Epidemiological and pathological evidence has demonstrated that adverse in utero environment,such as protein restriction,hypercholesterolemia,diabetes and smoking,can increase the risk of vascular disease in the offspring.In utero,the placenta and fetus are exposed to the metabolic,antioxidant and pro-inflammatory and anti-inflammatory signals from the mother and likely to make specific responses,which may lead to permanent changes either in DNA methylation or chromatin modification or both,and these changes,in turn,may result in increased atherosclerosis susceptibility in adulthood.In this review,we briefly summarize the possible signals crossing the placental barrier and discuss the molecular mechanisms of epigenetic programming in the developing fetus that may lead to increased athero-susceptibility of the vessel wall.

To collect the literatures which are the treatments of sjogren's syndrome with pulmonary fibrosis by traditional Chinese medicine. It is including the etiology and pathogenesis, specific prescription and medication, clinical proved recipe, single herb and experimental research to summarize the traditional Chinese medicine treatment of sjogren's syndrome with pulmonary interstitial fibrosis. After reviewing Literatures, the Chinese medicine to the sjogren's syndrome with pulmonary fibrosis has a vast developing prospect and deserves further study.

Objective To set up an information platform applicable to medical non-commissioned officers (NCOs) so as to integrate,multiply and share teaching resources,and optimize and improve training abilities.Methods The downstage application and backstage management were realized by ASP and WEB database program,as well as by dynamic renewal of web pages via backstage adjustment.Results Based on local area network,an information platform applicable to medical NCOs' education was set up,on which the goals,including digitization of textbooks,integration of courseware,standardization of picture and word materials,automatic formation of teaching plans,and upload,download and broadcast of courses,were reached.Conclusion The educative digitization can be realized for medical NCOs by means of computerized network,database and multimedia techniques,which helps to push forward the innovation of educative modes and approaches.

Objective To report the experience of the revision procedure with morselized bone grafting and a special design of acetabular prosthesis with three wings for loosened acetabular component in our hospital. Methods Since December 1989 to Junanry 1999, 23 cases with loosened acetabular component and bone defect were revised by morselized bone auto/allografting. There were 12 males and 11 females, the average age of the patients was 54.2 years old (range from 38 to 71 years old). The prosthesis of primary THA was double cup in 4 cases, the conventional THA in 19 cases, cemented in 22 cases and cementless in 1 cases. The severity of acetabular component loosening was classified into four types according to Gustillo: Type II in 3 cases, type III in 9 cases and type IV in 11 cases. The morselized bone autografting and allografting with size 4－ 6 mm were used in 6 cases and in 17 cases respectively. Different acetabular components had been used, the thread metal acetabular cup in 2 cases, screw fixed conventional metal acetabular cup in 10 cases and special design acetabular prosthesis with three wings in 11 cases. The latter prosthesis consists of an conventional acetabular component with pearl porous surface and three wings with different length and sharp edge distributed centrally in the superior lateral quadrant on the surface, the liner is made of UHMPE. Results No infection of the wound occurred in this group. The mean time of follow- up was 3 years and 8 months. Except 3 cases, all of the bone grafts was healed well and X－ ray showed that the containing of acetabular component was good, no translucent line between donor bone bed and prosthesis, and the bone defect had been repaired. The average Harris score was 47 before revision, and 86 after revision. The successful rate of revision was 86.9％ . There were 3 loosening cases postoperatively, 2 cases were due to incorrect position of prostheses and insufficient bone graft, another case was due to the screw being too short to fix the acetabular component. However, all of the three cases have partial bone growth in the original loosening acetabular. Conclusion 1. Direct contact between the morselized bone auto/allograft and the surface of metal acetabular component and the firm fixation of the prosthesis have showed good result of bone defect reconstruction. 2. The special design of acetabular prosthesis with three wings could be selected as an alternative method for huge bone loss of acetabulum (ranged from 64 to 79 mm), because it was able to enlarge the volume of the acetabular component.

Objective To explore the practical method and teaching effect of problem-based learning (PBL) pattern in teaching of gastrointestinal disease for seven-year-program students during their internal medical probation.Methods The traditional pattern (gastrointestinal diseases) and PBL pattern (hepatobiliary diseases) were applied in these students to assess the teaching effects by exam scores and questionnaire.Results ① There was no significant difference between traditional training and PBL by exam scores (P＞0.05).② The PBL approach was widely accepted by students,which could contribute to the improvement of their learning motivation.It developed skills,including self-directed learning,summarizing,and clinical thinking capability and so on.Conclusion The PBL approach is a practical learning strategy that can improve the teaching effect; therefore it should be wildly practiced in future works.

This study aimed at exploring the modeling and identification method of autoimmune POF by the ZP3 polypeptide with the provision of scientific basis for the prevention and treatment of POF.B6AF1 female mice,the hybridization of A/J male mice and C57BL/6 female mice,was immunized by zona pellucida polypeptide fragments.Then,the mice estrous cycle was detected by vaginal exfoliated cells Pasteur's staining.After the 14-day modeling,the mice model was identified on the 7th and 14th day,respectively.Ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change were detected by H&E staining and ZP3 immunofluorescence staining.Serum hormone levels of E2 and FSH in the mice were tested by ELISA,with the combination of estrous cycle,ovarian tissue morphology and anti-zona pellucida antibodies immunofluorescence change to judge the success of the POF mice model.It was found that ZP3 polypeptide immunized mice appeared the disorder of estrous cycle after 14 days,so did the marked inflammation observed by ovarian biopsy,a clear transparent zone by ZP3 immunofluorescence staining,and the decrease of serum E2 declined and the increase of serum FSH by ELISA.However,no significant change was found on the 7th day in the modeling.In conclusion,ZP3 polypeptide induced autoimmune POF mice model was established successfully,featuring high incidence and simple method and laying a foundation for the further study of autoimmune POF.

Objective:To observe effects of Tongmai Yizhi Capsules on trace elements in the patient of vascular dementia.Methods:Blood trace elements Cu,Se,Zn and Fe contents before and after treatment were investigated in a Tongmai Yizhi Capsules group(n= 50)and a Hydergine group(n=30).Results:After treatment Cu,Se contents increased and Zn and Fe contents decreased in Tongmai Yizhi Capsules group with significant difference as compared with Hydergine group(P

The health inspection and quarantine specialty is a new undergraduate specialty published by Ministry of Education in 2012.But the health inspection professional or preventive medicine (health inspection subspectialty) set in our country put more emphasis on the content of health inspectionor medical examination,rarely involved in the Quarantine content.Therefore,according to the new professional requirements and training objectives,combined with other quarantine specialty course teaching experience and the disciplinary characteristics,we explored a feasible way to develop the discipline and training,from the health and quarantine courses setting,teaching theory,teaching practice,teaching evaluation and management aspects.

Objective To evaluate the effects of leg ischemic postconditioning on focal cerebral ischemia-reperfusion (I/R) injury in diabetic rats. Methods Forty male diabetic SD rats weighing 270-320 g were randomly assigned to one of 4 groups (n=10 each):group Ⅰcontrol (C);group Ⅱ sham operation (S);group Ⅲ fecal cerebral ischemia-repertusion (I/R) and group Ⅳ leg ischemic postconditioning (IPC). Diabetes mellitus was induced by intraperitoneal streptozotocin. Local cerebral I/R was produced by middle cerebral artery occlusion (MCAO) for 1.5 h followed by 6 h reperfusion. A nylon thread with rounded tip was inserted into internal carotid artery and threaded cranially until resistance was felt. Leg isebemia was induced by placing tourniquet on beth legs at 30 min before repeffusion. The animals underwent 3 episodes of 5 min leg ischemia at 5 min intervals. Neuro-functional deficit was assessed and recorded at the end of 6h reperfusion using neurologic deficit scores (NDS) (0=no deficit, 4=unable to crawl and semiconscious). The animals were then decapitated and their brains were removed for determination of volume of cerebral infaret (by TIC staining) and ratio' of neuronal apoptosis (by TUNEL). Results NDS and cerebral infarct volume were significantly increased at the end of 6 h reperfusion in group I/R and IPC as compared with group C and S. IPC significantly decreased cerebral infarct volume and ratio of neuronal apoptosis induced by MCAO. There was no significant difference in NDA between group I/R and IPC. Conclusion Leg iscbemic postconditioning can protect the brain against focal cerebral I/R injury in diabetic rats.

To study the peptidergic innervation in cardiac adenocarcinoma, immunohistochemistry method was employed to examine the distribution of ten kinds of neuropeptide immuno reactive nerve fibers in human cardiac adenocarcinoma, which was surgically obtained, and their relationship with tumor cells. Substantial amount of many kinds of neuropeptides including GAL, Dyn, NPY immuno reactive nerve fibers were observed in the tumor. Most of them were closely in contact with tumor cells. It is possible that there is peptidergic innervation in cardiac adenocarcinoma.