Objective:To study the expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in fresh and two preserved amniotic membranes (AM),and to explore their biological vitality.Methods:The amniotic membranes from the healthy puerperia with abdominal delivery were randomly divided into three groups:fresh group,DMEM/glycerol preserved group at -80℃ and glycerol preserved group at 4℃ (for 1 and 3 months).Immunohistochemical method was used to investigate the changes in the expressions of HGF and bFGF among the three groups.Results:HGF and bFGF were distributed in the epithelium of AMs mostly.The expressions of HGF in both preserved AMs for 1 and 3 months decreased significantly compared with fresh AM (P

Humans ; Multiple Myeloma ; Neoplasm Recurrence, Local

?AlM: To observe the changes of corneal topography and astigmatism et al with various duration of closing eyes and sleep;and study the impact of closing eye and sleep causing physiological hypoxia on corneal topography.
? METHODS: Sixteen volunteers were selected ( 32 eyes), 22-33 (26. 19 ± 3. 95) years old, without refractive errors and other eye diseases, as well as never wearing corneal contact lens, including 10 male eyes and 22 female eyes. The main parameters received corneal simulation K value, corneal astigmatism, corneal irregular measure ( ClM ) and the shape factor ( SF ) by Zeiss corneal topographer. They were measured before and after closed eyes 10, 20, 30, 60, 120min; before sleep and after over 6h sleep, they were detected immediately as soon as they opened eyes after 10, 20, 60, 120min.
? RESULTS: During closing eyes, there were no statistical significance on the changes of corneal topography, corneal astigmatism, and SF. After opening eyes and over 6h sleep, there were no statistical significance on the changes of corneal topography, corneal astigmatism, ClM, and SF.
?CONCLUSlON:Closing eyes and sleep don’t injure the cornea substantially, so corneal topography and corneal astigmatism don’t mainly change.

AIM: To investigate the effect and mechanism of Mingjing decoction combining with argon laser on the levels of plasma vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy(DR).
METHODS:The 66 patients(118 eyes)with DR at Ⅲ ~ Ⅴstage were randomly divided into two groups (treatment group and control group). The control group (33 patients with 56 eyes ) was treated with argon laser, and the treatment group (33 patients with 62 eyes) was treated with mingjing decoction combining with argon laser, and a blank group(30 eyes) was set up. The levels of plasma VEGF were detected by enzyme linked immunosorbent assay (ELISA). The results of best corrected visual acuity and fundus fluorescein angiography(FFA) were analyzed, as well as detected glycosylated hemoglobin ( HbA1c), blood coagulation function and the function of liver and kidney.
RESULTS: Patients with DR had significantly risen plasma VEGF before treatment when compared with blank control group ( P < 0. 05 ). After 3mo of combined treatment, the levels of plasma VEGF significantly reduced and HbA1c decreased in patients treated with mingjing decoction and argon laser, there were statistically significant difference compared to control group ( P < 0. 05 ). In the treatment group, the best corrected visual acuity and FFA were significantly improved in patients treated with mingjing decoction combining with argon laser compared to patients treated with argon laser alone after 3mo of combined treatment. CONCLUSION: Mingjing decoction combining with argon laser for DR can effectively reduce the level of plasma VEGF, stabilize blood sugar levels, improve the function of retina, and delay DR progresses.

Asthma, a complex polygenetic disease, has individual differences in patient′s response to asthma drugs,which is mainly related to gene polymorphism. These genes include beta 2-adrenaline receptor genes(ADRB2),the glucocorticoid receptor genes(NR3C1) and leukotriene synthase genes(LTC4S and ALOX5),etc. Pharmacogenomics can realize individualized treatment of asthma,reduce the admission rate and the mortality rate,and reduce the cost of treatment. Pharmacogenomics of children asthma is reviewed in this arti-cle,hoping to provide help for the individualized treatment of asthma.

Objective To observe the clinical efficacy of acupuncture-moxibustion in treating endometriosis (EM). Method Fifty EM patients were randomized into two groups, 25 cases in each group. The treatment group was intervened by acupuncture-moxibustion, while the control group was intervened by oral administration of Mifepristone tablets. Abdominal pain, pelvic lump, and relevant indicators were observed before and after the treatment, and the therapeutic efficacies were compared.Result After 6-month treatment, symptoms including abdominal pain obviously subsided in the treatment group and the total effective rate was 92.0%, versus 52.0% in the control group, and the between-group difference was statistically significant (P<0.01). The pelvic lump size was significantly changed after the treatment in both groups (P<0.05), suggesting that the lump size became smaller in both groups after the treatment;there was a significant difference in comparing the pelvic lump size between the two groups after the treatment (P<0.05), indicating that the lump size was reduced more significantly in the acupuncture-moxibustion group. The level of serum CA125 changed significantly after the treatment in both groups (P<0.05), suggesting that serum CA125 dropped in both groups after the treatment; there was a significant difference in comparing the level of serum CA125 between the two groups after the treatment (P<0.05), indicating that serum CA125 dropped more significantly in the acupuncture-moxibustion group. A year later, there was a significant difference in comparing the relapse rate between the two groups (P<0.05), revealing that the relapse rate was significantly lower in the acupuncture-moxibustion group than in the medication group.Conclusion Acupuncture-moxibustion can produce a marked therapeutic efficacy in treating EM.

Objective To investigate the risk factors of ischemic cerebral stroke after transient ischemic attacks (TIAs).Methods The clinical data of 184 TIA patients were collected.The risk factors of ischemic cerebral stroke after TIA were analyzed.Results Compared with no ischemic cerebral infarction group, the rate of elderly (≥60 years old), hypertension, diabetes, smoking and drinking history in ischemic cerebral infarction group (P<0.05-0.01).There was no statistical significance in gender and hyperlipidemia between the two groups (all P>0.05).Compared with no ischemic cerebral infarction group, the rate of attack time≥30 min, attack frequency≥3, course of disease≥24 h were significantly increased (P<0.05-0.01).There was no statistical significance in TIA types between the two groups (all P>0.05).Logistic regression analysis showed there were a positive correlations between elderly, hypertension, diabetes, smoking and drinking history and ischemic cerebral stroke after TIA (OR=29.799, 95%CI: 2.189-405.569, P=0.011;OR=0.649, 95%CI: 0.038-6.850, P=0.005;OR=8.569, 95%CI: 1.314-55.862, P=0.025;OR=0.158, 95%CI: 0.025-0.980, P=0.048).Conclusion Elderly, hypertension, diabetes, smoking and drinking history are independent risk factors for ischemic cerebral stroke after TIA.

Objective To observe the therapeutic efficacy of acupuncture plus moxibustion in treating chemoradiotherapy-induced leukopenia in patients with malignant tumors.Method A total of 120 patients with malignant tumors who presentedleukopenia during receiving chemoradiotherapy were randomized into two groups. Sixty cases in the treatment group were intervened by acupuncture plus moxibustion; sixty cases in the control group were intervened by oral administration of Batilol tablets and Leucogen tablets.The peripheral white blood cell (WBC) count in the two groups was detected on treatment day 3, 7, 14, 21, and 28.The clinical efficacies of the two groups were compared.ResultThe WBC started toincreaseafter 1-week treatment in thetreatment group (P<0.05) and the increase was statistically significant after 2-week treatment (P<0.01); in the control group, the WBC started to increase after 2-week treatment (P<0.05)and the increase was statistically significant after 3-week treatment (P<0.01); the effect of increasing WBC was more significant in the treatment group compared to the control group (P<0.05,P<0.01).The total effective rate was 90.0% in the treatment group versus 53.3% in the control group, and the between-group difference was statistically significant (P<0.01).Conclusion Acupuncture plus moxibustion can produce a significant efficacy in treating leukopenia induced by chemoradiotherapy.

OBJECTIVE:To study the anti-hepatofibrotic action of 18?-glycyrrhizic acid(18?-GL) and gamma interferon (IFN-?) in rats METHODS:The rat model of liver fibrosis was induced by dimethyl nitrosamine(DMN),18?-GL or IFN-? was administrated before and 5 weeks after giving DMN,to prevent and treat liver fibrosis The pathological changes and degree of fibrosis in the liver were observed and compared with that of DMN control group RESUTLS:Compared to DMN control group,the collagen deposition in both 18?-GL and INF-? groups was significantly slight in degree(P