Objective To investigate the relationship between plasma cell mastitis and prolactin. Methods The relationship between the lesion area , degree of inverted nipple and prolactin level of 228 plasma cell mastitis patients were observed , as well as their prolactin level before and after treatment , to explore the factors that influence relapse. Results There was no significant statistical relationship between prolactin level and lesions area and degree of inverted nipple. Prolactin level before and after treatment were statistically different (t =2.347,P = 0.02). Menstrual status, nipple status, comorbidities, lesion area and prolactin level were considered, only prolactin level was related with disease recurrence (P = 0.038). Conclusion prolactin level could significantly reduced as the disease cured , but elevated prolactin may lead to relapse of the disease.

Contamination of foodborne pathogens is the main cause of related diseases. Escherichia coli O157:H7 (E.coli O157:H7), as a representative of pathogenic E.coli, is one of the most severe and commonly reported E.coli in the world, but there is still no effective clinical treatment against the infection. Antibiotics show effective in the early infection of E.coli O157:H7. However, their extensive use has led to drug-resistant bacteria and genes in recent years, which becomes a great threat to public health. This article reviews the research progress of E.coli O157:H7 from the prevalence of antibiotic-resistant bacteria, the resistance mechanism, and the prevention and control methods, in order to provide a reference for its prevention, early clinical treatment and related research.

Objective To study the pharmacological effects of Qinggong Decoction on sedation and hypnosis. Methods Fifty mice were randomly divided into five groups: NS group, diazepam group, and low-, mid-, high-dose Qinggong Decoction group. Diazepam group was given 0.1 mL/10 g diazepam, and other four groups were given 0.2 mL/10 g corresponding drugs. Spontaneous activities before and after ig. were recorded and the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and the number of the sleeping mice with pentobarbital sodium (subthreshold dosage) were measured. Convulsion model induced by nikethamide was used to observe the change of the seizure rate in mice after given Qinggong Decoction. Results Qinggong Decoction (3.0, 6.0 g/kg) could obviously inhibit spontaneous activities of mice, prolong the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and increase in the number of the sleeping mice with pentobarbital sodium (subthreshold dosage), and had markedly anticonvulsant effect in mice induced by nikethamide. Conclusion Qinggong Decoction has obvious effects of sedation, hypnosis and anticonvulsion.

ObjectiveTo observe the effect of acupuncture and Chinese medication in improving the quality of life in post-menopausal women.MethodThe menopause Rating Scale (MRS) was adopted to evaluate the quality of life in post-menopausal women. Thirty post-menopausal women of MRS≥20 were randomized into an acupuncture group and a Chinese medication group to respectively receive 4-week treatment. They were scored again by using MRS at the end of intervention and during the follow-up study to compare with the pre-treatment scores.ResultThe decrease of MRS score was more significant in the acupuncture group than that in the Chinese medication group, and the intra-group comparisons between pre-treatment and post-treatment scores showed significant differences (P<0.001), and the intra-group comparisons also showed differences between pre-treatment and follow-up results (P<0.05).ConclusionThe effect of acupuncture in improving the quality of life in post-menopausal women is more significant than that of Chinese medication.

Objective To study the feasibility of tubal reversal after sterilization by laparoscopy.Methods From May 2002 to December 2005,31 patients with tubal ligation received laparoscopic tubal reversal by one-core or two-dot apposition and demixing suture of the serosa.Results The laparoscopic tubal reversal was successful in all the 31 cases.Thirty of the patients were followed up for 8-24 months(mean,16 months);27 of them had intrauterine gestation,3 were non-pregnant;none of them had ectopic gestation.Conclusions Laparoscopic tubal reversal after sterilization can satisfy with the desire and principle of microsurgery.It is feasible for clinical application and has good outcomes.

Objective To investigate the distribution of 16S rRNA methylase genes in Klebsiella pneumoniae strains producing Klebsiella pneumoniae ealbapenenase type 2(KPC-2).Methods A total of 37 Klebsiella pneumoniae isolates producing KPC-2 were collected.The minimal inhibitory concentrations (MICs)of these strains to amikacin,gentamyein and netilmicin were determinated by agal dilution method.Six 16S rRNA methylase genes(armA,rmtA,rmtB,rmtC,rmtD and npmA)were detected by PCR.Results The resistant rates to amikacin,gentamycin and netilmicin were 97.3%(MIC50≥1024μg/mL).Among those resistant strains,8 were armr/A positive,25 were rmtB positive,4 were both armA and rmtB positive.and no other 16S rRNA methylase genes were found.The total positive rate of 16S rRNA methylase genes was 78.4%(29/37).Conclusion 16S rRNA methylase genes armA and rmtB ale prevalent in Klebsiella pneumoniae strains producing KPC-2.

Objective This study aims to test the hypothesis that low density granulocytes (LDGs) is involved in the pathogenesis of DM associated-Interstitial lung disease (ILD).Methods Forty eight DM patients (28 with ILD) and 19 age-and sex-matched healthy Chinese volunteers were recruited to this study.LDGs percentage in peripheral blood mononuclear cells (PBMCs) was tested by flow cytometry.Neutrophilrelated genes (LL-37,MPO and MMP-8) expressions in PBMCs were tested by quantitative RT-PCR.Myositis disease activity assessment visual analogue scales (MYOACT) was used to assess the disease activity.Percentages of LDGs were compared in patients with ILD and without by using unpaired t test with Welch's correction,the correlations between LDGs and clinical parameters were further analyzed by linear correlation analysis.The expressions of neutrophil-related mRNA and proteins in PBMCs were compared by using MannWhitney U test.Results LDGs percentage in PBMCs was 7.1-fold higher in DM patients than healthy controls [(9.1±11.5)％ vs (1.3±0.7)％,t=4.664,P＜0.01].LDGs percentage in PBMCs was 2.7-fold higher in DM patients with ILD than DM patients without ILD [(12.3±14.1)％ vs (4.5±2.6)％,t=2.835,P=0.008 3].The mRNA expression level of LL-37,MPO and MMP-8 and LL-37 protein levels in the DM group were significantly higher than those in the control group.LDGs percentage positively correlated with MYOACT lung disease activity scores (r=0.439,P=0.010).Conclusion Percentage of LDGs in PBMCs is significantly increased in DM patients with ILD and positively correlated with MYOACT lung disease activity scores,suggesting that abnormall increasing of LDGs is a potential contributor to the pathogenesis of DM-associated ILD.

Objective To investigate the incidence and pregnancy outcomes of premature rupture of membranes (PROM) in pregnant women in Beijing.Methods A retrospective multicenter study of 18 534 cases delivered in Beijing Obstetrics and Gynecology Hospital,Beijing Friendship Hospital,Daxing MCH Hospital and Tongzhou MCH Hospital from January 2011 to December 2011,was conducted.Results Among 18 534 cases,PROM occurred in 4 504 cases (24.30％),including 3 910 cases of in term PROM (21.10％) and 594 cases of preterm PROM (3.20％).The incidence of premature delivery was 6.17％ (1 144/18 534),and among 1 144 cases of premature delivery 547 cases were PROM (47.81％);the incidence of PROM was 22.75％ (3 957/17 390) in term delivery.The overall cesarean section (CS) rate was 48.50％ (8 989/18 534) and that in pregnant women with PROM was 35.55％ (1 601/4 504),but the CS rate in pregnant women without PROM was 52.66％ (7 388/14 030).The rate of postpartum hemorrhage was 13.12％ (210/1 601)in CS cases and 4.17％ (121/2 903) in vaginal delivery cases (x2 =121.361,P=0.000).The mean hospital stay for PROM was (5.3±2.9) d in CS cases and (4.3±2.3) d in vaginal delivery cases (t =-12.136,P =0.000).Conclusions Without severe maternal or fetal complications,the incidence of PROM is relatively high in Beijing region and PROM may not increase the maternal or fetal complications.Vaginal delivery is the main mode of delivery for PROM.Cesarean section may not cause less neonatal complications,but may lead to more postpartum hemorrhage and longer hospital stay.

Object To probe into the antihypertension activity mechanism of extracts from Compound Jueming (CJ). Methods The method of radioimmunoassay was used to assay the concentration of the related substances on spontaneously hypertensive rats (SHR) such as angiotensin Ⅱ (ANGⅡ), renin activity (RA), endothelin (ET), atrial natriuretic polypeptide (ANP), aldosterone (ALD), and urine. Results All of the three doses (9.5, 7.2, and 4.8 g/kg) of extracts from CJ reduced ANG Ⅱ and RA, but had no influence on ALD, ET, ANP, and urine in SHR. Conclusion The antihypertension effect of extracts from CJ is related to decreasing of renin angiotensin, but not to ALD, ET, ANP, and urine.

BACKGRoUND:The clinical manipulation properties of light-cured composite resin,such as flowing property.filling capacity,shaping and stability has not unified definition in dental materials notwithstanding more studies of rheological behaviors were undertook.OBJECTIVE:To explore the dynamic viscoelasticity difference of unpolymerized light.cured composite resins with retentive filler or normal filler,and to investigate effect on the clinical manipulation properties of rheological behaviors in two light-cured composite resins to define an ideal resin.DESIGN,TIME AND SETTING:This controlled study is a correlation study of integrating the base study and clinical use.Basic study was performed in January 2003 at Key Laboratory of Engineering Plastics of Chinese Academy of Sciences in Beijing.The clinical observation study was conducted at the Stomatology Center,Haikou People's Hospital until December 2006.MATERIALS:Light-cured composites employed in this study were reinforced with RF(experimental composite resin I,ECR-Ⅰ)or NF(experimental composite resin Ⅱ,ECR-Ⅱ),which were supplied by Dental Materials Laboratory.Medical College of Stomatology of Peking University.They contained different types of fillers,but had the same resin matrix and volume percent of filler.METHODS:The dynamic viscoelastic properties of ECR-Ⅰ or ECR-Ⅱ were measured respectively with the dynamic stress rheometer DSR-200 in a room maintained 25℃.Flowing property,filling capacity,shaping and stability property were employed to assess the clinical manipulation property of ECR.MAIN OUTCOME MEASURES:Viscoelasticity(G',G",tan δ)and clinical manipulation of two kinds of compound resins.RESULTS:The dynamic viscoelastic behavior of the two composites was sensitive to changes in frequency.ECR-Ⅰhad significantly higher G'than ECR-Ⅱ.ECR-Ⅰ had a greater ability of stability and shaping.and a lower potential for deformation clinically.ECR-Ⅱ had significantly higher G"than ECR-Ⅰ.and ECR-Ⅱ had a higher flowing ability clinically.ECR-Ⅱ had significantly higher tan δ than ECR-Ⅰ.ECR-Ⅱ was found to be more viscous and better filling capacity,with a good adaptation to the cavity of the tooth clinically.CONCLUSION:The dynamic viscoelasticity(G',G",tan δ)and clinical manipulation properties of ECR-ⅠI or ECR-Ⅱ are compared.The ideal composites should have higher G'and lower tan δ