The common rheumatic diseases in children including rheumatic fever,juvenile idiopathic arthritis,systemic lupus erythematosus (including neonatal lupus),juvenile dermatomyositis,Kawasaki disease,anaphylactoid purpura.Rheumatic diseases as a group of unknown etiology autoimmune diseases,connective tissue and collagen fibers of different organs can be affected.Systemic inflammatory is the common prominent characteristics of this kind of disease,often resulting in multiple organ damage.Over recent decades,there has been considerable interest in the long-term outcomes of individuals with chronical inflammatory disease and an area of particular concern has been the increased prevalence of cardiovascular disease.Since sustained systemic inflammation is known to accelerate atherosclerosis,doctor should pay attention to rheumatic diseases associated cardiovascular involvements during daily clinical work.The cardiovascular involvement of common rheumatic diseases in children is summarized in this paper.

Eighty percent of unexplained syncope in children is neurally mediated syncope (NMS).The cur-rent clinical diagnosis of children NMS mainly depends on the head -up tilt test(HUTT),which has a certain risk, therefore searching for a safe noninvasive NMS diagnosis method is of great significance.Twenty -four -hour ambulato-ry blood pressure monitoring (ABPM)is widely used in clinic recently.It was reported that there was autonomic nerve adjustment imbalance in children with NMS.And the diagnostic value of ambulatory blood pressure pattern to NMS chil-dren was high.Non -spoontype blood pressure variation pattern of NMS could aid to diagnose children NMS.Fur-therly study of 24 h ABPMof NMS children has great significance to explore the pathogenesis of the NMS,to be benefi-cial to explain the regulating mechanism of the vessels,and go a step further to guide the treatment.

Objective To set up a stable method to induce SW872 adipocyte to differentiation.Methods THE animals were randomly divided in to five groups according to different inducing agents and a control groups.The changes of the cells were observed by microscopy and Oil-Red O staining.TG mass of these cells were assayed by chemical colorimetry methods.Results The oleic acid is the strongest factor to induce SW872 cell to differentiate,IBMX+DEX+Insulin is also strong factor,but it took a longer time to act.The effect of IBMX is stronger than insulin and ASP,but less than Oleic acid and IBMX+DEX+Insulin.Oleic acid significantly increased triglyceride mass of SW872 cell on the 1~(th) day of differentiation,The TG mass in the oleic acid group increased on the 2~(th) and4~(th)day of differentiation. Conclusion Oleic acid is the best Inducer to stimulate SW872 cell to differentiate,it can induce SW872 preadipocytes to differentiate to adipicytes in a short term.

Objective To study the genetic polymorphisms of STR locus D20S85 and to obtain the genetic data of Guangdong Han, Guangxi Zhuang, Huadong Han,Neimenggu Han and Meng populations. Method Using PCR and silver - staining methods, the alleles of D20S85 locus were detected and analysed. Results 9 alleles and 35 genotypes were found in those populations. The highest allele frequency was D20S85* 6. The results of Hardy - Wein-berg equilibrium test showed that the genotype distributions observed in five populations were correspondent with the expected. The expected heterozygosity was 0.7720 - 0.7912; the exclusion probability of paternity was 0.7538-0.7594(triplet) and 0.3988 -0.4297 (biplet) ; the discrimination power 0.9175 -0.9272 and the polymorphism information content 0.7442 - 0.7656. Conclusion STR D20S85 locus is a useful genetic marker for forensic application .

Objective To investigate the STR typing discordance between different typing methods.Methods Genotypes of 13 routine forensic STR loci in DNA samples from 100 individuals were typed by using singleplex polyacrylimide gel electrophoresis silver stain system and Power Plex16 System,respectively.The typing results between these two systems were compared.Results One genotype discordance was observed at D8S1179 locus in a DNA sample.The genotypes was 12/14 in snigleplex system and 12/15 in Power Plex16 System.Conclusion Different STR typing systems may result in different genotyps from the same DNA sample.

Objective To investigate the polymorphism of DXS6854 locus in Guangdong Han population. Methods The DXS6854 locus was analyzed by PCR following polyacrylamide gel electrophoresis and silver staining. Results Among 189 females and 230 males from Guangdong Han population, 8 alleles were observed with frequencies ranging from 0.0026 to 0.4522. Exact tests demonstrated genotype frequencies in females had no departure from Hardy- Weinberg equilibrium. The discrimination powers for female and male original samples were 0.8633 and 0.7012, respectively. When both mother and her daughter were tested, the probability of excluding a random man as a father was 0.6712. Conclusion The DXS6854 locus is appropriate for individual identification and paternity testing involving a female child.

Objective To investigate the clinical and imageological features of pituitrin-induced delayed encephalopathy. Methods Clinical data of 8 patients with delayed encephalopathy caused by pituitrin were analyzed retrospectively. Results All of the 8 patients presented diffent degree neuropsychic symptoms at 4～12 d after stop using the pituitrin. The extrapyramidal and psychiatric symptoms of the cases were found,such as hypermyotonia(8 cases),hypokinesia(6 cases),extremity buffeting(3 cases),emotional and behavior disorder(6 cases). The 8 cases with EEG examination showed:there were gently to midrange widespread dysfunction in 4 cases,severe widespread dysfunction in 1 case. The levels of serum Na+ in 5 cases were decrease slightly. The 8 cases with brain MRI examination showed that the abnormal signals were mainly located in lentiform nucleus and head of caudate nucleus with long T1 and T2-weighted images,and including thalamus or midbrain abnormalities signal in 1 case,respectively. Conclusions The manifestations of pituitrin-induced delayed encephalopathy are extrapyramidal symptoms and cerebral disorders. The characteristics of brain MRI are abnormal signals in lentiform nucleus and head of caudate nucleus with long T1 and T2-weighted images. The supposed pathogenesis may be nerve necrosis induced by Charcot's artery spasm and hyponatremia.

After the development history, knowledge system and main characteristics of Introduction to medical in-formatics textbooks in China were described, the problems in the textbooks used at present were analyzed, and sug-gestions were put forward for their improvement.

Objective To evaluate the efficacy of entecavir treatment in chronic hepatitis B virus ( HBV ) infected patients with mild hepatic dysfunction and marked pathological injury.Methods One hundred and fifty five chronic hepatitis B ( CHB) patients with HBV DNA>1.0 ×104 U/mL admitted in Lishui People’ s Hospital during January 2008 to October 2011 were enrolled in the study.Patients were divided into three groups: those with serum ALT <2 ×ULN and liver inflammation injury ≥G2 and/or fibrosis stage≥S2 were in observation group ( n=75 ); patients with ALT ( 2-5 ) ×ULN were in control group 1 (n=38);patients with ALT>5 ×ULN were in control group 2 (n=42).All patients were given entecavir (0.5 mg, 1/d, p.o) treatment.ALT normalization rates, HBV DNA negative rates, HBeAg negative conversion rates and seroconversion rates at 12-, 24-,48-, 96-and 144-week were observed and compared among groups.Variance analysis andχ2 test were performed for measurement data and numeration data, respectively.Results ALT normalization rates in observation group were 86.7%, 90.7%, 90.7%, 92.0%and 96.0%at 12-, 24-, 48-, 96-and 144-week, which were higher than those in control group 1 (χ2 =2.04, 2.15, 2.78, 2.69 and 2.47, P <0.01), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.53,2.42,2.09,2.24 and 2.32,P>0.05) . HBV DNA negative rates in observation group were 70.7%, 78.7%and 82.7%at 12-, 24-and 48-week, which were higher than those in control group 1 (χ2 =4.56, 4.23 and 4.28, P<0.05), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.75, 2.62 and 2.98, P>0.05).HBeAg negative conversion rates in observation group were 6.6%, 21.3%and 25.3%at 48-, 96-, and 144-week, which were higher than those in control group 1 (χ2 =4.68, 4.78 and 5.01, P<0.05), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.24, 2.57 and 2.13, P>0.05).HBeAg seroconversion rate in observation group was 4.0%at 24-week, which were higher than that in control group 1 (χ2 =2.87, P <0.05), but the seroconversion rates at 96-and 144-week were lower than those in control group 2 (χ2 =2.92 and 3.14, P<0.05).Conclusion The efficacy of entecavir treatment for HBV infected patients with mild hepatic dysfunction and marked pathological injury is satisfactory.

The extended service in domestic and foreign medical libraries was summarized according to the trans-formation of medical library and the need of public health information followed by a description of some typical cases of extended service provided by MedlinePlus website , Disaster Medical Information Management Research Center , and patient libraries in order to provide the reference for domestic medical libraries to cary out their extended service .