Objective To compare the resistance of metallo-β-lactamase -producting Pseudomonas aeruginosa between burn ward and other wards, so as to provide basis for reasonable use of antimicrobial agents. Methods BD Phoenix-100 bacteria identification and drug sensitivity analyzer was used to detect Pseudomonas aeruginosa and its susceptibility, and disc synergy method was used for the screening of metallo-v-lactamase -producting Pseudomonas aeruginosa. Results Of 68 strains of Pseudomonas aeruginosa, 9 strains (13.2%) were metallo-β-lactamase (MBL) positive. Among the 9metallo-β-lactamase positive strains, 7 strains were from the burn ward and 2 strains from other wards. The resistance rate of the MBL-producing strains to ceftazidime, cefotaxime, imipenem, ciprofloxacin, amikacin, gentamicin, piperacillin and aztreonam was respectively 100.0%, 100. 0%, 88.9%, 66.7%, 88.9%, 100,0%, 100.0%, and 88.9%, obviously higher than that of non-MBL-pro-ducing Pseudomonas aeruginosa. Conclusion The rate of MBL-producing Pseudomonas aeruginosa is obviously higher in burn ward than that in other wards. The resistance of MBL-producing Pseudomonas aeruginosa is high against commonly used antimicrobial agents. Clinical use of antibiotics should he based on the antimicrobial susceptibility test results.

The landscape of HIV has changed from an incurable disease to be more like a chronic disease as the result of advances in medications; the lifespan of People Living with HIV (PLHIV) has also lengthened, HIV defining diseases are delayed, and many of the complications of HIV prevented with adherence to medications. The stigma and discrimination of HIV remain however. PLHIV need to cope with emotional issues of guilt, shame, and self-blame; social issues of rejection, termination of employment, fear and bad social experiences. The paradigm shift from negative regard of PLHIV to that of empathy, which is a deliberate and conscious attitude to relate to PLHIV as fellow travellers in life, to be able to feel with them rather than against them, has therapeutic effects. Empathy has its processes of active listening, responding with appropriately chosen words to describe feelings of the patients, and reflecting the desire to understand more about the patient’s emotions and social turmoil. The benefit of empathy in counseling are the ability to connect with the patient, to build trust from the patient being counseled, and a more objective perspective of being able to see the patient from his/her perspective rather than from the therapist’s perspective. More importantly empathy is a positive response to emotional and social issues experienced by the patient compared to platitudes like “You will be alright; or “don’t worry”, which negate and minimise the opportunity to have a catharsis.

In this paper, the research work of Cell-broken Pieces of traditional Chinese Medicine was summarized;the preparation technology, quality standard, effectiveness and safety of typical Cell-broken Pieces were introduced;the attribute, connotation and orientation of Cell-broken Pieces were discussed. It can provide reference for the fur-ther research and development of Cell-broken Pieces of traditional Chinese Medicine.

Objective To explore the curative effect of stomatitis spray combined with 3M liquid dressing on degree II red buttocks in infants and summarize key points of nursing care. Methods From April 2012 to June 2016, 64 infants with degree II red buttocks were treated in our department. According to the admission time, the infants were divided into control and experiment groups in equal number. The control group was treated with zinc oxide ointment, and the experiment group with stomatitis spray combined with 3M liquid dressing. The curative effect, total effective rate, and wound healing time were compared after treatment between the groups. Results The curative effect and total effective rate of red buttocks in the experiment group were significantly better than those of the control group (P<0.05). Moreover, the wound healing time in the experiment group was obviously shorter than that of the control (P<0.05). Conclusions Compared to zinc oxide ointment, the stomatitis spray combined with and 3M liquid dressing is more effective in the treatment of severe red buttocks of the infants. Moreover, the latter is advantageous in wound healing time, compliance, treatment, and curative effect, which indicates its potential application in clinic.

Objective To investigate the possibility of transmission of severe acute respiratory syndrome (SARS) related coronavirus by transfusion of unreliable blood and relevant product. Methods ELISA technique was used to detect the antibodies of SARS associated coronavirus in the plasma from 158 volunteer blood donors, who were medical workers, before the SARS epidemic. The subjects with positive result were place under surveillance. Results There were two positive samples in 158 tested samples. The subjects with positive test result did not have the history of SARS infection. Conclusion There was no epidemic of SARS in the hospital. The transmission pathway of SARS was unknown. It seems that the risk of transmitting SARS by unreliable blood product transfusion is just a theoretical hypothesis. With further investigation of SARS, its transmission pathway will be hopefully made known.

Objective To investigate the effect of telephone call follow-up on compliance with opening-mouth exercises among nasopharyngeal carcinoma(NPC)patients with radiotherapy-induced difficulty in opening mouth.Methods Sixty four nasopharyngeal carcinoma patients undergoing radiotherapy were randomized into control group(n=31)and observation group(n=31).The former group was given health education and instructions for functional exercises of opening mouth at discharge and regular return visits after discharge,and the observation group received regular telephone call follow-ups by an appointed nurse besides the same treatment as in the control group.The two groups were compared in regard to the compliance with the exercises of opening mouth and the incidences of difficulties in opening mouth at the first and second years after discharge. Results The compliance of the observation group was significantly higher that in the control group(P<0.01).The incidences of difficulties in opening mouth in the observation group were significantly smaller than that in those of the control at the first and second years after discharge(P<0.01). Conclusion Telephone call follow-ups are effective in the improvement of compliance of functional exercises in NPC patients with radiotherapy-induced difficulty in opening mouth,the reduction of the incidence of mouth-opening problems and the improvement of their quality of life.

A clinical emergency skill contest using emergency care simulator (ECS) was carried out　on May 2011.Total 30 family physicians in 10 teams from Shenzhen community health centers participated in　the contest.The performance and the scores of each item were analyzed.The overall score was 61.3 ± 11.9.The average score for general treatment was 33.0 ± 6.9 with a score rate of 66％ (33/50) ; the average　score for cardiopulmonary resuscitation was 23.2 ± 7.2 with a score rate of 57.5％ (23/40) ; the average　team score was lowest (5.0 ± 1.1 ) with a score rate of 50.0％ (5/10).The results indicate that family　physicians should strengthen clinical skill training of emergency care,particularly in on-site response and　teamwork.

Early diagnosis of esophageal cancer is essential for improving both the effectiveness of esophageal cancer treatment and the prognosis of patients.As a new technology for esophageal cancer early diagnosis,narrow-band imaging (NBI) enables surgeon to clearly observe the mucosa and submucosal blood vessels changes in early esophageal cancer.It has initially shown excellent application value in the early diagnosis.In particular it has obvious advantages to the ordinary white light endoscopy which is currently used in esophageal cancer early diagnosis.If combined with Lugol iodine staining,magnifying endoscopy and other diagnostic methods in clinical,NBI will have a better value in early diagnosis of esophageal.

Objective To investigate the difference in ocular surface microbiota between dry eye patients and healthy subjects,and discuss the role of microbiota in dry eye.Methods Twenty cases of dry eye patients and 90 cases of healthy subjects were collected in the PLA General Hospital and Zhongshan Ophthalmic Center.The samples of conjunctiva impression cytology were collected from all subjects,and then metagenomic shotgun sequencing was performed following the DNA extraction.The differences in alpha diversity and metabolic pathways of the ocular surface microbiota between dry eye patients and healthy subjects were evaluated.Results There was no significant difference in alpha diversity of the microbial community between dry eye patients and healthy subjects (P =0.13).However,an increase of 15 species and a decrease of 10 species were detected on the ocular surface of dry eye patients.The enriched antibiotic resistance genes in dry eye patients were more than healthy subjects.Conclusion Although the alpha diversity of the microbial community on ocular surface between dry eye patients and healthy subjects are not distinguishable,a significant difference could be found in relative abundance and metabolic pathways,suggest that these specific microbiome may be related to the pathogenesis and disease progression of dry eye.