Animals ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Humans ; Idarubicin ; administration & dosage ; Leupeptins ; administration & dosage ; Neoplasms ; drug therapy ; pathology ; Neoplastic Stem Cells ; drug effects ; metabolism ; pathology ; physiology

Protective effects of chlorpromazine on ischemic heart and its mechanisms were investigated in rats with acute myocardial infarction (MI). 30 SD female rats were divided into three groups. the ischemia group, being subjected MI by coronary ligation, the MI+chlorpromazine group and the control. All samples were taken at 2 hours after operation. The ischemia group, as compared to the control, showed remarkable ultrastructural damage in ischemic myocardium and significant increases in serum CPKmb by 77%, serum cortisol by 67% and serum malondialdehyde (MDA) by 25%(P

The hypoxia tolerance of mice was significantly increased by repetitive action of auto-hypoxia. The tolerance duration of the 2nd, 3rd, 4th and 5th run was 1.8 2.5 3.0 and 3.6 times longer than that of the Ist one. The survival time of mice that had been exposed to hypoxia repeatedly for four runs was 10 times longer than that of the control animals when both of them were placed in the same low pressure chamber and was 4 times longer while KCN was administrated. The survival time under low oxygen pressure in mice injected with brain extract of resistant mice was 1.8 and 2.1 times longer than that of the saline-injected or normal mice's brain extract-injected animals respectively. These results indicate that some plastic or adaptic changes might occur in the tissue cells particularly in the brain cells during acute and repeated hypoxia. They lead the animals' hypoxia tolerance to a very high level. Water soluble antihypoxic or hypoxia-resistant elements might exist in the brain of hypoxia resistant animals, which were extractable, transferable, and permeable to the blood brain barrier.

The present experiment was carried out on mice.The changes in contentsof 12 essential trace elements in the brain of mice were measured by inductivelycoupled plasma emission following acute and repetitive exposure to hypoxia.Followingrepeat exposure to hypoxia for one or two runs, the contents of non-soluble in brainhomogenats as was notably decreased,while that of the soluble V was markedly increased,when compared with that of normal group.When the animals were fed for two daysafter four runs of hypoxia,the contents of As,Fe,Cu,Co,Cr,B,and V were all signi-ficantly or very significantly changed in comparison with the normal value.Further stu-dies are needed to find out the biological importance of these changes and the relationshipbetween them and hypoxia tolerance.

Objective: To study the effect of combining bcr-abl Aspo and c-myb Aspo on K562 cells. Methods: Cellswere exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU-K562 cells were culturedin 0.8% methyleellulose. P210 was measured by flow cytometry. Cellular bcr-abl mRNA was detected by RT-PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr-abl Aspo and c-myb Aspo was 5 μmol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61.7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 μmol/L bcr-abl Aspo and 10 μmol/L c-myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosisat 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr-abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 μmol/L Aspo and 10 mol/L. Conclusion: It emerges coordination to combine bcr-abl Aspo and c-myb Aspo on K562 cells, and enhances the anti-leukemia effect.

Nutritional and metabolic disorders are the common problems in PICU. They often lead to deterioration in child nutrition and vice versa. Reasonable assessment of the energy consumption can provide theoretical basis for proper nutrition support in critically ill children. In this review, the methods of evaluating the energy consumption which included energy metabolism measurement and prediction equations were introduced, and the importance of indirect calorimetry was emphasized. Meanwhile, the research situation of energy consumption assessment at home and abroad and the current status of its clinical application were also reviewed.

Objective To analysis the features of Montreal classification,serum markers,treatment and prognosis of Chinese patients with Crohn's disease(CD),and to estimate the relationships between clinical classification and anti-sarccharomyces cerevisiae antibody(ASCA),treatment and prognosis.Methods A retrospective study of 102 consecutive definite CD eases were performed and all subjects were classified into subtypes according to Montreal classification.The results of ASCA,extra-intestinal manifestation,treatment and disease behavior at follow-up were recorded and compared among different subtypes.Results The A2 subtype(52.9%)was dominated in Chinese CD patients.Ileocolon location(40.2%)and stricture lesion (53.9%)were common.The complication rate was dependent on disease course(P＜0.05),and intestinal fistula was associated with disease location(P=0.074).B1 subtype had higher progressive rate than B2 subtype(P=0.018).ASCA was not associated with disease loeation,disease behavior,treatment and disease progression(P＞0.05).Conclusions Crohn's disease mainly attack young people with male predominance.Early-onset CD patients have higher ASCA positive rate and disease progressive rate.The disease behavior progresses associated with disease course,and the rate of complication and the increase of surgery.The penetrating behavior is the main cause for surgery.Montreal classification is useful to predict the disease course,the need for surgery as well as the prognosis.

Objective To investigate the protection and mechanism of procyanidins (PC) against H2O2 induced oxidative damage of human trabecular meshwork cells (HTMC) in order to provide an experimental foundation for glaucoma clinical treatment.Methods HTMC were cultured and then divided randomly into 5 groups.As untreated group:Normal cultured HTMC;Control group:Normal cultured HTMC + H2O2 (500 μmol · L-1 for 1 hour);Treated group:Normal cultured HTMC + H2O2 (500 μmol ·L-1 for 1 hour) + PC (PC fmal concentrations were 0.02 g · L-1,0.05 g · L-1,0.10 g· L-1).Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to investigate the expression of mitochondrial complex Ⅰ mRNA.Results Compared with untreated group (1.000 0 ± 0.000 0),the differences of mitochondrial complexⅠ mRNA expression in 0.02 g · L-1 PC (0.401 3 ±0.010 3),0.05 g · L-1 PC (0.791 5 ± 0.008 5) groups were statistically significant (all P ＜ 0.01),but the 0.10 g ·L-1 PC group (1.043 0 ± 0.062 2) had no significant differences (P ＞ 0.05).The differences between PC treated groups and control group were statistically significant (P ＜0.01),which showed HTMC treated with PC could increase the expression of mitochondrial complex Ⅰ mRNA.The differences in each PC treated groups were statistically significant (P ＜ 0.01),which showed the expression of mitochondrial complex Ⅰ mRNA were increased along with the concentration of PC gradually increased.Conclusion Exogenetic PC can increase the expression of mitochondrial complex Ⅰ mRNA in the oxidative damaged HTMC,and in a certain range of concentration,the protective effects of PC have the positive relationship of dose-effect,which suggest that PC may be a good candidate for further study of the clinical treatment of glaucoma.

53 male Wistar rats were divided into three groups, i.e. experimental ulcer group, saline control group and normal control group. The antral tissues were prepared for immunohistochemical staining at 4th, 10th, 14th, 21st and 28th days after operation. Sternberger's PAP method was used to demonstrate the gastrin cells (G cells) and somatostatin cells (D cells)in the antral mucosa in order to observe their changes during experimental gastric ulcer. The morphological relationships of G cells and D cells were examined by simultaneous double immunostaining method.The results indicated that the G cells count as well as D cells count in the antral mucosa was increased (P

Studies have shown that cholesterol metabolism plays an important role in tumorigenesis and development.At the same time, it is also shown an important application value in predicting the prognosis of malignant tumors.Cholesterol can improve the long-term quality of life and survival of cancer patients by appropriating risk stratification of these malignant tumors.