Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy,TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells,and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.

Aged ; Female ; Hemangioma, Cavernous ; complications ; Humans ; Kasabach-Merritt Syndrome ; complications ; Liver Neoplasms ; complications

Objective:To enhancement recognition of the toxic effect of benzene on hematopoietic tissue and karyotypic abnormalities. Methods:To report 1 case of benzene poisoning patient diagnosis in morphological features of bone marrow cells and abnormal karyotype and to review literature. Results:The morphological features of bone marrow cells was multisystem morbid hematopoiesis to diagnose myelodysplastic syndrome and the chromosomes submit complicated abnormal karyotype. Conclusion:Benzolism can cause the myelodysplastic syndrome and the chromosomes alter diversify. It acts important effect on morbility of the myelodysplastic syndrome.

Objective:To observe the therapeutic effects and side effects of FAT (fludarabine,cytarabin,and topotecan)regimen for relapsed or refractory acute leukemia.Methods:The regiment consisted of Flu 25 mg/ (m2?d)iv 30 min,from dl to d5;Ara-C 1.0 or 2 g/ (m2?d)iv 4 h,from dl to d5,and Top 1.2 mg/(m2?d)iv 2h,form d1 to d3. G-CSF was to employ from WBC 4?109/L .9 cases of refractory or relapsed acute leukemia were treated with this regimen.Results:In all the 9 cases of leukemia,6 cases achieved the complete remission (6/9),1 case had achieved partly remission and 2 cases had no response .The main toxicities were myelosupression,fever,diarrhea and slight hepatic injury .Conclusion:FAT regiment shows favorable outcome for refractory or relapsed acute leukemia,and side effects can be tolerance .It can be employed for the patients with refractory or relapsed acute leukemia who had no response to the first line regimen or other salvage regimens and get a chance for further hematopoietic stem cell transplantation.

Acute Disease ; Adolescent ; Adult ; Aged ; Female ; Herbicides ; poisoning ; Humans ; Male ; Middle Aged ; Paraquat ; poisoning ; Poisoning ; drug therapy ; Young Adult

Objective To analyze the distribution characteristics and antimicrobial resistance of isolated Acinetobacter(A.)bau-mannii from the clinical inpatients in our hospital from 2008 to 2013 to provide the basis for rational use of antibacterial drugs in clinic.Methods The BD phoenix-100 fully automatic microbial analyzer(USA)was adopted to conduct the strain identification and the minimal inhibitory concentration(MIC)detection of clinically isolated A.baumannii.All obtained data were analyzed.Results 128,173,350,282,186 strains of A.baumannii in turn were isolated during these years,the strains in sampling from the clinically i-solated A.baumannii during 2012 was mainly originated from the sputum samples(155 strains,68.28%).In the distribution of hos-pital departments,most concentrated in ICU(65 strains,28.63%),followed by the respiratory department(47 strains,20.70%),the emergency observation wards(35 strains,15.42%)and the apoplexy and geriatric wards(24 strains,10.57%),the most of patients were older people aged more than 60 years.And according to the data within these 5 years,many kinds of different antibacterial drugs had generally the increased drug resistance rate,especially the increasing trend during 2011-2012 was more obvious,the pen-icillins all showed extensive drug-resistance.In the third,fourth generation cephalosporins,aminoglycosides and quinolones were generally drug-resistant.The drug resistance rate of cephalosporins(ceftazidime,cefotaxime,cefepime)remained at a higher level of 75.00%-85.00%.The drug resistance rate of quinolones(ciprofloxacin,levofloxacin)and aminoglycosides(gentamicin,amikacin) also showed the increasing trend year after year.The drug-resistant rate of the beta lactam compound preparations had the rapidly increasing speed,such as which of ampicillin /sulbactam was increased from 60.16% to 83.33%,which of carbapenems such as imipenem and meropenem was increased more significantly,the drug-resistant rate of imipenem was increased from 25.20% to 82.27%,and the detection rate was continually increased.And the drug resistant rate of Schupson was increased from 18.89% to 80%,but the only difference was the drug named Colistin(polymyxin E)maintained a low drug resistance rate during these five years.Conclusion Resistance of A.baumannii to various antibacterial drugs is gradually increased and the isolation rate of multi-drug-resistant and pan-drug resistant A.baumannii is increased year by year.So A.baumannii infection and its drug-resistance changes should be vigilant and be paid more attention to for preventing the emergence and spread of drug-resistant A.baumannii strains.

This study was to evaluate the clinical application of thinner ceramic veneer restoration. Tooth color was measured by eye and colorimeter after thinner ceramic veneer restoration. The results showed that there were many factors influencing the color of its clinical restoration. Hue of the ceramic veneer, ceramic materials and bond composites had greater influence than that of dentition. The brightness of ceramic materials and bond composites was closely correlated with the whole restoration brightness. A closer correlation was found with the brightness of bond composite. Thinner ceramic veneer restoration can achive satisfactory results.

With the change of clinical infection patterns,increasing number of multi-drug resistance bacteria and emerging of new discovering pathogens,the knowledge of Clinical Microbiology has developed and updated rapidly.It will produce positive effect on students'competence cultivation to introduce the knowledge about new techniques application and related subjects in student's clinical teaching practice.

Objective: To explore the efficacy and complications of allogeneic peripheral blood stem cell transplantation (Allo-PBSCT) followed by sequential infusion of donor-derived cytokine induced killer (CIK) cells for relapsed/refractory hematologic malignancy. Methods: Nine patients with relapsed/ refractory hematologic malignancy without complete remission received Allo-PBSCT. After hematopoietic reconstitution, the donor-derived peripheral blood mononuclear cells were collected by blood cell separator and cultured into CIK cells in vitro . The CIK cells were infused back into the patients. The therapeutic efficacy, adverse reactions and prognosis were observed. Results: All patients engrafted. Complete remission was also achieved in all patients. After infusion of CIK cells, four patients developed graft-versus -host disease (GVHD) which was relieved by reinforced immunosuppression therapy. One patient died of infection 59 days after Allo-PBSCT, and the other 8 patients survived at a median followup of 9.2 months (2-20 months). Conclusion: Allo-PBSCT followed by sequential infusion of donor-derived CIK cells can salvage the patients with relapsed/refractory hematologic malignancy and it is worthy of further large-scale study. Copyright © 2012 by TUMOR.

AIM: To study the expressions and significance of stem cell factor(SCF) and tyrosine kinase receptor(KIT) in neurons after cerebral ischemia reperfusion injury.METHODS: Western blotting was used to examine the expression of SCF and KIT in the cortex after cerebral ischemia and oxygen glucose deprivation(OGD) neurons,then the OGD neurons preincubated with SCF and SCF-Ab were assessed the cell viability by MTT to determine its significance.RESULTS: ①Compared to control group,the expressions of M-SCF and S-SCF in cortex increased markedly while KIT decreased significantly in ipsilateral hemisphere.However,no obvious change in the contraleteral hemisphere was observed.②Compared to control group,the levels of S-SCF and M-SCF were increased significantly in the OGD neurons while KIT was not obvious changed.③The cell viability of OGD neurons decreased significantly.Preincubation with SCF inhibited cell damage while SCF-Ab deteriorated OGD injury.CONCLUSION: The expression of SCF may be induced by cerebral ischemia reperfusion,which can protect the neurons from ischemia injury.