Objective To explore tetrandrine on murine hemangioendothelioma cell and its mechanisms.Methods Explore the effect of tetrandrine on EOMA cells in time and concentration MTT experiment, the content of tetrandrine treated EOMA cells in different cell cycle detected by flow cytometry, speculated effect of tetrandrine on G1/S phase checkpoint for EOMA cells blocking.Western blot analyzed the molecular mechanism of tetrandrine induced EOMA cell block, and intracellular reactive oxygen species level were detected byflow cytometry.Then used the active oxygen scavenger NAC pretreated cells after drug incubation.Results Tetrandrine could inhibit the cell reproduction, and showed a concentration and time dependence, role in tetrandrine 20μM was significantly inhibited after 48 h.Tetrandrine caused mainly by EOMA cells during the cell cycle of G1/S phase cell block, so as to achieve the effect of inhibiting cell proliferation.Tetrandrine increased intracellular reactive oxygen species ( ROS) blockade effect of elevated levels of cell cycle to achieve, and the regulation of AKT, GSK-3 βand p53 cell cycle upstream protein level, finally maked the expression changes.Further research proved that, tetrandrine induced up-regulation of ROS level in cells to induce EOMA cell cycle arrest.Moreover, regulated by the ROS inhibitor NAC could significantly inhibit EOMA cell cycle factor.Conclusion Tetrandrine can inhibit tumor angiogenesis, which provides theory basis for clinical anti-cancer drugs in inhibiting tumor growth.

Many studies had shown that the stigma is the important factor for diagnosis,treatment and re-covery of bipolar disorder.The stigma not only has negative effects on the patients and also on the marriage,fami-lies,study,work,medication treatment,and many other aspects.This paper reviews the development of definition of stigma,the characteristics,effects,interventions of stigma of bipolar disorder,finally,make a summary and put for-ward the prospects.

Objective] Summarize the pathogenesis of asthma and leukotriene receptor in latest research situation. [Methods]Check out the past five years CNKI, VIP data, PUBMED data to leukotrienes and asthma for keyword research literature, and classification, summary. [Results]Numerous clinical studies, in vivo and in vitro experiments showed that leukotrienes and their receptors are involved in the pathogenesis of asthma and progress in a multi-cell, multi-session, and interact with a variety of cytokines.[Conclusion]With concept of wholism as the characteristics of the Genomics technologies, more suitable as a primary means of application in the field of medical research has an overall concept for the regulation of traditional Chinese medicine research in the field of drugs to treat asthma leukotriene pathway and provide a new perspective.

Objective:To explore the therapeutic value of atorvastatin combined bezafibrate on adjusting lipid in pa‐tients with acute coronary syndrome (ACS) .Methods :A total of 121 ACS patients who were treated in our hospital from Apr 2013 to Sep 2013 were enrolled .According to therapeutic method ,they were divided into bezafibrate group (n=60 ,received bezafibrate treatment based on routine treatment ) and combined treatment group (n=61 , received additional atorvastatin based on treatment of bezafibrate group ) .Levels of total cholesterol (TC) ,triglyc‐eride (TG) ,low density lipoprotein cholesterol (LDL‐C) and high density lipoprotein cholesterol (HDL‐C) ,stand‐ard‐reaching rate of blood lipid and incidence rate of adverse reactions were compared between two groups before and after treatment .Results:Before treatment ,there were no significant difference in all blood lipid levels between two groups , P> 0.05. Compared with before treatment ,levels of TC ,TG and LDL‐C significantly reduced and HDL‐C level significantly rose in both groups after treatment , P< 0.05 or < 0.01 ;Compared with bezafibrate group ,there were significant reductions in levels of TC [ (4.06 ± 0.49) mmol/L vs .(3.35 ± 0.29) mmol/L] ,TG [ (1.60 ± 0.33) mmol/L vs .(1.12 ± 0.21) mmol/L] and LDL‐C [ (2.18 ± 0.39) mmol/L vs .(1.91 ± 0.26) mmol/L] ,and significant rise in HDL‐C level [ (1.09 ± 0.21) mmol/L vs .(1.26 ± 0.26) mmol/L] ,P<0.05 all;and sig‐nificant rise in standard‐reaching rates of TC (41.67% vs .67.21% ) ,TG (61.67% vs .85.25% ) ,LDL‐C (36.67%vs .70.49% ) and HDL‐C (41.67% vs .90.16% ) in combined treatment group after treatment ,P<0.05 all .There was no significant difference in incidence rate of adverse reactions (11.48% vs .10.00% ) between combined treat‐ment group and bezafibrate group , P>0.05. Conclusion:Atorvastatin combined bezafibrate can significantly im‐prove blood lipid condition and possess good safety in ACS patients .

Objective The BOLD -fMRI(blood oxygenation level dependent functional magnetic resonance imaging)was used to observe the functional cortical activities of the bilateral cerebral hemispheres in 23 cases of chil_dren with congenital sensorineural hearing loss under resting state ,and to further explore the values of BOLD-fM_RI in patients with sensorineural hearing loss .Methods Twenty three children with congenital sensorineural hearing loss were included in the experimental group and 10 normal heating children were included as cronol group .The ALFF(amplitude of low -frequency fluctuation) method was used to analyze the image data ,and to compare the ex_perimental and control groups with bilateral cerebral cortex function activities for the quantitative analysis .ResuIts The resting state activity intensity in the left inferior temporal gyrus ,left fusiform gyrus ,right cingulate gyrus and left post central gyrus of the experimental group were significantly greater than those of in the corresponding intensi_ty cortex of the control group (P< 0 .01) .ConcIusion The cortex of sensory systems in children with congenital sensorineural deafness may have functional remodeling ,cerebral cortex of patients with sensorineural hearing loss may have perception compensation phenomenon .

Objective: To observe the clinical effects of comprehensive treatmenton cutaneous region for low back pain.
Methods: One hundred and twenty outpatients with low back pain who met the diagnostic criteria were randomly divided into a cutaneous region group or a medication group, 60 cases in each group. The cases in the cutaneous region group were treated by Nie-pinching up the skin of the lumbosacral region, cupping and acupuncture. Those in the medication group were treated by oral administration of Celecoxib capsule. The visual analogue scale (VAS) and Oswestry disability index (ODI) were used to assess the therapeutic effects.
Results: After treatment, the VAS scores of both groups were different from those before treatment, showing statistical significances (allP<0.01). The D-valuse of VAS scores of both groups were different from those before treatment, showing statistical significances (allP<0.01). The D-value of OID scores in the cutaneous region group was significantly different from that in the medication group(P<0.01). After treatment, the total effective rate was 91.7% in the cutaneous region group versus 76.7% in the medication group, there was a significant difference in comparing the clinical effect (P<0.01).
Conclusion: Both comprehensive treatment on the cutaneous region and Celecoxib capsule can obviously relieve low back pain. But comprehensive treatment on the cutaneous region is better than Celecoxib capsule in the therapeutic effects.

Objective To investigate the curative effect of Aidi injection solution combined withcarboplatin on controlling malignant pleural effusion(MPE).Method A total of 86 cancer patients with appearedPME were selected as our subjected who hospitalized in the First People's Hospital of Shangqiu from Feb.2012to Mar.2013.They were randomly divided into the observation group(43 cases) and the control group(43cases).Patients in the oontrol group were treated with carboplatin at dose of 100 mg/per times which was putinto the thoracic cavity,and patients in the observation group was treated with Aidi injection solution at dose of100 mg/per times beside carbeplatin.The treatment period was 4 weeks.The effect information,quality of lifeand adverse reactions were recorded.Results The total effective rate and survival quality improvement rate inobservation group were 86.05％ (37/43) and 51.16％ (22/43),higher than those in control group (67.44％(19/43),30.23％ (13/43)),and the differences were significant(x2 =4.170,3.903 ; P ＜ 0.05).There wasno significant difference in terms of the related adverse reactions between two groups(P ＞0.05).ConclusionThe therapy of Aidi injection solution plus carboplatin on controlling MPE is proved to be effective and it canimprove patients' quality of life with better safety.Therefore it is worthy of recommendation.

As one of the most catastrophic complications, prosthetic joint infection remains one of the most stubborn medical problems.However, the biomarkers and laboratory tests commonly used often exhibit either a low sensitivity or a low specificity, which makes it still difficult to diagnose.Therefore, we summarize a number of recent new developments home and abroad, and overview from the aspects of serological markers, synovial fluid markers, radionuclide imaging methods, preoperative periprosthetic tissue biopsy, in-traoperative periprosthetic tissue culture, histological analysis of periprosthetic tissue and new molecular technology.

Functional near-infrared spectroscopy is a non-invasive optical brain function detection technology,which can do multi-point measurement of changes on cerebral cortical area corresponding to the oxygenation of the blood hemoglobin and deoxygenated hemoglobin concentration,and then reflect the state of brain cortex function.Its greatest advantage is to allow the subjects head moving in a certain range,especially suitable for children.At present this technology could be applied to clinical pediatric,such as epilepsy,autism,attention deficit/hyperactivity disorder,neonatal disease,neurosurgery and cardiovascular surgery,etc.Functional near-infrared spectroscopy technology will have more applications in the field of research on brain function.

A case of mandible plasma cell myeloma is reported.Plasma cell myeloma is a monoclonal neoplastic proliferation of plasma cells of bone-marrow derivation,usually multi-centric but jaw bones are rarely involved.The treatment requires precise pathological diagno-sis.The prognosis of solitary jaw bone lesions can be good after treatment.