Let-7 is found to be downregulated in lung cancer while many kinds of oncogenes expression always increase, and restoration of the let-7 to normal expression level inhibits lung cancer growth evidently.Thus, let-7 might serves as good targets or effective interference tools in exploration of anticancer therapeutics,and has a good prospect in applying it in the early diagnosis, prognosis and therapy in lung cancer.

Mitochondria are important intracellular energy supply organelles .As semi-autonomous organelles , the mitochondrial biogenesis , damage and clearance were the dynamic processes , which are dual-regulated by mitochondrial genes and nuclear genes , and maintain mitochondrial homeostasis according to the needs of the cells for energy .Recent studies provide evidence that the disorder of mitochondrial biogenesis in the neurons participates in the pathological process after cerebral ischemia/reperfusion, resulting in metabolic disturbance and cell apoptosis .This paper reviews the research progress of mitochondrion and cerebral ischemia/reperfusion injury .

Objective:To investigate levels of serum IL-34 in SLE patients,and correlation between concentrations of serum IL-34 and other established serum markers and disease activity indexes.Methods: In all,78 SLE patients and 53 healthy controls were enrolled in the research.Enzyme-linked immunosorbent assay(ELISA) was employed to measuring the concentrations of serological IL-34.Then,serum IL-34 levels between SLE group and healthy controls were analyzed by Mann-Whitney U test.Meanwhile,the correlation between the serum IL-34 levels and disease activity indexes and other established serum markers were assessed.Serum IL-34 levels were significantly higher in SLE patients compared to healthy controls[(Median,128.9 pg/ml) vs (Median,52.4 pg/ml),P<0.001].Results: Their levels were remarkably associated with accumulation of the clinical features of SLE.Additionally,IL-34 titers were positively correlated with the SLE disease activity indexes,anti-double stranded DNA antibody(anti-dsDNA)titers and C-reactive protein(CRP)levels,but inversely with C3 levels.Conclusion: Serum IL-34 could be a candidate biomarker for SLE as the elevated serum levels in treatment-naive SLE patients and its association with SLE disease activity.

Objective To study the use on curative effect of Chinese medicine Qingyitang in severe acute pancreatitis.Methods 132 cases of severe acute pancreatitis were randomly divided into 2 groups,The control group(n=62) and the treatment group(n=70).Routine treatment were given in both,otherwise,the Chinese medicine Qingyitang was used in the treatment group.The operation rate,the infection rate,the time of abdominal distension removing and fasting and being hospitalized were observed and compared between two groups.Results Every index of the treatment group was finer than the control group.Conclusions The Chinese medicine Qingyitang showes their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disturbance following acute pancreatitis.As a result,the chances of bacterial translocation and enterogenic infection declines.These preparation might raise the treatment effects,as a well,shorten the course of treatment.

Objective To study the expression of p27 gene in endometrial carcinoma and its clinical significance.Methods By using streptavidin-biotin peroxidase(SP) method the expression of p27 gene were examined in 20 normal endometrial tissues and 65 endometrial carcinoma tissues.Results p27 was expressed in 33 of 65(51%) cases of endometrial carcinomas.In contrast,p27 was all expressed in normal endometrial tissues.The expression of p27 was related to the pathological grades,clinical stages and depth of myometrial invasions.Conclusion The level of p27 expression is closely related to the growth,invasion and differentiation of endometrial carcinoma.

The adhesion molecules expressed by brain microvascular endothelial cell maintain the structure and function of brain, ensure the continuity of endothelium cell and penetrate blood-brain barrier in physiology. They also intervene inflammation cells that remove or orientate regular or inflammation organism in pathology. The adhesion molecules of Immunoglobulin supperfamily and Selectin family are expressed by brain microvascular endothelial cell and have affinity with the ischemic brain damage. The advance in research on relationship between the expression of adhesion molecules by brain microvascular endothelial cell and ischemic brain damage is reviewed in this article.

Animals ; Electrophoresis, Gel, Two-Dimensional ; methods ; Gene Expression Profiling ; Humans ; Neoplasm Proteins ; analysis ; isolation & purification ; Neoplasms ; chemistry ; Proteome ; analysis ; isolation & purification ; Proteomics ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Objective To investigate the correlation of preoperative pain threshold and pain tolerance threshold with the intensity of stress reaction induced by endotracheal intubation and skin incision. Methods Fifty ASA Ⅰ or Ⅱ women, aged 20-55 yr, undergoing elective abdominal surgery requiring at least a 10-cm-long skin incision under general anesthesia, were studied. The electricity dolorimeter was used to measure the patients' pain sensitivity, including pain threshold and pain tolerance, and a State Trait Anxiety Inventory (STAI) was also used to examine the mental state the day before surgery. Total intravenous anesthesia was performed in all the patients.Anesthesia was induced with TCI of propofol 4 μg/ml (effect-site concentration). After patients lost consciousness,fentanyl 3 μg/kg and vecuronium 0.1 mg/kg were injected intravenously. Tracheal intubation was performed 3 min later and the patients were mechanically ventilated. MAP and HR were recorded and arterial blood samples were taken for determination of plasma concentrations of norepinephrine (NE) at 10 min after entering operation room (T1), immediately before intubation (T2), 2 min after intubation (T3), immediately before incision (T4) and 2 min after incision (T5). The differences in MAP, HR and plasma concentrations of NE before and after intubation and skin incision were calculated. SPSS 13.0 statistical software was used to analyze the correlation of STAI,pain threshold, and pain tolerance threshold with the differences in MAP, HR and NE before and after intubation and skin incision. Results Pain threshold was not correlated with the differences in MAP, HR and NE (P ＞0.05). Pain tolerance threshold was negatively correlated with the differences in MAP (r= - 0.766, r =-0.688,P＜0.05), HR (r=-0.703, r=-0.638, P ＜ 0.05) and NE (r=-0.781, r=-0.781, P＜0.05). The STAI score was not correlated with pain threshold and pain tolerance threshold (P ＞ 0.05) .Conclusion Preoperative pain tolerance threshold is negatively correlated with the intensity of stress reaction induced by endotracheal intubation and skin incision, but there is no correlation between pain threshold and the intensity of stress reaction.

Ductus Arteriosus, Patent ; therapy ; Humans ; Infant, Newborn ; Infant, Premature

ObjectiveTo investigate the effect of Xuebijing (Chinese herbal medicine preparation) on lung fibrosis in a rat model of oleic acid - induced lung injury.MethodsSixty SPF level of male Wistar rats were randomly ( random number) divided into three groups ( n =20 ):normal control group ( group A),oleic acid model group (group B),oleic acid and Xuebijing group (group C).Rats in group A were injected with normal saline (0.13 ml/kg) ; acute lung injury (ALI) model rats were injected with oleic acid (0. 13 ml/kg) intravenously in B and C groups.Twelve hours after administrations of saline or oleic acid into rats of three groups separately,samples of arterial blood from all rats were taken for blood gas analysis.By analysis of blood oxygen partial pressure,the acute lung injury model successfully made was confirmed.Then the rats in group C were treated with xuebijing (4 ml/kg) intravenously,once a day from the 1st to 7th day.The rats in groups A and B were treated with saline (4 ml/kg).Five rats in each group were randomly sacrificed on the 1st,7th,14th,28th day,separately.The bronchoalveolar lavage (BAL) of left lung of all rats was carried out after weight of lung weighed.The lefi lungs of rats were weighed in wet and dry in order to get the wet/dry ratio.Venous blood and BAL fluid (BALF) were obtained for detection of procollagen Ⅲ ( PC Ⅲ ) concentrations with enzyme - linked immunosorbent assay (ELISA).Right upper lobe of lung specimens was taken for histopathological examination after paraffin - embedded sections with hematoxylin-eosin (HE) staining and Masson staining.Multiple comparisons were analyzed by using oneway analysis of variance (SNK-q test).Statistical analyses were performed by using SPSS 13.0 software.ResultsOxygenation index of groups B and C ( PaO2/FiO2 ＜ 300) significantly decreased compared with the group A ( P ＜ 0.01 ).Levels of serum PC Ⅲ on the 14th day and 28th day in group C decreased significantly in comparison with group B ( P ＜ 0.05 ).Compared with group A,the levels of BALF PC Ⅲ and serum PC Ⅲ in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.01 ).Levels of PC Ⅲ in BALF on the 7th day,14th day and 28th day in C group decreased in comparison with group B ( P ＜0.01 ).Compared with group A,the levels of PCⅢ in BALF in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.05 ).ConclusionsThe result suggests that treatment with xuebijing may be useful in inhibiting pulmonary fibrosis during the course of ALI in rots.