Objective:Under the background that the army hospitals need to employ civilian nurses in a large quantity,we try to grope a scientific,objective method to choose the nurse who matches post.Methods:Referring to related data and the ritual of talented person evaluation,we chose the evaluation methods including paper test,computer test,personal interview and data integration;arranged the steps of planning,assigning the weight,performing evaluation,integrating the test results,and tracking for feedback;in order to form the mode to test the employed nurses' occupational diathesis.Results:We fulfilled a series of scales for mood and hobby,weight score,quality structure,evaluation method comparison and nurse's professional quality evaluation,etc,and determined the evaluation methods and procedures.Conclusion:Evaluating more than 100 nurses who are newly employed using this new mode,the result demonstrates that this evaluation mode can be easily operated,and is a guide in employing quantities of professional nurses for a hospital.

Objective:To study the clinical significance of central electric-fetal monitoring system for ante-partum observation and measurement.Methods:The experimental group (253 pregnant women who delivered in our hospital from January,2001 to August,2003) was monitored with the central electric fetal monitoring system.The control group (250 pregnant women who delivered the children in our hospital from July,1998 to September,2000) was not monitered with this system.Then,the results of fetal monitoring and the outcome of prenatal fetuses of the two groups were compared and analyzed.Results:The rate of abnormal FHR was markedly higher in experiment group(30.8%) than in the control group(17.2%),and the rate of variable deceleration in abnormal graph was markedly higher in the experimental group (28.8%) than in the control group (12.4%).There was a significant difference is significant between the two groups.The incidence of asphyxia among newborns was lower in the experimental group (4%) than in the control group (8.4%),and the difference is significant between the two groups.However,there was no significant difference in the rate of cesarean delivery and Simpson forceps delivery between the two groups.Conclusion:Ante-partum monitoring assisted by central electric-fetal monitoring system can improve the prenatal prognosis and increase the rate of cesarean delivery.

Objective:To investigate the sexual concept and sex status of pregnant women so as to instruct pregnant health care and improve living quality.Methods:112 random sample candidates who have mature fetus for delivery in hospital were chosen to investigate and analyze by means of self-edited questionnaires and Eysenck protracted sexual latitude scale test.Results:Both sex frequency and sex continuing time of pregnant women reduced remarkably;their knowledge on sex related to pregnancy was generally poor;average value of Eysenck protracted sexual latitude scale test was 5.25,lying below the average points;professionals and individual professionals have statistics differences in occupation team;the single-mode of sexual intercourse in the sex of pregnant women has the highest percentage.Conclusion:Pregnant women lack the related health care knowledge on sex,and their concept of sex is comparatively conservative,so they can not enjoy full sex and need further improvement.

Objective To construct a cDNA expression library of Humulus pollen and provide the basis for screening the major allergenic components and producing recombinant allergen of Humulus pollen.Methods Verdure Humulus pollen were collected and preserved in liquid nitrogen after being sift out.Total RNA was extracted from the Humulus pollen with trizol reagent,and cDNA was synthesized by RT-PCR with purified total RNA.Then the cDNA was digested by SfiⅠ and the fragments smaller than 400bp were removed by chroma spin-400 column,and the fragments longer than 400bp were ligated with ?TripIEx2 Vector.The library was packaged in vitro and a small portion of packaged phage was used to infect E.coli XL1-Blue MRF′ for titration.The diversity of the library and the length of the inserted fragments were analyzed by PCR.Results The cDNA expression library contained 5?10~(5) recombinants and the percentage of recombination was 90%.The average length of inserted cDNA fragments was about 1.02kb.Conclusion The constructed cDNA expression library contains appropriate contents and sizes of cDNA fragments and is qualified for screening target cDNA clone.

Objective Early identification of deep vein thrombosis(DVT) risk in patients with joint arthroplasty helps to reduce the incidence of DVT.The study aimed to investigate the effectiveness of Autar risk assessment model in assessing DVT risk in patients with joint arthroplasty.Methods 72 patients with DVT from 10 hospitals from November 2014 to February 2016 were selected as the case group, and 144 non-DVP patients who hospitalized during the same period served as the control group.The medical history, laboratory tests and other related clinical data of the patients were collected, and Autar risk assessment scale was used to evaluate the patients.Multiple logistic regression was applied to analyze the relationship between the risk scale of DVT and the risk of DVT formation.Results 216 patients were included in the research.The Autar score of DVT patients (17.09±2.83) in case group was higher than that of control group (14.25±2.46) and the difference is of statistical significance(P<0.05).The area under the ROC curve of Autar scale in case group was 0.789 (95％CI: 0.719, 0.858,P<0.05).When the ROC curve cutoff point was 15, the sensitivity and the specificity of Autar scale were 88.57％ and 57.90％ respectively.In Autar scale, the main risk factors of DVT incidence were age≥70 years old, BMI≥30, strict bed rest, varicose veins, lower limb injuries and orthopedic surgery.The result of logistic regression analysis showed the difference of Autar risk rating between these two groups was of statistical significance(P<0.05).In comparison with low risk group, the DVT risk of patients with joint arthroplasty at medium risk was of no significant difference (OR=1.178, 95％ CI: 0.733-1.490, P=0.053) while that of patients with joint arthroplasty at high risk was of significant difference (OR=5.536, 95％ CI:3.577-7.132, P<0.001).Conclusion Autar DVT risk assessment scale assessed patients with joint arthroplasty at high risk are high risk group of DVT.The risk of DVT increases with the risk rating in patients with joint arthroplasty.Therefore, Autar risk assessment scale can be applied in the assessment of DVT risk in patients with joint arthroplasty.

Objective:To investigate the acid resistance of tooth hard tissues irradiated by different power outputs of Er,Cr∶YSGG laser.Methods:Samples in laser groups were irradiated using Er,Cr∶ YSGG laser with the power outputs of 2.5,3.5 and 5 W for enamel and 2 W,3 W and 4 W for dentin,respectively.The calcium and phosphate ion dissolved was measured after decalcification in lactate buffer solution for 24 h.The atomic percentage of calcium and phosphate on the surface of samples was examined by X-ray energy dispersive spectroscopy and the morphological changes were investigated by SEM.Results:In all laser groups of enamel samples except 2.5 W group calcium and phosphate ion dissolved less than in control group and block group (P＜0.05).There's no statistic difference between different power groups.Compared with control group and block group,Ca/P ratio increased(P＜0.05).There's no statistic difference of the atomic percentage of Ca and P on the surface of dentin samples between each 2 groups.SEM observation showed that the surface of the laser irradiated samples was rouph,the space among enamel fibers was increased,the dentin around dentinal tuble orifice was protruded and looked like collar flange.Conclusion:Er,Cr∶YSGG laser irradiation with a range of power is effective in increasing acquired acid resistance of dental hard tissue.There was no relationship between laser power outputs and acid resistance.

At present scopolamine is the most powerful single anti-motion sickness drug, but with prominent unwanted side effects. Many attempts had been made to decrease the unwanted side effects, but no any approach was considered to be successful. Based on our working hypothesis that central cholinolytic activity of anticholinergics may not be parallel completely to their side effects, a series of alicyclic amino alcohol esters were designed, synthesized and evaluated. One of the best compounds, phencynonate HCl, was obtained by transesteration of methyl α-phenyl-α-cyclopentyl-α-hydroxy acetate with N-methyl-3-azabicyclo(3,3,1)nonan-9α-ol. In animal models it was demonstrated that at equivalent anti-motion sickness dose the side effects of phencynonate were milder than those of two other central anticholinergic anti-motion sickness drugs scopolamine HBr and difenidol HCl. In clinical trials the overall effectiveness rates for prevention of seasickness and carsickness of phencynonate (oral 2-4 mg/person) was very significantly higher than that of placebo, and also significantly higher than that of difenidol (oral 25-50 mg/person). In self controlled rotatory chair experiments in hospital laboratory, the preventive effects of phencynonate and difenidol in reducing the changes in electronystagmus and electrogastrogram were statistically significant. In another self controlled rotation experiment, phencynonate (2-4 mg/person) and scopolamine (0.3-0.6 mg/person) showed significant anti-motion sickness effects in reducing the gastric electric cycles of electrogastrogram and the Graybiel scores of acute motion sickness and significant inhibitory effects on visual-vestibular interaction dose-dependently. The anti-motion sickness effects of phencynonate 2 and 4 mg were correspondent with those of scopolamine 0.3 and 0.6 mg, respectively. Student pilots with high susceptibility to airsickness were stimulated by Coriolis acceleration. The course of desensitization and habituation to airsickness training in phencynonate group (3 mg/person) was significantly shorter than that of placebo. There was no rebounding in sensitivity to Coriolis stimulation after discontinuing phencynonate, which was reported in case of scopolamine. The side effects of phencynonate HCl were mild dry mouth (9.7%) and drowsiness (9.97%). The incidence of drowsiness is significantly lower than that of difenidol. The side effect of drowsiness was only appeared in aboard ship and bus experiments, but not in PhaseⅠ trial in hospital or in laboratory rotation tests. The incidence of drowsiness of phencynonate was also lower than that of dramamine in aboard tank experiment. Phencynonate could effectively control the acute attack of vertigo, especially Meniere′s disease and positional vertigo. In animal models of Parkinson′s disease and parkinsonism, phencynonate showed morepotent antagonistic effects than clinical common used trihexyphendyl. In summary, phencynonate is a new central anticholinergic anti-motion sickness drug with higher efficacy and lower central inhibitory side effect than difenidol and scopolamine in prevention of motion sickness. Phencynonate HCl was approved on Dec 25,1993 by State Food and Drug Administration of China as a Class Ⅰ new drug for the prevention and treatment of motion sickness in the market in China.

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

DNA repair gene polymorphism can change the functions and efficiency of DNA repair,influence cancer susceptibility.Many studies have been reported that DNA damage repair gene polymorphisms may be related to cancer susceptibility mutation in a variety of tumors and plays an important role in the pathological process.In addition,DNA damage repair genes may interact with other genes,the combined effect of tumor occurrence,development.Lung cancer is the well-studied tumor in this respect.In this paper,DNA damage repair gene XRCC and hOGG1 polymorphisms biological characteristics,these gene single nucleotide polymorphisms as well as cancer susceptibility were reviewed to provide a theoretical basis for the tumor prevention,diagnosis and treatment.

Objective To analyze the clinical characteristics and determine the early diagnosis and treatment of mesenterie venous thrombosis (MVT). Methods Clinieal data of 31 cases with MVT were analyzed retrospectively. Results Of all 31cases,19 patients presented acute MVT. Abdominal pain was the first onset symptom and then progressively exacerbated; peritoneal irritation and ascites developed in 57.9% and 68.4 % patients with sign of ascites respectively. 13 patients were diagnosed by abdominal CT scan or selective mesenterie angiography; the detectable rate of CT for acute MVT was 83.3%. The level of plasma D-Dimer was increased in 93.3% patients with acute MVT. 13 patients underwent surgical treatment, among them,6 cases received anticoagnlafion treatment,4 cases recrudersced and 3 died. 12 chronic MVT patients had no symptoms and were identified through abdominal CT 8 cases developed upper gastrointestinal hemorrhage and 2 died. Conclusion The nonspecifie nature of the abdominal symptoms often delays the diagnosis of MVT and leads to high mortality. Abdominal CT is valuable diagnosis method for MVT and anticongulation treatment and operation are effective managements.