Objective To construct a cDNA expression library of Humulus pollen and provide the basis for screening the major allergenic components and producing recombinant allergen of Humulus pollen.Methods Verdure Humulus pollen were collected and preserved in liquid nitrogen after being sift out.Total RNA was extracted from the Humulus pollen with trizol reagent,and cDNA was synthesized by RT-PCR with purified total RNA.Then the cDNA was digested by SfiⅠ and the fragments smaller than 400bp were removed by chroma spin-400 column,and the fragments longer than 400bp were ligated with ?TripIEx2 Vector.The library was packaged in vitro and a small portion of packaged phage was used to infect E.coli XL1-Blue MRF′ for titration.The diversity of the library and the length of the inserted fragments were analyzed by PCR.Results The cDNA expression library contained 5?10~(5) recombinants and the percentage of recombination was 90%.The average length of inserted cDNA fragments was about 1.02kb.Conclusion The constructed cDNA expression library contains appropriate contents and sizes of cDNA fragments and is qualified for screening target cDNA clone.

Objective:To study the clinical significance of central electric-fetal monitoring system for ante-partum observation and measurement.Methods:The experimental group (253 pregnant women who delivered in our hospital from January,2001 to August,2003) was monitored with the central electric fetal monitoring system.The control group (250 pregnant women who delivered the children in our hospital from July,1998 to September,2000) was not monitered with this system.Then,the results of fetal monitoring and the outcome of prenatal fetuses of the two groups were compared and analyzed.Results:The rate of abnormal FHR was markedly higher in experiment group(30.8%) than in the control group(17.2%),and the rate of variable deceleration in abnormal graph was markedly higher in the experimental group (28.8%) than in the control group (12.4%).There was a significant difference is significant between the two groups.The incidence of asphyxia among newborns was lower in the experimental group (4%) than in the control group (8.4%),and the difference is significant between the two groups.However,there was no significant difference in the rate of cesarean delivery and Simpson forceps delivery between the two groups.Conclusion:Ante-partum monitoring assisted by central electric-fetal monitoring system can improve the prenatal prognosis and increase the rate of cesarean delivery.

Objective:To investigate the sexual concept and sex status of pregnant women so as to instruct pregnant health care and improve living quality.Methods:112 random sample candidates who have mature fetus for delivery in hospital were chosen to investigate and analyze by means of self-edited questionnaires and Eysenck protracted sexual latitude scale test.Results:Both sex frequency and sex continuing time of pregnant women reduced remarkably;their knowledge on sex related to pregnancy was generally poor;average value of Eysenck protracted sexual latitude scale test was 5.25,lying below the average points;professionals and individual professionals have statistics differences in occupation team;the single-mode of sexual intercourse in the sex of pregnant women has the highest percentage.Conclusion:Pregnant women lack the related health care knowledge on sex,and their concept of sex is comparatively conservative,so they can not enjoy full sex and need further improvement.

Objective:Under the background that the army hospitals need to employ civilian nurses in a large quantity,we try to grope a scientific,objective method to choose the nurse who matches post.Methods:Referring to related data and the ritual of talented person evaluation,we chose the evaluation methods including paper test,computer test,personal interview and data integration;arranged the steps of planning,assigning the weight,performing evaluation,integrating the test results,and tracking for feedback;in order to form the mode to test the employed nurses' occupational diathesis.Results:We fulfilled a series of scales for mood and hobby,weight score,quality structure,evaluation method comparison and nurse's professional quality evaluation,etc,and determined the evaluation methods and procedures.Conclusion:Evaluating more than 100 nurses who are newly employed using this new mode,the result demonstrates that this evaluation mode can be easily operated,and is a guide in employing quantities of professional nurses for a hospital.

Animals ; Antineoplastic Agents ; therapeutic use ; Breast Neoplasms ; drug therapy ; genetics ; metabolism ; Cyclin-Dependent Kinase Inhibitor p27 ; Fenretinide ; therapeutic use ; Hepatocyte Nuclear Factor 3-alpha ; genetics ; metabolism ; Humans ; Intracellular Signaling Peptides and Proteins ; metabolism ; Prognosis ; Receptors, Estrogen ; metabolism

Objective To examine the status of and factors influencing medical students' mental health.Methods The China undergraduates' mental health scale and an information questionnaire developed by the authors were administered on 1 205 students selected from Grade 1-3 in chnical medicine,anesthesia,and medical diagnostic programs using cluster sampling.Results The level of medical students' somatization,a~nxiety,depression,social withdrawal,irritabihty,and obsession was higher as compared to the norms for general undergraduates in China.Female medical students showed higher scores than males on 4 aspects:somatization,anxiety,depression,and obsession.Additionally,those belonging to minority nationalities showed higher scores on social withdrawal,irritability,and impulse as compared to those with the Han nationality.Further,students with low professional identity had higher scores on somatization,anxiety,depression,inferiority,irritability,and social withdrawal as compared to those with high professional identity.Conclusion Medical students' mental health is not optimal;therefore,it needs to be addressed by educational departments and educational works.Thus,mental health education,professional counseling,and appropriate interventions need to be conducted for medical students.

Venous thromboembolism is a serious complication of the patients undergoing orthopaedic surgery.Effective diagno-sis and assessment are important methods to predict venous thromboembolismand decrease the incidence of venous thromboenbolism . This paper summaries the advantages and disadvantages of subjective and objective assessment tools to help doctors and nurses find the patients with high risk in the early stage,take individual prevention,and decrease the incidence of venous thromboembolism.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

Objective: To examine the effect of group mental intervention on implicit and explicit aggression of violent juvenile criminals. Methods: A survey investigation and IAT test on implicit aggression were employed to evaluate the aggression of violent juvenile criminals. And the effect of ten times of group intervention on aggression of juvenile criminals was analyzed. Results: After group mental intervention, the implicit aggression was not reduced significantly, but the intervention effect on explicit aggression was significant. Physical aggression and verbal aggression of explicit aggression reduced significantly, but there was no significant difference in rage and hostility. Conclusion: Group mental intervention is an effective method for reducing explicit aggression of violent juvenile criminals.

Objective To analyze the effect of on comprehensive therapy advanced non-small cell lung cancer,and summarize the clinical experience.Methods Based on the comprehensive therapy characteristic,we designed a clinic cases analysis database using the SQL sever database technique.Results In the 102 patients with advanced non-small cell lung cancer in the Xiyuan Hospital,the mean survival period was 11.5 months and the 1 year survival rate was 37.5%.The weight was stable and KPS was increased.There was significant difference in the improvement of mental weariness and hypodynamia,deficient breath and indolence,cough,abundant sputum,pain,anorexia,sputum mixed with blood,constipation,abdominal diarrhea and distention(P