made retrospective analyses of 11 risk factors concerning the demographics, clinical history, laboratory data, ultrasound results, and intraoperative complications of the patients. In addition, multivariate logistic regression was used to determine those variables predicting the conversion of TURP.
RESULTSThirty-nine (3.59%) of the TURP cases required conversion, mostly because of uncontrollable hemorrhage (71.79%). Multivariate analyses showed that a prostate volume > 85.2 ml (OR = 2.568, P < 0.01), intraoperative slit of capsula prostatic (OR = 1.916, P < 0.01) and a second midstream bladder specimen (VB2) white blood cell count of the urine > 13.5/HP (OR = 1.486, P < 0.01) predicted the conversion to open prostatectomy.
CONCLUSIONBenign prostatic hyperplasia (BPH) patients with a huge prostate and those with intraoperative slit of capsula prostatic undergoing TURP are more likely to be converted. And uncontrollable hemorrhage, huge prostate and poor endoscopic vision are the major reasons for the conversion.
Aged ; Aged, 80 and over ; Humans ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Postoperative Hemorrhage ; etiology ; Prostatectomy ; methods ; Prostatic Hyperplasia ; surgery ; Retrospective Studies ; Risk Factors ; Transurethral Resection of Prostate ; adverse effects ; methods ; Treatment Outcome