This paper introduces such information of database server platform for military ″1″ hospital information system as its history, present status and progress. The IA-64 calculation platform based on open standard and Oracle 9i are also summarized. Upgrade experiment and evaluation are carried out, which all lay the foundation for the following upgrade of the server platform and can be referred to on relative functions.

Objective: To make it clear whether there exists m elatonin receptor in the thyroid of human embryo. Methods: Thyr oid was collected and sliced up to be stained with methods of immunohistochemis try and in situ hybridization. Results: The thyroid tissue w as p ositively dyed, melatonin receptor mt1 and MT2 were with both immunohistoche mistry and in situ hybridization while brown granules dep osited in the membrane, plasma and nuclear of the thyroid cell were with the imm unohitochemistry. Conclusion: There exists melatonin rece ptors in human embryo thyroid, either mt1 or MT2, and they exist in the memb rane, plasma and nuclear.

Humans ; Pneumoconiosis ; therapy ; Self Care

Objective: To make it clear whether there exists melatonin receptor in the thyroid of human embryo. Methods: Thyroid was collected and sliced up to be stained with methods of immunohistochemistry and in situ hybridization. Results: The thyroid tissue was positively dyed, melatonin receptor mt 1 and MT 2 were with both immunohistochemistry and in situ hybridization while brown granules deposited in the membrane, plasma and nuclear of the thyroid cell were with the immunohitochemistry. Conclusion: There exists melatonin receptors in human embryo thyroid, either mt 1 or MT 2, and they exist in the membrane, plasma and nuclear.

We review the proceedings on the methods of how the aromatase inhibitor maintains its function, the recently developed drugs and the progress in the field of endocrine therapy for human breast cancer

Aim To investigate the anti-proliferative effect of salinomycin on doxorubicin-resistant human breast cancer MCF-7 /DOX cells.Methods MCF-7 and MCF-7 /DOX cells were treated or untreated with salinomycin.Cell viability was detected by MTS assay. Cell apoptosis was detected by Annexin V-FITC /PI as-say.Reactive oxygen species (ROS)was measured by DCFH-DA staining.Mitochondrial membrane potential was measured by JC-1 assay.The expression of apopto-sis related proteins BAX, BCL-2, caspase-3, and caspase-9 were evaluated by Western blot analysis. Results The cell viability was significantly reduced by salinomycin treatment in a dose-dependent manner. The flow cytometry results showed that salinomycin in-duced MCF-7 /DOX cell apoptosis,increased ROS pro-duction,and decreased mitochondrial membrane poten-tial.Furthermore,salinomycin decreased the expres-sion of BCL-2,and increased the expression of BAX, cleaved caspase-3,and cleaved caspase-9.Moreover, the antioxidant N-acetylcysteine (NAC ) markedly blocked the above effects.Conclusions Our results suggest that salinomycin-induced apoptosis in MCF-7 /DOX is associated with induction of ROS production, and activation of mitochondria apoptosis pathway, which may become a potential chemotherapeutic agent for the therapy of doxorubicin resistant breast cancer.

This article unscrambled the concept of pathogenesis theory,through textual research of literatures,directly explained the concept connotation.From the relationship between pathogenesis and symptom differentiation and the innovation of pathogenesis theory of doctors in past dynasties,it demonstrated the possibility that pathogenesis theory was the innovation breakthrough point of traditional Chinese medicine theory.It proposed the conjecture that pathogenesis was the "saddle point" "sensitive point" of traditional Chinese medicine is theory,and proposed several assume about pathogenesis theory study.

The main component of the tea is tea polyphenols, which can inhibit the processes of carcinogenesis and development of a variety of human cancers by diverse mechanisms. Further research may provide us with more information about the anticancer activities of these promising compounds and facilitate the usage in cancer prevention and treatment.

Objective To investigate the relationship of glycoprotein serglycin (SRGN) expression with invasion and metastasis of breast cancer cells,and the role of microRNA in the regulation of SRGN expression.Methods Real-time quantitative polymer ase chain reaction (PCR) and Western blot were used to detect the differences in SRGN expression between higher metastasis Michigan cancer foundation-7 (MCF-7)/5-Fu breast cancer cell lines and weaker metastasis MCF-7 cell line.The siRNA interference experiment and in vitro Transwell experiment were used to detect effect of SRGN on the ability of invasion and metastasis of breast cancer cells.Bioinformatics software was used to predict miRNAs targeting SRGN,and integrated microRNA differentially expressed chip data between breast cancer cell MCF-7 versus MCF-7/5-Fu.The miRNA quantitative PCR was used to determine the differences of candi date miRNA expression.After transfection of microRNA minics,Western blot was used to test candidate microRNA target SRGN.Transwell experiment was used to test the effects of candidate microRNAs on tumor cell invasion and metastasis.Results SRGN was increased significantly in MCF-7/5-Fu cells,and the invasion and metastasis of tumor cells were inhibited when SRGN was interfered.In addition,miR181 b/c expressed in MCF-7/5-Fu cells was reduced significantly,negatively correlated with SRGN expression,and targeted SRGN expression.It inhibited invasion and metastasis of tumor cells.Conclusions MicroRNA181b/c inhibits metastasis of breast cancer by targeting SRGN.