To evaluate the diagnosis value of Lactate dehydrogenase (LDH) in meningitis, LDH activity was determined in cerebrospinal fluid (CSF) and in sera of 125 children suffering from meningitis (51 bacterial meningitis, 38 viral meningitis, and 36 tuberculosis meningitis) and 33 normal children. In blood, LDH activity elevated significantly in bacterial meningitis and viral meningitis in comparison with control group (p<0.05), but slightly in tuberculosis meningitis. However, no statistical difference between 3 groups has been found. So, LDH in blood might be not helpful in the practical diagnosis. In the CSF, LDH activity in meningitis elevated significantly in comparison with control group (p<0.001). There is highly statistically difference of activities between 3 groups. LDH activity in CSF is useful in the differential diagnosis of meningitis
Meningitis ; L-Lactate Dehydrogenase ; diagnosis

No abstract available.
Female ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Ovary*

A total of 1,132 pleural fluid culture results obtained from October 2012 to July 2014 were analyzed to elucidate the microbiological characteristics according to transudative and exudative pleural fluid. The pleural fluid cultures were performed using aerobic and anaerobic blood culture bottles. The blood and pleural fluid for total protein, lactate dehydrogenase, and glucose measurement were submitted to laboratory at the same time with pleural fluid cultures. The rates for culture positivity, anaerobes isolation, and polymicrobials between transudative and exudative pleural fluid were 5.2% vs. 10.4%, 14.8% vs. 7.8%, and 14.8% vs. 10.9%.
Exudates and Transudates ; Glucose ; L-Lactate Dehydrogenase

No abstract available.
Hematologic Neoplasms* ; L-Lactate Dehydrogenase* ; Lactic Acid*

BACKGORUND: Trauma, surgical stress, and anesthesia are often associated with postoperative immune suppression and an increased susceptibility to infection. The role of propofol in a patient who may be at the risk of impaired immune function is contradictory. To access the possible role of propofol on human immune function, we investigated the cytotoxic activity of mononuclear cells from peripheral blood. METHODS: Healthy human mononuclear cells (MNCs) were isolated and stimulated with lipopolysaccharide (LPS) for 5 hrs. Activated MNCs were cultured in the presence of varying concentrations of propofol for 20 hrs and lactate dehydrogenase (LDH) release was measured to evaluate NMC cytotoxicity against K-562 cell target cells (cell to target 40:1). RESULTS: Propofol exposure at concentrations of 1, 5 and 10mug/ml did not significantly affect LDH release from K-562 cells, but the cytotoxic activity of MNCs was significantly suppressed at a concentration of 50mug/ml. (P<0.01) CONCLUSiONS: Since the concentrations of 1, 5 and 10mug/ml of propofol are in the clinically acceptable range for sedation and anesthesia, this result suggest that propofol does not significantly alter the cytotoxicity of NMCs in septic conditions.
Anesthesia ; Humans ; L-Lactate Dehydrogenase ; Propofol*

The purpose of the present study was to investigate the effects of resveratrol supplementation on oxidative damage and lipid peroxidation induced by strenuous exercise in rats. The rats were randomly divided into five groups: a sedentary control group, an exercise control group, and three treatment exercise groups administered increasing doses of resveratrol (25, 50, and 100 mg/kg body weight). Resveratrol was administered by oral gavage once daily for four weeks. At the end of the four-week period, the rats performed a strenuous exercise on the treadmill, and the levels of lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), and 8-hydroxy-2\'-deoxyguanosine (8-OHdG) were measured. The results showed that resveratrol supplementation had protective effects against strenuous exercise-induced oxidative damage and lipid peroxidation by lowering the levels of LDH, CK, MDA, 4-HNE, and 8-OHdG in the serum or muscle of rats. These beneficial effects are probably owing to the inherent antioxidant activities of resveratrol.
Animals ; Creatine Kinase ; L-Lactate Dehydrogenase ; Lipid Peroxidation* ; Malondialdehyde ; Rats*

OBJECTIVE: Creatine phosphokinase-MM(CPK-MM) and lactate dehydrogenase(LDH) are well known indices of muscular injury. To know the degree of muscular injury during spinal surgery, the author report serial measurement of serum CPK-MM and LDH4 level. METHODS: The authors investigated 23 patients who underwent lumbar spinal surgery without bone fusion or instrumentation. Peripheral venous blood samples were serially collected 1 day before surgery, operation day, postoperative 3rd, 7th, 14th and 28th day. Postoperative back pain was measured by visual analogue scale. We evaluated the relationship between number of operation level, serial changes of serum CPK-MM, LDH4, duration of surgery and postoperative back pain. RESULTS: CPK-MM activity was higher after surgery than before it and reached at maximal level on the postoperative 3rd day, and it was returned to normal level on the postoperative 7th day. The score of postoperative back pain scale was the highest on the postoperative 3rd day. CPK-MM activity was significantly correlated with operation level, duration of surgery, and postoperative back pain(P<0.05). In contrast, serum level of LDH4 showed no significant correlation with duration of surgery(P>0.05). CONCLUSION: Postoperative muscle injury is inevitable in all patient who underwent spinal surgery, and these injuries are related extent of exposure and duration of surgery. To reduce muscle injury and postoperative back pain, less invasive and shorter surgery in time is recommend.
Back Pain ; Creatine ; Humans ; L-Lactate Dehydrogenase ; Lactic Acid ; Spine*

OBJECTIVE: To investigate the prognostic value of the serum calcium level corrected by serum albumin (cCA) and corrected serum lactate dehydrogenase (LDH) level for the risk stratification for newly diagnosed multiple myeloma (MM) patients. METHODS: The clinical data and survival of 186 newly diagnosed MM patients admitted to our hospital from June 1, 2015 to November 1, 2017 were collected. The patients's survival time was obtained by telephone and follow-up visits to patients and their families. The value of the prognostic system consisting of cCA levels and LDH levels in the survival time of MM patients was retrospectively analyzed. Moreover, the post-corrected hypercalcemia and high LDH as 2 factors were used for risk stratification, then according to these 2 factors, the MM patients were divided into 3 groups: group 1 (no risk factor), group 2 (1 risk factor) and group 3 (2 risk factors), and the effect of risk factors on the prognosis of MM patients was analyzed. RESULTS: The median follow-up time was 16 months. The cumulative OS rate of the post-corrected hypercalcemia group was lower than that of the non-hypercalcemia group. The 1-year cumulative OS rate in the 2 groups was 79.0%±6.7% and 88.6%±3.0%, the 3-year cumulative OS rate was 53.0%±10.5% and 74.6%±6.6% (P=0.016), respectively. The cumulative OS rate of the high LDH group [LDH ＞upper limit of normal (ULN), ULN=250 U/L] was lower than that in the normal LDH group. The 1-year cumulative OS rate in the 2 groups was 71.6%±8.6% and 90.0%±2.8%, the 2-year cumulative OS rate was 44.9%±12.1% and 83.1%±4.0%, respectively, and the median OS time was 19 months (95%CI: 15.32-23.34) and not reached (P=0.001). The risk stratification analysis showed that the median OS time of the 3 group was not reached (n=103, 57%), not reached (n=70, 39%) and 17 months (n=7, 4%, 95%CI: 5.19-28.41, P＜0.001). Patients with two risk factors had a prognosis worse than patients with 0-1 risk factor. CONCLUSION The prognostic combination of corrected serum calcium and LDH levels may provide a basis for risk stratification and prognosis in MM patients in clinical practice.
Calcium ; Humans ; L-Lactate Dehydrogenase ; Multiple Myeloma ; Prognosis ; Retrospective Studies

OBJECTIVETo explore the adaptive mechanism to hypoxia in skeletal muscle of tibetan antelope.

METHODSTibetan sheep which living at the same altitude (4 300 m) with tibetan antelope and low altitude (1 800 m) sheep as control, the content of myoglobin (Mb) and lactic acid (LA), the activity of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in skeletal muscles among three animals were analyzed by spectrophotometer.

RESULTSThe content of myoglobin in skeletal muscle of tibetan antelope significantly higher than that of tibetan sheep and low altitude sheep (P < 0.05). And the content of LA in skeletal muscle of tibetan antelope significantly lower than that of tibetan sheep and low altitude sheep (P < 0.05), activity of LDH and MDH in skeletal muscle was significantly lower and higher respectively than that of tibetan sheep and low altitude sheep (P < 0.05). There was no significant difference between tibetan sheep and low altitude sheep.

CONCLUSIONTibetan antelope may improve their ability to get oxygen under hypoxia by increasing the content of myoglobin in skeletal muscle, and the proportion of aerobic metabolism is high in skeletal muscle, it may be relate that with high myoglobin content in skeletal muscle, we suppose that high myoglobin content in skeletal muscle of tibetan antelope might be one of the molecular basis to adapt hypoxia.

Altitude ; Animals ; Antelopes ; metabolism ; physiology ; Hypoxia ; metabolism ; L-Lactate Dehydrogenase ; metabolism ; Malate Dehydrogenase ; metabolism ; Muscle, Skeletal ; metabolism ; Myoglobin ; metabolism

OBJECTIVE: To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury. METHODS: Forty New Zealand rabbits were randomly distributed into control group and electrical injury group (EI-groups; designated 7 time points: 0 h, 2 h, 4 h, 12 h, 1 d, 2 d, 3 d), 5 rabbits per each group. EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory. Ventricular blood (5 mL) was obtained under anesthesia at designated time points after electrical injury. The activities of LDH and HBDH were measured. RESULTS: Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury. Compared with control group, the activities of LDH increased markedly at 4 h, 12 h, and on days 1, 2, and 3 after injury (4 h, 12 h, and day 1 P<0.01; day 2 and day 3 P<0.05). Activities of HBDH increased markedly at day 2 and day 3 after injury (P<0.05). The ratio of HBDH/LDH decreased markedly at 2 h, 4 h, and 12 h after injury (P<0.01). CONCLUSION Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.
Animals ; Electric Injuries/enzymology* ; Female ; Forensic Pathology ; Hydroxybutyrate Dehydrogenase/blood* ; L-Lactate Dehydrogenase/blood* ; Male ; Rabbits ; Random Allocation