Let-7 is found to be downregulated in lung cancer while many kinds of oncogenes expression always increase, and restoration of the let-7 to normal expression level inhibits lung cancer growth evidently.Thus, let-7 might serves as good targets or effective interference tools in exploration of anticancer therapeutics,and has a good prospect in applying it in the early diagnosis, prognosis and therapy in lung cancer.

Objective To study the expression of p27 gene in endometrial carcinoma and its clinical significance.Methods By using streptavidin-biotin peroxidase(SP) method the expression of p27 gene were examined in 20 normal endometrial tissues and 65 endometrial carcinoma tissues.Results p27 was expressed in 33 of 65(51%) cases of endometrial carcinomas.In contrast,p27 was all expressed in normal endometrial tissues.The expression of p27 was related to the pathological grades,clinical stages and depth of myometrial invasions.Conclusion The level of p27 expression is closely related to the growth,invasion and differentiation of endometrial carcinoma.

Objective:To investigate levels of serum IL-34 in SLE patients,and correlation between concentrations of serum IL-34 and other established serum markers and disease activity indexes.Methods: In all,78 SLE patients and 53 healthy controls were enrolled in the research.Enzyme-linked immunosorbent assay(ELISA) was employed to measuring the concentrations of serological IL-34.Then,serum IL-34 levels between SLE group and healthy controls were analyzed by Mann-Whitney U test.Meanwhile,the correlation between the serum IL-34 levels and disease activity indexes and other established serum markers were assessed.Serum IL-34 levels were significantly higher in SLE patients compared to healthy controls[(Median,128.9 pg/ml) vs (Median,52.4 pg/ml),P<0.001].Results: Their levels were remarkably associated with accumulation of the clinical features of SLE.Additionally,IL-34 titers were positively correlated with the SLE disease activity indexes,anti-double stranded DNA antibody(anti-dsDNA)titers and C-reactive protein(CRP)levels,but inversely with C3 levels.Conclusion: Serum IL-34 could be a candidate biomarker for SLE as the elevated serum levels in treatment-naive SLE patients and its association with SLE disease activity.

Objective To study the use on curative effect of Chinese medicine Qingyitang in severe acute pancreatitis.Methods 132 cases of severe acute pancreatitis were randomly divided into 2 groups,The control group(n=62) and the treatment group(n=70).Routine treatment were given in both,otherwise,the Chinese medicine Qingyitang was used in the treatment group.The operation rate,the infection rate,the time of abdominal distension removing and fasting and being hospitalized were observed and compared between two groups.Results Every index of the treatment group was finer than the control group.Conclusions The Chinese medicine Qingyitang showes their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disturbance following acute pancreatitis.As a result,the chances of bacterial translocation and enterogenic infection declines.These preparation might raise the treatment effects,as a well,shorten the course of treatment.

Mitochondria are important intracellular energy supply organelles .As semi-autonomous organelles , the mitochondrial biogenesis , damage and clearance were the dynamic processes , which are dual-regulated by mitochondrial genes and nuclear genes , and maintain mitochondrial homeostasis according to the needs of the cells for energy .Recent studies provide evidence that the disorder of mitochondrial biogenesis in the neurons participates in the pathological process after cerebral ischemia/reperfusion, resulting in metabolic disturbance and cell apoptosis .This paper reviews the research progress of mitochondrion and cerebral ischemia/reperfusion injury .

The adhesion molecules expressed by brain microvascular endothelial cell maintain the structure and function of brain, ensure the continuity of endothelium cell and penetrate blood-brain barrier in physiology. They also intervene inflammation cells that remove or orientate regular or inflammation organism in pathology. The adhesion molecules of Immunoglobulin supperfamily and Selectin family are expressed by brain microvascular endothelial cell and have affinity with the ischemic brain damage. The advance in research on relationship between the expression of adhesion molecules by brain microvascular endothelial cell and ischemic brain damage is reviewed in this article.

Qijie theory is one of the important components of TCM meridian and collateral system. It is the shortcut for the communication from the exterior to the interior and for the transverse connection, strengthens the therapeutic effects of acupuncture and moxibustion on the disorders of internal organs and enlarges the indications of acupoints. Professor LAI Xinsheng originally creats Tongyuan acupuncture technique. It is a kind of acupuncture therapeutic methods, including promoting the circulation of the governor vessel, regulating the mind and conducting qi to the origin. In order to analyze the connection between Tongyuan acupuncture technique and traditional meridian and collateral theory, the connotations of Tongyuan acupuncture technique and qijie, as well as the in-terrelationship between them are taken as the basis in the paper. By tracing the literature records, the original concept of qijie is detected. Through proving clinically Tongyuan acupuncture technique, it is revealed that qijie is the arrowhead on the main road of meridian qi circulation and is the essential connotation of the direct path of the body surface connecting with the deep layers of the body as well as the zangfu organs and tissues. It is summarized that the traditional qijie theory provides the powerful theoretic evidence for the treatment with Tongyuan acupuncture technique.
Acupuncture ; education ; history ; Acupuncture Points ; Acupuncture Therapy ; history ; methods ; History, 20th Century ; History, 21st Century ; Humans ; Qi ; history

Animals ; Electrophoresis, Gel, Two-Dimensional ; methods ; Gene Expression Profiling ; Humans ; Neoplasm Proteins ; analysis ; isolation & purification ; Neoplasms ; chemistry ; Proteome ; analysis ; isolation & purification ; Proteomics ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

This study was aimed to quickly and accurately identify different origins and categories of commodity edible bird’s nest (EBN) using DNA barcoding technique, in order to reveal its genetic differences. The total genomic DNA was isolated from the EBN samples. And Cytb gene sequences were amplified and sequenced by PCR. Then, 32 sequences were aligned and analyzed with DNAStar and MEGA 6.0 software. NJ phylogenetic tree was constructed. The nearest distance was calculated. The results showed that the original species of 32 samples of EBN were identified.Aerodramus fuciphagus was the genetic origin of 23 white nest samples. AndAerodramus fuciphagus germaniwas the genetic origin of the other 8 samples. The origin of black nest sample wasAerodramus maximusorAerodramus maximus lowi. It was concluded that the genetic origin of different EBN categories was variant. The identification of EBN’s origin species with Cytb sequence was quick and accurate.

Objective To discuss the influence of blood exchange transfusions on hemato- microenvironment of neonates with hyperbilirubinemian.Methods Blood exchange transfusion was performed in 35 neonates.Mean transfusion volume was (136.9±27.4) ml/kg.The speed of blood exchange transfusion was 92 ml/(kg·h).Blood bilirubin,blood electrolyte,blood routine and blood sugar levels were measured before,during and after the transfusion.Results Serum total bilirubin (TB) decreased by 53.78% after blood exchange,but there was no change in Na~+,Cl~-,and blood sugar (P＞0.05),and K~+ was remarkably decreased from (4.45±2.40)mmol/L to (3.87±0.52)mmol/L (t=21.979,P＜0.05).TB,Hb,PLT,WBC,Ca~(2+) were decreased[TB from(511.52±80.21) μmol/L to (236.41±66.54) μmol/L;Hb from (145.20±11.70) g/L to (128.66±2.54) g/L;PLT from (207.84±70.67)×10~9/L to (134.86±48.61)×10~9/L;WBC from (16.90±10.56)×10~9/L to (8.94±7.44)×10~9/L,and Ca~(2+) from (1.26±0.32) mmol/L to (0.99±0.22) mmol/L,P＜0.05 or ＜0.01].Conclusions Blood exchange transfusion can not influence Na~+,Cl~-,and blood sugar,but has remarkable effect on total bilirubin,Ca~(2+),Hb,K~+,PLT,and WBC.