Objective:To investigate the expression and distribution of aquaporin 5(AQP5) in allergic rhinitis(AR)treated by fluticasone propionate and levocabastine.Method:Forty Wister rats were divided randomly into AR(n=30) and control groups(n=10). After AR models were established, the AR rats were divided evenly into F group, L group and AR control group.Three groups were treated respectively for 28 days, then the expression of AQP5 in nasal mucous membrane were detected by immumohistochemistry assay.Result:The distribution of AQP5 was consistent in all groups. The expression of AQP5 in F group was significantly different from L group and AR group (P < 0.05), while there was no significant difference between that of AR group and L group(P>0.05). The expression of AQP5 in L group was significantly different from that in control group(P<0.05).Conclusion:High expressions of AQP5 in rat with AR indicated the positive correlation between AQP5 and AR. AQP5 might be one of pathological factors of AR concered with gland's excessive secretion and tissue edema. Glucocorticoid can down-regulate the expression of AQP5,but H1-receptor antagonist can not reduce the expression of AQP5.

Objective To explore the composition situation of the risk factors of patients with stroke in Huainan area. Methods 471 continuous registration and hospitalized patients with stroke in Huainan were selected,of which,362 cases were ischemic stroke,109 cases were hemorrhagic stroke,and the known risk factors were statistically analyzed. Results Of all patients,the incidence of hypertension was first(69. 3% was in ischemic stroke, 77. 9% was in hemorrhagic stroke) ,and hypertension was the most important risk factors. The composition of risk factors in stroke patients of different gender were different,and the smoking,drinking problem was more prominent in male patients (51.1% ,33.2%respectively)than that in female patients(5.1% ,2.0% respectively) (x2 =111-81,67.62, all P＜0.01) ;The incidence of diabetes, heart disease, atrial fibrillation was higher in female patients (37. 6% ,31. 9% ,19.3%respectively) than that in male patients (22.3% ,20.4% ,8.8% respectively) (x2 = 13. 12,8.09,11. 12,all P＜0.01). Hypertension,diabetes accounted for only 40.8% and 47.4% of patients taking regular medication. Atrial fibrillation was more common in patients with hemorrhagic stroke. Conclusion Controlling blood pressure and blood sugar was still important in the primary prevention and secondary prevention of stroke in the region. People of different gender should be targeted for individual health education, and promoting a healthy lifestyle should be paid attention, so as to the occurrence of stroke could be effectively prevented.

Acupuncture Therapy ; Edema ; etiology ; immunology ; therapy ; Female ; Humans ; Lower Extremity ; pathology ; Middle Aged ; Ovarian Neoplasms ; surgery ; Postoperative Complications ; etiology ; therapy

Objective: To study the influence of different telomere oligonucleotide on rat's thoracic aortic smooth muscle (A7R5 ) cell apoptosis and to clarify weather silent information regulator 1 (SIRT1)/cancer suppressor gene (P53) signaling pathway involved in oligonucleotide induced cell apoptosis. Methods: 3 telomere repeat sequences of TE-12 (TTAGGG)2, TE-24 (TTAGGG)4 and TE-36 (TTAGGG)6 were respectively transfected into rat's A7R5 cells as TE-12 group, TE-24 group and TE-36 group; in addition, blank A7R5 cells were used as Control group. Transfection induced A7R5 cell apoptosis was detected by flow cytometry, mRNA and protein expressions of SIRT1 and P53 in A7R5 cells were examined by RT-PCR and Western blot analysis in different groups. Results: Successful ransfection rates were similar among 3 groups. Compared with Control group and TE-36, TE-24 groups, the highest apoptosis rate of A7R5 cells was found in TE-12 group and the lowest was found in TE-24 group which was still higher than that in Control group, allP<0.05. mRNA and protein expressions of SIRT1 was obviously higher in TE-12 group than the other 3 groups, allP<0.05; mRNA and protein expressions of P53 was obviously lower in TE-24 group than the other 3 groups, allP<0.05. Conclusion: Telomere oligonucleotide sequence may promote rat's A7R5 cell apoptosis; different sequence had various influence and the strongest effect was observed in TE-12 sequence. The above impact might be related to SIRT1/P53 signaling pathway.

Objective To investigate the possibility that TGF ? may also influence the basal and FSH stimulated production of estradiol and progesterone by granulosa cells in vitro. Methods Granulosa cells were obtained from infertile patients undergoing in vitro fertilization and embryo transfer treatment and cultured with serum free supplemented HAM′s F 10 medium(control) or increasing concentrations of TGF ? 1,2 in the absence or presence of FSH (96 U/ml). The media were collected in 24, 48, 72 and 96 h intervals and assayed for estradiol and progesterone. Results Increasing amounts of TGF ? had a significant effect on estradiol production, especially in the presence of FSH,The estradiol value was evaluated from (12 077.9?5 835.3) pmol/L and (13 656.1? 8 404 3) pmol/L to (16 606.8?7 993.3) pmol/L and (19 480.4?913.8) pmol/L. TGF ? inhibited FSH stimulated progesterone but not unstimulated progesterone release ( P

Objective To understand the pollution status of indoor volatile organic compounds(VOCs) and to provide evidence for preventing the adverse effects of them on resident health.Methods According to the different times of the house decoration, 53 residents were randomly selected from a district of Dalian.Air was sampled in the bedrooms, the kitchens and the outdoors for 24 h by sampling tubes which were activated by activated carbon and the concentrations of VOCs were analyzed by GC-MS/ SIM.Results The concentrations of VOCs indoor were higher at the half year after decoration.The concentrations of methyl isobuthyl ketone, butyl acetate, 1, 2-dichloroethane, 1, 1, 1-trichlo-roethane, tetrachlorocarbon, hexane and buthanol were 0.60, 9.15, 3.10, 1.00, 1.67, 1.90 and 14.50 ?g/m3 in the bedrooms;they were 0.60, 8.10, 3.95, 1.74, 0.85, 1.87 and 12.45 ?g/m3 in the kitchens.The concentrations of VOCs indoor were still higher than those of outside atmosphere(P

Objective We aimed to study the optimal method for local compression following removal of needle after intravenous injection and protect patients' vein to the highest degree. Methods 620 patients were divided into the observation group and the control group with 310 cases in each group randomly. Comparison study was carried out by adopting interchange compression (routine method) and noninterchange compression (direct pressing with hypothenar). Results The incidence of pain, bleeding and subcutaneous stasis after removal of needle in the observation group was lower than those in the control group (P<0.01). Conclusions Local compression following removal of needle with hypothenar was superior to routine compression method and it was worth applying in clinics widely.

Objectives To compare the effects of different early enteral nutrition formula on improving metabolic status of burned patients. Methods Thirty cases of burned patients with total burn surface area (TBSA) more than 30% were randomly divided into two groups:⑴Group F, enteral feeding with Fresubin 750MCT containing MCT/LCT as fat source, ⑵Group N, enteral feeding with Nutrison containing only LCT as fat source. All of the thirty patients received isocaloric, isonitrogenous and isolipidic enteral feeding with intragstric tube and enteral feeding pump, within 24h of postburn. Plasma insulin, glucagons, tumor necrosis factor (TNF) and 3-methylhistidine in urine were measured on day 1,4,7and 10 days of postburn. Results The results showed that the patients of group F had an increased blood concentration of insulin and the ratio of insulin/ glucagons, a decreased plasma concentration of TNF, and a reduced excretion of 3-methylhistidine in urine as compared with the patients of group N. Conclusions An enteral compound diet containing MCT/LCT in the early enteral feeding in burned patients can improve the metabolic status of burned patients more effectively.

Objective To definite the correlation between allergic diseases with the occurrence risk and prognosis of colorec tal cancer by meta analysis.Methods The databases of PubMed,MEDLINE,EMBASE,Web of Science,CNKI and Wanfang data were retrieved by computer.The time limit was from January 1988 to October 2015.The literatures were screened and the data were extracted according to the inclusion and exclusion criteria.The meta analysis was performed by using STATA12.0 software.Results A total of 16 articles(ll cohort studies,5 case-control studies)were included in this study.The meta analysis showed that the risk of colorectal cancer occurrence in the patients with allergic diseases was significantly lower than that of the control group(OR=0.88,95％CI:0.80-0.98,P＜0.05;the stratified analysis found that the risk of colorectal cancer occurrence in the patients with hay fever(OR=0.93,95％CI..0.87-0.99,P＜0.05)and patients with allergic dermatitis(OR=0.73,95％CI:0.56-0.95,P＜ 0.05) were lower than that of the control group.Compared with the control group,the death risk of colorectal cancer in the patients with allergic diseases was significantly lower than that of the control group(OR=0.73,95 ％ CI:0.85-0.97,P＜0.05).Conclusion The patients with allergic diseases may reduce the occurrence risk and death risk of colorectal cancer through enhancing the recognition ability of immune system and destroying tumor cells.

Objective To review 2006-2007 AIDS response in China by applying the indicators of "Monitoring & Evaluation Plan of AIDS Response". Method Data from the national AIDS routine reporting system and special survey in line with the definitions of the indicators and standardized methods were summarized,classified and analyzed; mid-term targets or indicator value of 2005 were employed as comparison. Results The 2007 result of the national HIV sentinel surveillance showed that the HIV prevalence among female sex workers,injecting drug users,men who have sex with men and pregnant women was 0.0%,1.9%,3.1% and 0.0% respectively;the data from the national HIV comprehensive response information system showed that by the end of 2007,72.8% of the adults and children with advanced HIV infection had received antiretroviral therapy (ART) ,and 83.6% of the ART receivers survived after 12 month treatment. Conclusion Indicators of the "Monitoring & Evaluation Plan" are able to reflect the outcomes and achievements of 2006-2007 HIV/AIDS response in China,which has made good progress and has fulfilled the mid-term targets as defined by the Plan.