Objective: To investigate psychosexual health of students during pubertal transition in Taizhou and explore the influence of family factors. Methods: A questionnaire survey was conducted among 1 215 students in senior grades of primary school, junior and senior high schools in Taizhou by stratified cluster sampling. Results: The average score of adolescents sexual and mental health questionnaire was (3.82±0.52), the sexual knowledge score was (3.31±1.00) and the score of sexual adaptation was (4.29±0.68). Sexual attitude and sexual control among female students were significantly better than male students (t=4.53,6.96，P<0.01). The four dimensions of physiological knowledge, sexual knowledge, sexual concept and self-adaptation made significant progress with the improvement increased with educational background(F=157.18，85.18，85.96，65.54，P<0.01). The parent-child relationship, parental sex education, the only child in the family, education background of parents, family economic status and the long-term separation from parents in early childhood had predictive effects on the development of puberty students’ psychosexual health (P<0.05). Conclusion Students during pubertal transition in Taizhou show moderate level of psychosexual health. Schools should strengthen sexual knowledge education.In order to promote students’ psychosexual health, more attention should be paid to the influence of family factors.