In order to investigate the mutation of HBV polymerase gene reverse transcription conserved region (P region) in chronic hepatitis B (CHB) patients, 212 CHB patients who took antiretroviral treatment with nucleotide analogues were chosen. The drug resistance mutations of HBV P region and HBV genotype were detected by Pyrosequencing. Sequence analysis showed that the drug resistance sites of HBV P region located at sites 173; 180; 181; 184; 204; 236 and 250. The main site of HBV P region drug resistance was 204 and 180, accounting for 35.8% and 23.5%, respectively. There were significant differences in the mutation rate of site 180 among different age groups. There were also significant differences in the mutation rate of site 204 among younger than 30 age group, 41 to 50 age group and 51 to 60 age group. (P < 0.05, P < 0.01). The mutation rate of site 180 combined with site 204 was 66.6%. The mutation rate of site 181 combined with site 236 was 23.3%. The age of C genotype infected patients was significantly older than B genotype infected patients (P < 0.01). M204V/I mutation mostly existed in the form of joint L180M mutation, the mutation rate was age-related. The detection of HBV genotypes and drug resistance sites of HBV P region have important clinical implications for the treatment and prognosis of patients with CHB.
Adult ; Aged ; Antiviral Agents ; pharmacology ; China ; Drug Resistance, Viral ; Female ; Gene Products, pol ; genetics ; Genotype ; Hepatitis B virus ; classification ; drug effects ; enzymology ; genetics ; Hepatitis B, Chronic ; drug therapy ; virology ; Humans ; Male ; Middle Aged ; Mutation, Missense ; Young Adult

Cardiovascular Diseases ; prevention & control ; China ; Humans

Cardiovascular Diseases ; etiology ; prevention & control ; Humans ; Metabolic Syndrome

Objective To evaluate the correlations of red blood cell distribution width (RDW) and short term prognosis of patients with acute coronary syndrome.Methods 179 patients with acute coronary syndrome (ACS) treated in our hospital between Jan.2008 to Apr.2009 were grouped by cutoff value of 14.4%.The incidence of acute myocardiac infarct( AMI),malignant arrhythmia,heart falure,sudden cardiac death(SCD) were compared in the two groups when patients were hospitalized and in half years of discharged.High-sensitivity C-reactive protein.(hs-CRP) ,BNP were also compared between the two groups.Results The incidences of cardiac adverse event were different between the two groups(AMI:39.47% vs.15.53% ,HF:22.22% vs.6.80% ,SCD:9.21% vs.1.94% and malignant arrhythmia:11.84% vs.3.90%,P values all less than 0.05 ).hs-CRP were ( 9.44 ± 8.03 ),(4.64 ±4.42) mg/L and BNP were (357.07 ± 161.60),(233.17 ± 134.76) ng/L in the two groups ( P ＜ 0.05 ).RDW is positively correlated with hs-CRP and BNP (r is 0.42 and 0.58 respectively,P-value all less than 0.05).Conclusions The RDW is related to the short term progonosis in patients with ACS,and also positively correllated with plasma hs-CRP and BNP levels.

Objective To investigate the curative effect of Aidi injection solution combined withcarboplatin on controlling malignant pleural effusion(MPE).Method A total of 86 cancer patients with appearedPME were selected as our subjected who hospitalized in the First People's Hospital of Shangqiu from Feb.2012to Mar.2013.They were randomly divided into the observation group(43 cases) and the control group(43cases).Patients in the oontrol group were treated with carboplatin at dose of 100 mg/per times which was putinto the thoracic cavity,and patients in the observation group was treated with Aidi injection solution at dose of100 mg/per times beside carbeplatin.The treatment period was 4 weeks.The effect information,quality of lifeand adverse reactions were recorded.Results The total effective rate and survival quality improvement rate inobservation group were 86.05％ (37/43) and 51.16％ (22/43),higher than those in control group (67.44％(19/43),30.23％ (13/43)),and the differences were significant(x2 =4.170,3.903 ; P ＜ 0.05).There wasno significant difference in terms of the related adverse reactions between two groups(P ＞0.05).ConclusionThe therapy of Aidi injection solution plus carboplatin on controlling MPE is proved to be effective and it canimprove patients' quality of life with better safety.Therefore it is worthy of recommendation.

Objective:To explore the therapeutic value of atorvastatin combined bezafibrate on adjusting lipid in pa‐tients with acute coronary syndrome (ACS) .Methods :A total of 121 ACS patients who were treated in our hospital from Apr 2013 to Sep 2013 were enrolled .According to therapeutic method ,they were divided into bezafibrate group (n=60 ,received bezafibrate treatment based on routine treatment ) and combined treatment group (n=61 , received additional atorvastatin based on treatment of bezafibrate group ) .Levels of total cholesterol (TC) ,triglyc‐eride (TG) ,low density lipoprotein cholesterol (LDL‐C) and high density lipoprotein cholesterol (HDL‐C) ,stand‐ard‐reaching rate of blood lipid and incidence rate of adverse reactions were compared between two groups before and after treatment .Results:Before treatment ,there were no significant difference in all blood lipid levels between two groups , P> 0.05. Compared with before treatment ,levels of TC ,TG and LDL‐C significantly reduced and HDL‐C level significantly rose in both groups after treatment , P< 0.05 or < 0.01 ;Compared with bezafibrate group ,there were significant reductions in levels of TC [ (4.06 ± 0.49) mmol/L vs .(3.35 ± 0.29) mmol/L] ,TG [ (1.60 ± 0.33) mmol/L vs .(1.12 ± 0.21) mmol/L] and LDL‐C [ (2.18 ± 0.39) mmol/L vs .(1.91 ± 0.26) mmol/L] ,and significant rise in HDL‐C level [ (1.09 ± 0.21) mmol/L vs .(1.26 ± 0.26) mmol/L] ,P<0.05 all;and sig‐nificant rise in standard‐reaching rates of TC (41.67% vs .67.21% ) ,TG (61.67% vs .85.25% ) ,LDL‐C (36.67%vs .70.49% ) and HDL‐C (41.67% vs .90.16% ) in combined treatment group after treatment ,P<0.05 all .There was no significant difference in incidence rate of adverse reactions (11.48% vs .10.00% ) between combined treat‐ment group and bezafibrate group , P>0.05. Conclusion:Atorvastatin combined bezafibrate can significantly im‐prove blood lipid condition and possess good safety in ACS patients .

Diabetic ketoacidosis (DKA) and hyperglycemic hyperosmolar status (HSS) are severe acute diabetic complications. This article mainly discusses the definition, mortality rate, pathophysiology, precipitating factors, main clinical manifestations, laboratory findings and principles of treatment in DKA and HHS.

OBJECTIVE:To establish a method for the bacterial endotoxin test of chlorphenamine maleate injection. METHODS: The interference test and bacterial endotoxin test of 28 batches of chlorphenamine maleate injections from 14 manufacturers were carried out in accordance with the bacterial endotoxin test approved by Chinese Pharmacopeia (2005 edition). RESULTS: Diluted to a concentration of below 0.025 mg?mL-1,the samples showed no interference action on bacterial endotoxin test. The limit value of the bacterial endotoxin was 5 EU?mg-1. The results of the bacterial endotoxin test of the 28 batches of samples were all accorded with the standard of the established quality control. CONCLUSION: The established TAL method is applicable for bacterial endotoxin test of chlorphenamine maleate injection.

Objective To study the role of ARHI protein and COX-2 protein and to explore their correlation in pathogenesis of adenocarcinoma of the uterine cervix.Methods The expression of ARHI and COX-2 protein were detected both in 40 cases of adenocarcinoma of the uterine cervix and in 30 cases of normal cervix by immunohistochemistry.Results The positive rate of the expression of ARHI protein in adenocarcinoma of the uterine cervix was 50.0％ (20/40),which lower than that in normol cervix controls 90.0％ (27/30),the difference was significant ( x2 =12.43,P ＜ 0.05 ) ; The positive rate of the expression of COX-2 protein in adenocarcinoma of the uterine cervix was 82.5 ％ (33/40),which higher than that in normol cervix controls 0 (0/30),the difference was significant (x2 =46.16,P ＜ 0.05 ) ;The positive expression rate of ARHI was negatively correlated with the clinical stage of cervical adenocarcinoma ( x2 =3.96,P ＜ 0.05 ) disease and pathological grade (x2 =7.04,P ＜ 0.05 ).COX-2 protein was positively correlated with pathological grade (x2 =7.04,P ＜0.05 ),but had no correlation with the clinical stage of cervical adenocarcinomacervical adenocarcinoma stage of diseas(P ＞ 0.05) ;The expression of ARHI protein and COX-2 protein wasn't correlated with lymph nodeatic meatastasis(P ＞ 0.05);The expression of ARHI protein had correlation with the expression of COX-2 protein (r =0.31,P ＜ 0.05 ).Conclusion Inactivation of ARHI gene and the abnormal expression of COX-2 protein are involved in the origin of cervical adenocarcinoma,their synergistic reaction may play an important role in tumor origin of cervical adenocarcinoma.

OBJECTIVE　A HPLC method was established for the determination of racecadotril and its impurities.METHODS　A ODS column was used and the mixture of acetonitrile-potassium dihydrogen phosphate (KH2PO4) solution (70∶30) was used as the mobile phase.The detection wavelength was 210 nm.RESULTS　The liner range of the racecadotril was 0.08～0.24 mg*mL-1 and the regression equation was Y=15847X+3873(r=0.9997).The liner range of benzylthiorphan disulphide was 2.40～21.56 μg*mL-1 and the regression equation was Y=1826x+46 (r=0.9999).The measurable lowest limit was 1 μg*mL-1.The average recovery was 100.0%.CONCLUSION　The method was convenient,accurate and specific.